Links between the oncoprotein YB-1 and small non-coding RNAs in breast cancer.

<h4>Background</h4>The nucleic acid-binding protein YB-1, a member of the cold-shock domain protein family, has been implicated in the progression of breast cancer and is associated with poor patient survival. YB-1 has sequence similarity to LIN28, another cold-shock protein family membe...

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Autores principales:	Cherie Blenkiron, Daniel G Hurley, Sandra Fitzgerald, Cristin G Print, Annette Lasham
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dedcf6812fea48ee9e8f342ffb35d28b
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spelling	oai:doaj.org-article:dedcf6812fea48ee9e8f342ffb35d28b2021-11-18T08:45:59ZLinks between the oncoprotein YB-1 and small non-coding RNAs in breast cancer.1932-620310.1371/journal.pone.0080171https://doaj.org/article/dedcf6812fea48ee9e8f342ffb35d28b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24260353/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The nucleic acid-binding protein YB-1, a member of the cold-shock domain protein family, has been implicated in the progression of breast cancer and is associated with poor patient survival. YB-1 has sequence similarity to LIN28, another cold-shock protein family member, which has a role in the regulation of small noncoding RNAs (sncRNAs) including microRNAs (miRNAs). Therefore, to investigate whether there is an association between YB-1 and sncRNAs in breast cancer, we investigated whether sncRNAs were bound by YB-1 in two breast cancer cell lines (luminal A-like and basal cell-like), and whether the abundance of sncRNAs and mRNAs changed in response to experimental reduction of YB-1 expression.<h4>Results</h4>RNA-immunoprecipitation with an anti-YB-1 antibody showed that several sncRNAs are bound by YB-1. Some of these were bound by YB-1 in both breast cancer cell lines; others were cell-line specific. The small RNAs bound by YB-1 were derived from various sncRNA families including miRNAs such as let-7 and miR-320, transfer RNAs, ribosomal RNAs and small nucleolar RNAs (snoRNA). Reducing YB-1 expression altered the abundance of a number of transcripts encoding miRNA biogenesis and processing proteins but did not alter the abundance of mature or precursor miRNAs.<h4>Conclusions</h4>YB-1 binds to specific miRNAs, snoRNAs and tRNA-derived fragments and appears to regulate the expression of miRNA biogenesis and processing machinery. We propose that some of the oncogenic effects of YB-1 in breast cancer may be mediated through its interactions with sncRNAs.Cherie BlenkironDaniel G HurleySandra FitzgeraldCristin G PrintAnnette LashamPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e80171 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cherie Blenkiron Daniel G Hurley Sandra Fitzgerald Cristin G Print Annette Lasham Links between the oncoprotein YB-1 and small non-coding RNAs in breast cancer.
description	<h4>Background</h4>The nucleic acid-binding protein YB-1, a member of the cold-shock domain protein family, has been implicated in the progression of breast cancer and is associated with poor patient survival. YB-1 has sequence similarity to LIN28, another cold-shock protein family member, which has a role in the regulation of small noncoding RNAs (sncRNAs) including microRNAs (miRNAs). Therefore, to investigate whether there is an association between YB-1 and sncRNAs in breast cancer, we investigated whether sncRNAs were bound by YB-1 in two breast cancer cell lines (luminal A-like and basal cell-like), and whether the abundance of sncRNAs and mRNAs changed in response to experimental reduction of YB-1 expression.<h4>Results</h4>RNA-immunoprecipitation with an anti-YB-1 antibody showed that several sncRNAs are bound by YB-1. Some of these were bound by YB-1 in both breast cancer cell lines; others were cell-line specific. The small RNAs bound by YB-1 were derived from various sncRNA families including miRNAs such as let-7 and miR-320, transfer RNAs, ribosomal RNAs and small nucleolar RNAs (snoRNA). Reducing YB-1 expression altered the abundance of a number of transcripts encoding miRNA biogenesis and processing proteins but did not alter the abundance of mature or precursor miRNAs.<h4>Conclusions</h4>YB-1 binds to specific miRNAs, snoRNAs and tRNA-derived fragments and appears to regulate the expression of miRNA biogenesis and processing machinery. We propose that some of the oncogenic effects of YB-1 in breast cancer may be mediated through its interactions with sncRNAs.
format	article
author	Cherie Blenkiron Daniel G Hurley Sandra Fitzgerald Cristin G Print Annette Lasham
author_facet	Cherie Blenkiron Daniel G Hurley Sandra Fitzgerald Cristin G Print Annette Lasham
author_sort	Cherie Blenkiron
title	Links between the oncoprotein YB-1 and small non-coding RNAs in breast cancer.
title_short	Links between the oncoprotein YB-1 and small non-coding RNAs in breast cancer.
title_full	Links between the oncoprotein YB-1 and small non-coding RNAs in breast cancer.
title_fullStr	Links between the oncoprotein YB-1 and small non-coding RNAs in breast cancer.
title_full_unstemmed	Links between the oncoprotein YB-1 and small non-coding RNAs in breast cancer.
title_sort	links between the oncoprotein yb-1 and small non-coding rnas in breast cancer.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/dedcf6812fea48ee9e8f342ffb35d28b
work_keys_str_mv	AT cherieblenkiron linksbetweentheoncoproteinyb1andsmallnoncodingrnasinbreastcancer AT danielghurley linksbetweentheoncoproteinyb1andsmallnoncodingrnasinbreastcancer AT sandrafitzgerald linksbetweentheoncoproteinyb1andsmallnoncodingrnasinbreastcancer AT cristingprint linksbetweentheoncoproteinyb1andsmallnoncodingrnasinbreastcancer AT annettelasham linksbetweentheoncoproteinyb1andsmallnoncodingrnasinbreastcancer
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Links between the oncoprotein YB-1 and small non-coding RNAs in breast cancer.

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