Investigation of the radiopacity and cytotoxicity of Albo-Dent - novel strontium carbonate incorporated calcium silicate based dental cement

Introduction: Calcium silicate (CS) dental cements have numerous clinical indications in dentistry including pulp capping, root end surgery, perforation repair and apexification/apexogenesis treatment. Materials and methods: Novel CS based dental cement with incorporation of SrCO3 radiopacifier name...

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Autores principales:	Despotović Ana, Antonijević Đorđe, Ilić Dragan, Zogović Nevena, Jokanović Vukoman
Formato:	article
Lenguaje:	EN SR
Publicado:	Serbian Medical Society - Dental Section, Belgrade 2021
Materias:	calcium silicate strontium carbonate radiopacity dental materials biocompatibility endodontics Dentistry RK1-715
Acceso en línea:	https://doaj.org/article/dee492ae144748f7b0332c783d2d31f8
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spelling	oai:doaj.org-article:dee492ae144748f7b0332c783d2d31f82021-12-05T21:33:39ZInvestigation of the radiopacity and cytotoxicity of Albo-Dent - novel strontium carbonate incorporated calcium silicate based dental cement0039-17431452-370110.2298/SGS2102068Dhttps://doaj.org/article/dee492ae144748f7b0332c783d2d31f82021-01-01T00:00:00Zhttps://scindeks-clanci.ceon.rs/data/pdf/0039-1743/2021/0039-17432102068D.pdfhttps://doaj.org/toc/0039-1743https://doaj.org/toc/1452-3701Introduction: Calcium silicate (CS) dental cements have numerous clinical indications in dentistry including pulp capping, root end surgery, perforation repair and apexification/apexogenesis treatment. Materials and methods: Novel CS based dental cement with incorporation of SrCO3 radiopacifier named ALBO-DENT was used as an experimental cement material while Portland cement (Aalborg, Denmark) and ProRoot MTA (Tulsa Dental, USA) were used as controls. The radiopacity evaluation was performed using digital Trophy Radiographic system with an intention to precisely determine the minimum of radiopaque agent needed to confer to ISO radiopacity requirement. Thereafter, biocompatibility of material was tested in in vitro conditions in mouse fibrosarcoma L929 cell culture treated with materials' extracts. Cell morphology was observed using phase-contrast microscopy, while cell viability was measured using crystal violet (CV) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assays. Results: Radiopacity evaluation revealed that 30%wt addition of SrCO3 was necessary to achieve satisfactory radiopacity (3.45 mm Al). Cytotoxicity analysis using CV and MTT assays revealed that pure extracts of ALBO-DENT presented superior biocompatibility when compared to PC and MTA controls while serial dilutions of experimental cements' extracts as well as that of PC and MTA did not influence L929 cell viability. Conclusions Novel formulation of CS cement - ALBO-DENT presented satisfactory radiopacity and adequate biocompatibility.Despotović AnaAntonijević ĐorđeIlić DraganZogović NevenaJokanović VukomanSerbian Medical Society - Dental Section, Belgradearticlecalcium silicatestrontium carbonateradiopacitydental materials biocompatibilityendodonticsDentistryRK1-715ENSRStomatološki glasnik Srbije, Vol 68, Iss 2, Pp 68-78 (2021)
institution	DOAJ
collection	DOAJ
language	EN SR
topic	calcium silicate strontium carbonate radiopacity dental materials biocompatibility endodontics Dentistry RK1-715
spellingShingle	calcium silicate strontium carbonate radiopacity dental materials biocompatibility endodontics Dentistry RK1-715 Despotović Ana Antonijević Đorđe Ilić Dragan Zogović Nevena Jokanović Vukoman Investigation of the radiopacity and cytotoxicity of Albo-Dent - novel strontium carbonate incorporated calcium silicate based dental cement
description	Introduction: Calcium silicate (CS) dental cements have numerous clinical indications in dentistry including pulp capping, root end surgery, perforation repair and apexification/apexogenesis treatment. Materials and methods: Novel CS based dental cement with incorporation of SrCO3 radiopacifier named ALBO-DENT was used as an experimental cement material while Portland cement (Aalborg, Denmark) and ProRoot MTA (Tulsa Dental, USA) were used as controls. The radiopacity evaluation was performed using digital Trophy Radiographic system with an intention to precisely determine the minimum of radiopaque agent needed to confer to ISO radiopacity requirement. Thereafter, biocompatibility of material was tested in in vitro conditions in mouse fibrosarcoma L929 cell culture treated with materials' extracts. Cell morphology was observed using phase-contrast microscopy, while cell viability was measured using crystal violet (CV) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assays. Results: Radiopacity evaluation revealed that 30%wt addition of SrCO3 was necessary to achieve satisfactory radiopacity (3.45 mm Al). Cytotoxicity analysis using CV and MTT assays revealed that pure extracts of ALBO-DENT presented superior biocompatibility when compared to PC and MTA controls while serial dilutions of experimental cements' extracts as well as that of PC and MTA did not influence L929 cell viability. Conclusions Novel formulation of CS cement - ALBO-DENT presented satisfactory radiopacity and adequate biocompatibility.
format	article
author	Despotović Ana Antonijević Đorđe Ilić Dragan Zogović Nevena Jokanović Vukoman
author_facet	Despotović Ana Antonijević Đorđe Ilić Dragan Zogović Nevena Jokanović Vukoman
author_sort	Despotović Ana
title	Investigation of the radiopacity and cytotoxicity of Albo-Dent - novel strontium carbonate incorporated calcium silicate based dental cement
title_short	Investigation of the radiopacity and cytotoxicity of Albo-Dent - novel strontium carbonate incorporated calcium silicate based dental cement
title_full	Investigation of the radiopacity and cytotoxicity of Albo-Dent - novel strontium carbonate incorporated calcium silicate based dental cement
title_fullStr	Investigation of the radiopacity and cytotoxicity of Albo-Dent - novel strontium carbonate incorporated calcium silicate based dental cement
title_full_unstemmed	Investigation of the radiopacity and cytotoxicity of Albo-Dent - novel strontium carbonate incorporated calcium silicate based dental cement
title_sort	investigation of the radiopacity and cytotoxicity of albo-dent - novel strontium carbonate incorporated calcium silicate based dental cement
publisher	Serbian Medical Society - Dental Section, Belgrade
publishDate	2021
url	https://doaj.org/article/dee492ae144748f7b0332c783d2d31f8
work_keys_str_mv	AT despotovicana investigationoftheradiopacityandcytotoxicityofalbodentnovelstrontiumcarbonateincorporatedcalciumsilicatebaseddentalcement AT antonijevicđorđe investigationoftheradiopacityandcytotoxicityofalbodentnovelstrontiumcarbonateincorporatedcalciumsilicatebaseddentalcement AT ilicdragan investigationoftheradiopacityandcytotoxicityofalbodentnovelstrontiumcarbonateincorporatedcalciumsilicatebaseddentalcement AT zogovicnevena investigationoftheradiopacityandcytotoxicityofalbodentnovelstrontiumcarbonateincorporatedcalciumsilicatebaseddentalcement AT jokanovicvukoman investigationoftheradiopacityandcytotoxicityofalbodentnovelstrontiumcarbonateincorporatedcalciumsilicatebaseddentalcement
_version_	1718370976279822336

Investigation of the radiopacity and cytotoxicity of Albo-Dent - novel strontium carbonate incorporated calcium silicate based dental cement

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