Optoacoustic micro-tomography at 100 volumes per second

Abstract Optical microscopy remains a fundamental tool for modern biological discovery owing to its excellent spatial resolution and versatile contrast in visualizing cellular and sub-cellular structures. Yet, the time domain is paramount for the observation of biological dynamics in living systems....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	X. Luís Deán-Ben, Hernán López-Schier, Daniel Razansky
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dee1eb1c14894143999cae2b2afb852b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:dee1eb1c14894143999cae2b2afb852b
record_format	dspace
spelling	oai:doaj.org-article:dee1eb1c14894143999cae2b2afb852b2021-12-02T16:06:18ZOptoacoustic micro-tomography at 100 volumes per second10.1038/s41598-017-06554-92045-2322https://doaj.org/article/dee1eb1c14894143999cae2b2afb852b2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06554-9https://doaj.org/toc/2045-2322Abstract Optical microscopy remains a fundamental tool for modern biological discovery owing to its excellent spatial resolution and versatile contrast in visualizing cellular and sub-cellular structures. Yet, the time domain is paramount for the observation of biological dynamics in living systems. Commonly, acquisition of microscopy data involves scanning of a spherically- or cylindrically-focused light beam across the imaged volume, which significantly limits temporal resolution in 3D. Additional complications arise from intense light scattering of biological tissues, further restraining the effective penetration depth and field of view of optical microscopy techniques. To overcome these limitations, we devised a fast optoacoustic micro-tomography (OMT) approach based on simultaneous acquisition of 3D image data with a high-density hemispherical ultrasound array having effective detection bandwidth beyond 25 MHz. We demonstrate fast three-dimensional imaging of freely-swimming zebrafish larvae, achieving 3D imaging speed of 100 volumes per second with isotropic spatial resolution approaching the dimensions of large cells across a field of view exceeding 50mm3. As opposed to other microscopy techniques based on optical contrast, OMT resolves optical absorption acoustically using unfocused light excitation. Thus, no penetration barriers are imposed by light scattering in deep tissues, suggesting it as a powerful approach for multi-scale functional and molecular imaging applications.X. Luís Deán-BenHernán López-SchierDaniel RazanskyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q X. Luís Deán-Ben Hernán López-Schier Daniel Razansky Optoacoustic micro-tomography at 100 volumes per second
description	Abstract Optical microscopy remains a fundamental tool for modern biological discovery owing to its excellent spatial resolution and versatile contrast in visualizing cellular and sub-cellular structures. Yet, the time domain is paramount for the observation of biological dynamics in living systems. Commonly, acquisition of microscopy data involves scanning of a spherically- or cylindrically-focused light beam across the imaged volume, which significantly limits temporal resolution in 3D. Additional complications arise from intense light scattering of biological tissues, further restraining the effective penetration depth and field of view of optical microscopy techniques. To overcome these limitations, we devised a fast optoacoustic micro-tomography (OMT) approach based on simultaneous acquisition of 3D image data with a high-density hemispherical ultrasound array having effective detection bandwidth beyond 25 MHz. We demonstrate fast three-dimensional imaging of freely-swimming zebrafish larvae, achieving 3D imaging speed of 100 volumes per second with isotropic spatial resolution approaching the dimensions of large cells across a field of view exceeding 50mm3. As opposed to other microscopy techniques based on optical contrast, OMT resolves optical absorption acoustically using unfocused light excitation. Thus, no penetration barriers are imposed by light scattering in deep tissues, suggesting it as a powerful approach for multi-scale functional and molecular imaging applications.
format	article
author	X. Luís Deán-Ben Hernán López-Schier Daniel Razansky
author_facet	X. Luís Deán-Ben Hernán López-Schier Daniel Razansky
author_sort	X. Luís Deán-Ben
title	Optoacoustic micro-tomography at 100 volumes per second
title_short	Optoacoustic micro-tomography at 100 volumes per second
title_full	Optoacoustic micro-tomography at 100 volumes per second
title_fullStr	Optoacoustic micro-tomography at 100 volumes per second
title_full_unstemmed	Optoacoustic micro-tomography at 100 volumes per second
title_sort	optoacoustic micro-tomography at 100 volumes per second
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/dee1eb1c14894143999cae2b2afb852b
work_keys_str_mv	AT xluisdeanben optoacousticmicrotomographyat100volumespersecond AT hernanlopezschier optoacousticmicrotomographyat100volumespersecond AT danielrazansky optoacousticmicrotomographyat100volumespersecond
_version_	1718385061265408000

Optoacoustic micro-tomography at 100 volumes per second

Ejemplares similares