Annihilation of Leishmania by daylight responsive ZnO nanoparticles: a temporal relationship of reactive oxygen species-induced lipid and protein oxidation

Akhtar Nadhman,1–3 Malik Ihsanullah Khan,1,2 Samina Nazir,4 Momin Khan,1,5 Gul Shahnaz,6 Abida Raza,2 Dilawar Farhan Shams,7 Masoom Yasinzai1,3 1Department of Biotechnology, Faculty of Biological Sciences, Quaid-i-Azam University, 2Nuclear Medicine Oncology and Radiotherapy Institute, 3Ce...

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Autores principales:	Nadhman A, Khan MI, Nazir S, Khan M, Shahnaz G, Raza A, Shams DF, Yasinzai M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Lipid peroxidation Protein oxidation Leishmania tropica Zinc oxide (ZnO) Nanoparticles Reactive oxygen species (ROS) Photodynamic therapy (PDT) Doping Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/df0048fa296641f48e801b9581b3a201
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spelling	oai:doaj.org-article:df0048fa296641f48e801b9581b3a2012021-12-02T05:02:05ZAnnihilation of Leishmania by daylight responsive ZnO nanoparticles: a temporal relationship of reactive oxygen species-induced lipid and protein oxidation1178-2013https://doaj.org/article/df0048fa296641f48e801b9581b3a2012016-05-01T00:00:00Zhttps://www.dovepress.com/annihilation-of-leishmania-by-daylight-responsive-zno-nanoparticles-a--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Akhtar Nadhman,1–3 Malik Ihsanullah Khan,1,2 Samina Nazir,4 Momin Khan,1,5 Gul Shahnaz,6 Abida Raza,2 Dilawar Farhan Shams,7 Masoom Yasinzai1,3 1Department of Biotechnology, Faculty of Biological Sciences, Quaid-i-Azam University, 2Nuclear Medicine Oncology and Radiotherapy Institute, 3Centre for Interdisciplinary Research in Basic Sciences (CIRBS), International Islamic University, 4Nanosciences and Catalysis Division, National Centre for Physics, Quaid-i-Azam University Campus, Islamabad, 5Department of Microbiology, Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, 6Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 7Department of Environmental Sciences, Abdul Wali Khan University Mardan, Mardan, Pakistan Abstract: Lipid and protein oxidation are well-known manifestations of free radical activity and oxidative stress. The current study investigated extermination of Leishmania tropica promastigotes induced by lipid and protein oxidation with reactive oxygen species produced by PEGylated metal-based nanoparticles. The synthesized photodynamic therapy-based doped and nondoped zinc oxide nanoparticles were activated in daylight that produced reactive oxygen species in the immediate environment. Lipid and protein oxidation did not occur in dark. The major lipid peroxidation derivatives comprised of conjugated dienes, lipid hydroperoxides, and malondialdehyde whereas water, ethane, methanol, and ethanol were found as the end products. Proteins were oxidized to carbonyls, hydroperoxides, and thiol degrading products. Interestingly, lipid hydroperoxides were produced by more than twofold of the protein hydroperoxides, indicating higher degradation of lipids compared to proteins. The in vitro evidence represented a significant contribution of the involvement of both lipid and protein oxidation in the annihilated antipromastigote effect of nanoparticles. Keywords: lipid peroxidation, protein oxidation, Leishmania tropica, zinc oxide (ZnO), nanoparticles, reactive oxygen species (ROS), photodynamic therapy (PDT), dopingNadhman AKhan MINazir SKhan MShahnaz GRaza AShams DFYasinzai MDove Medical PressarticleLipid peroxidationProtein oxidationLeishmania tropicaZinc oxide (ZnO)NanoparticlesReactive oxygen species (ROS)Photodynamic therapy (PDT)DopingMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 2451-2461 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Lipid peroxidation Protein oxidation Leishmania tropica Zinc oxide (ZnO) Nanoparticles Reactive oxygen species (ROS) Photodynamic therapy (PDT) Doping Medicine (General) R5-920
