Robust adaptive optics for localization microscopy deep in complex tissue
It is difficult to apply SMLM to complex biological tissues. Here the authors report REALM, Robust and Effective Adaptive Optics in Localisation Microscopy, to improve SMLM in tissue and use this to resolve the organisation of spectrin in the axon initial segment in brain tissue.
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Nature Portfolio
2021
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oai:doaj.org-article:dee4cf76855b40cbb4c28ab779681f9f2021-12-02T15:02:50ZRobust adaptive optics for localization microscopy deep in complex tissue10.1038/s41467-021-23647-22041-1723https://doaj.org/article/dee4cf76855b40cbb4c28ab779681f9f2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-23647-2https://doaj.org/toc/2041-1723It is difficult to apply SMLM to complex biological tissues. Here the authors report REALM, Robust and Effective Adaptive Optics in Localisation Microscopy, to improve SMLM in tissue and use this to resolve the organisation of spectrin in the axon initial segment in brain tissue.Marijn E. SiemonsNaomi A. K. HanemaaijerMaarten H. P. KoleLukas C. KapiteinNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-9 (2021) |
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Science Q Marijn E. Siemons Naomi A. K. Hanemaaijer Maarten H. P. Kole Lukas C. Kapitein Robust adaptive optics for localization microscopy deep in complex tissue |
description |
It is difficult to apply SMLM to complex biological tissues. Here the authors report REALM, Robust and Effective Adaptive Optics in Localisation Microscopy, to improve SMLM in tissue and use this to resolve the organisation of spectrin in the axon initial segment in brain tissue. |
format |
article |
author |
Marijn E. Siemons Naomi A. K. Hanemaaijer Maarten H. P. Kole Lukas C. Kapitein |
author_facet |
Marijn E. Siemons Naomi A. K. Hanemaaijer Maarten H. P. Kole Lukas C. Kapitein |
author_sort |
Marijn E. Siemons |
title |
Robust adaptive optics for localization microscopy deep in complex tissue |
title_short |
Robust adaptive optics for localization microscopy deep in complex tissue |
title_full |
Robust adaptive optics for localization microscopy deep in complex tissue |
title_fullStr |
Robust adaptive optics for localization microscopy deep in complex tissue |
title_full_unstemmed |
Robust adaptive optics for localization microscopy deep in complex tissue |
title_sort |
robust adaptive optics for localization microscopy deep in complex tissue |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doaj.org/article/dee4cf76855b40cbb4c28ab779681f9f |
work_keys_str_mv |
AT marijnesiemons robustadaptiveopticsforlocalizationmicroscopydeepincomplextissue AT naomiakhanemaaijer robustadaptiveopticsforlocalizationmicroscopydeepincomplextissue AT maartenhpkole robustadaptiveopticsforlocalizationmicroscopydeepincomplextissue AT lukasckapitein robustadaptiveopticsforlocalizationmicroscopydeepincomplextissue |
_version_ |
1718389107762135040 |