Vegetal diamine oxidase alleviates histamine-induced contraction of colonic muscles

Abstract Excess of histamine in gut lumen generates a pronounced gastrointestinal discomfort, which may include diarrhea and peristalsis dysfunctions. Deleterious effects of histamine can be alleviated with antihistamine drugs targeting histamine receptors. However, many antihistamine agents come wi...

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Autores principales:	Armelle Tchoumi Neree, Rodolphe Soret, Lucia Marcocci, Paola Pietrangeli, Nicolas Pilon, Mircea Alexandru Mateescu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/defcbb39185343958456791d41819c58
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spelling	oai:doaj.org-article:defcbb39185343958456791d41819c582021-12-02T15:11:53ZVegetal diamine oxidase alleviates histamine-induced contraction of colonic muscles10.1038/s41598-020-78134-32045-2322https://doaj.org/article/defcbb39185343958456791d41819c582020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78134-3https://doaj.org/toc/2045-2322Abstract Excess of histamine in gut lumen generates a pronounced gastrointestinal discomfort, which may include diarrhea and peristalsis dysfunctions. Deleterious effects of histamine can be alleviated with antihistamine drugs targeting histamine receptors. However, many antihistamine agents come with various undesirable side effects. Vegetal diamine oxidase (vDAO) might be a relevant alternative owing to its histaminase activity. Mammalian intestinal mucosa contains an endogenous DAO, yet possessing lower activity compared to that of vDAO preparation. Moreover, in several pathological conditions such as inflammatory bowel disease and irritable bowel syndrome, this endogenous DAO enzyme can be lost or inactivated. Here, we tested the therapeutic potential of vDAO by focusing on the well-known effect of histamine on gut motility. Using ex vivo and in vitro assays, we found that vDAO is more potent than commercial anti-histamine drugs at inhibiting histamine-induced contraction of murine distal colon muscles. We also identified pyridoxal 5′-phosphate (the biologically active form of vitamin B6) as an effective enhancer of vDAO antispasmodic activity. Furthermore, we discovered that rectally administered vDAO can be retained on gut mucosa and remain active. These observations make administration of vDAO in the gut lumen a valid alternative treatment for histamine-induced intestinal dysfunctions.Armelle Tchoumi NereeRodolphe SoretLucia MarcocciPaola PietrangeliNicolas PilonMircea Alexandru MateescuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Armelle Tchoumi Neree Rodolphe Soret Lucia Marcocci Paola Pietrangeli Nicolas Pilon Mircea Alexandru Mateescu Vegetal diamine oxidase alleviates histamine-induced contraction of colonic muscles
description	Abstract Excess of histamine in gut lumen generates a pronounced gastrointestinal discomfort, which may include diarrhea and peristalsis dysfunctions. Deleterious effects of histamine can be alleviated with antihistamine drugs targeting histamine receptors. However, many antihistamine agents come with various undesirable side effects. Vegetal diamine oxidase (vDAO) might be a relevant alternative owing to its histaminase activity. Mammalian intestinal mucosa contains an endogenous DAO, yet possessing lower activity compared to that of vDAO preparation. Moreover, in several pathological conditions such as inflammatory bowel disease and irritable bowel syndrome, this endogenous DAO enzyme can be lost or inactivated. Here, we tested the therapeutic potential of vDAO by focusing on the well-known effect of histamine on gut motility. Using ex vivo and in vitro assays, we found that vDAO is more potent than commercial anti-histamine drugs at inhibiting histamine-induced contraction of murine distal colon muscles. We also identified pyridoxal 5′-phosphate (the biologically active form of vitamin B6) as an effective enhancer of vDAO antispasmodic activity. Furthermore, we discovered that rectally administered vDAO can be retained on gut mucosa and remain active. These observations make administration of vDAO in the gut lumen a valid alternative treatment for histamine-induced intestinal dysfunctions.
format	article
author	Armelle Tchoumi Neree Rodolphe Soret Lucia Marcocci Paola Pietrangeli Nicolas Pilon Mircea Alexandru Mateescu
author_facet	Armelle Tchoumi Neree Rodolphe Soret Lucia Marcocci Paola Pietrangeli Nicolas Pilon Mircea Alexandru Mateescu
author_sort	Armelle Tchoumi Neree
title	Vegetal diamine oxidase alleviates histamine-induced contraction of colonic muscles
title_short	Vegetal diamine oxidase alleviates histamine-induced contraction of colonic muscles
title_full	Vegetal diamine oxidase alleviates histamine-induced contraction of colonic muscles
title_fullStr	Vegetal diamine oxidase alleviates histamine-induced contraction of colonic muscles
title_full_unstemmed	Vegetal diamine oxidase alleviates histamine-induced contraction of colonic muscles
title_sort	vegetal diamine oxidase alleviates histamine-induced contraction of colonic muscles
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/defcbb39185343958456791d41819c58
work_keys_str_mv	AT armelletchoumineree vegetaldiamineoxidasealleviateshistamineinducedcontractionofcolonicmuscles AT rodolphesoret vegetaldiamineoxidasealleviateshistamineinducedcontractionofcolonicmuscles AT luciamarcocci vegetaldiamineoxidasealleviateshistamineinducedcontractionofcolonicmuscles AT paolapietrangeli vegetaldiamineoxidasealleviateshistamineinducedcontractionofcolonicmuscles AT nicolaspilon vegetaldiamineoxidasealleviateshistamineinducedcontractionofcolonicmuscles AT mirceaalexandrumateescu vegetaldiamineoxidasealleviateshistamineinducedcontractionofcolonicmuscles
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Vegetal diamine oxidase alleviates histamine-induced contraction of colonic muscles

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