spellingShingle	Lipid peroxidation Protein oxidation Leishmania tropica Zinc oxide (ZnO) Nanoparticles Reactive oxygen species (ROS) Photodynamic therapy (PDT) Doping Medicine (General) R5-920 Nadhman A Khan MI Nazir S Khan M Shahnaz G Raza A Shams DF Yasinzai M Annihilation of Leishmania by daylight responsive ZnO nanoparticles: a temporal relationship of reactive oxygen species-induced lipid and protein oxidation
description	Akhtar Nadhman,1–3 Malik Ihsanullah Khan,1,2 Samina Nazir,4 Momin Khan,1,5 Gul Shahnaz,6 Abida Raza,2 Dilawar Farhan Shams,7 Masoom Yasinzai1,3 1Department of Biotechnology, Faculty of Biological Sciences, Quaid-i-Azam University, 2Nuclear Medicine Oncology and Radiotherapy Institute, 3Centre for Interdisciplinary Research in Basic Sciences (CIRBS), International Islamic University, 4Nanosciences and Catalysis Division, National Centre for Physics, Quaid-i-Azam University Campus, Islamabad, 5Department of Microbiology, Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, 6Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 7Department of Environmental Sciences, Abdul Wali Khan University Mardan, Mardan, Pakistan Abstract: Lipid and protein oxidation are well-known manifestations of free radical activity and oxidative stress. The current study investigated extermination of Leishmania tropica promastigotes induced by lipid and protein oxidation with reactive oxygen species produced by PEGylated metal-based nanoparticles. The synthesized photodynamic therapy-based doped and nondoped zinc oxide nanoparticles were activated in daylight that produced reactive oxygen species in the immediate environment. Lipid and protein oxidation did not occur in dark. The major lipid peroxidation derivatives comprised of conjugated dienes, lipid hydroperoxides, and malondialdehyde whereas water, ethane, methanol, and ethanol were found as the end products. Proteins were oxidized to carbonyls, hydroperoxides, and thiol degrading products. Interestingly, lipid hydroperoxides were produced by more than twofold of the protein hydroperoxides, indicating higher degradation of lipids compared to proteins. The in vitro evidence represented a significant contribution of the involvement of both lipid and protein oxidation in the annihilated antipromastigote effect of nanoparticles. Keywords: lipid peroxidation, protein oxidation, Leishmania tropica, zinc oxide (ZnO), nanoparticles, reactive oxygen species (ROS), photodynamic therapy (PDT), doping
format	article
author	Nadhman A Khan MI Nazir S Khan M Shahnaz G Raza A Shams DF Yasinzai M
author_facet	Nadhman A Khan MI Nazir S Khan M Shahnaz G Raza A Shams DF Yasinzai M
author_sort	Nadhman A
title	Annihilation of Leishmania by daylight responsive ZnO nanoparticles: a temporal relationship of reactive oxygen species-induced lipid and protein oxidation
title_short	Annihilation of Leishmania by daylight responsive ZnO nanoparticles: a temporal relationship of reactive oxygen species-induced lipid and protein oxidation
title_full	Annihilation of Leishmania by daylight responsive ZnO nanoparticles: a temporal relationship of reactive oxygen species-induced lipid and protein oxidation
title_fullStr	Annihilation of Leishmania by daylight responsive ZnO nanoparticles: a temporal relationship of reactive oxygen species-induced lipid and protein oxidation
title_full_unstemmed	Annihilation of Leishmania by daylight responsive ZnO nanoparticles: a temporal relationship of reactive oxygen species-induced lipid and protein oxidation
title_sort	annihilation of leishmania by daylight responsive zno nanoparticles: a temporal relationship of reactive oxygen species-induced lipid and protein oxidation
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/df0048fa296641f48e801b9581b3a201
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Annihilation of Leishmania by daylight responsive ZnO nanoparticles: a temporal relationship of reactive oxygen species-induced lipid and protein oxidation

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