Trypanosoma cruzi: role of δ-amastin on extracellular amastigote cell invasion and differentiation.

Trypanosoma cruzi is a protozoan parasite that comprises different phylogenetic groups and is the causative agent of Chagas' disease. Different T. cruzi strains present differences in infectivity in in vitro and in vivo experimental models, which are likely related to the expression of differen...

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Autores principales:	Mário C Cruz, Normanda Souza-Melo, Claudio Vieira da Silva, Wanderson Duarte Darocha, Diana Bahia, Patrícia R Araújo, Santuza R Teixeira, Renato A Mortara
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/df034e8972b34e3da3b8de823a52f946
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spelling	oai:doaj.org-article:df034e8972b34e3da3b8de823a52f9462021-11-18T08:04:42ZTrypanosoma cruzi: role of δ-amastin on extracellular amastigote cell invasion and differentiation.1932-620310.1371/journal.pone.0051804https://doaj.org/article/df034e8972b34e3da3b8de823a52f9462012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23272170/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Trypanosoma cruzi is a protozoan parasite that comprises different phylogenetic groups and is the causative agent of Chagas' disease. Different T. cruzi strains present differences in infectivity in in vitro and in vivo experimental models, which are likely related to the expression of different virulence factors. Amastin is a surface glycoprotein abundantly expressed on the intracellular mammalian amastigote form of the parasite. In this study, we showed that a highly infective strain (G strain) of extracellular amastigote (EA) invasive forms expressed reduced RNA levels of amastin compared to a less infective strain (CL). The treatment of HeLa cells with recombinant δ-amastin reduced infectivity of EA forms. However, the ectopic expression of δ-amastin accelerated amastigote differentiation into trypomastigotes. Corroborating the virulence behavior in association with amastin expression, the EAs overexpressing amastin were precociously and robustly observed in the liver of susceptible mouse strains (A/JUnib), whereas parasitemia was never detected in in vivo assays. This is the first report on the regulatory role of amastin in the course of both in vitro and in vivo T. cruzi infection.Mário C CruzNormanda Souza-MeloClaudio Vieira da SilvaWanderson Duarte DarochaDiana BahiaPatrícia R AraújoSantuza R TeixeiraRenato A MortaraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e51804 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mário C Cruz Normanda Souza-Melo Claudio Vieira da Silva Wanderson Duarte Darocha Diana Bahia Patrícia R Araújo Santuza R Teixeira Renato A Mortara Trypanosoma cruzi: role of δ-amastin on extracellular amastigote cell invasion and differentiation.
description	Trypanosoma cruzi is a protozoan parasite that comprises different phylogenetic groups and is the causative agent of Chagas' disease. Different T. cruzi strains present differences in infectivity in in vitro and in vivo experimental models, which are likely related to the expression of different virulence factors. Amastin is a surface glycoprotein abundantly expressed on the intracellular mammalian amastigote form of the parasite. In this study, we showed that a highly infective strain (G strain) of extracellular amastigote (EA) invasive forms expressed reduced RNA levels of amastin compared to a less infective strain (CL). The treatment of HeLa cells with recombinant δ-amastin reduced infectivity of EA forms. However, the ectopic expression of δ-amastin accelerated amastigote differentiation into trypomastigotes. Corroborating the virulence behavior in association with amastin expression, the EAs overexpressing amastin were precociously and robustly observed in the liver of susceptible mouse strains (A/JUnib), whereas parasitemia was never detected in in vivo assays. This is the first report on the regulatory role of amastin in the course of both in vitro and in vivo T. cruzi infection.
format	article
author	Mário C Cruz Normanda Souza-Melo Claudio Vieira da Silva Wanderson Duarte Darocha Diana Bahia Patrícia R Araújo Santuza R Teixeira Renato A Mortara
author_facet	Mário C Cruz Normanda Souza-Melo Claudio Vieira da Silva Wanderson Duarte Darocha Diana Bahia Patrícia R Araújo Santuza R Teixeira Renato A Mortara
author_sort	Mário C Cruz
title	Trypanosoma cruzi: role of δ-amastin on extracellular amastigote cell invasion and differentiation.
title_short	Trypanosoma cruzi: role of δ-amastin on extracellular amastigote cell invasion and differentiation.
title_full	Trypanosoma cruzi: role of δ-amastin on extracellular amastigote cell invasion and differentiation.
title_fullStr	Trypanosoma cruzi: role of δ-amastin on extracellular amastigote cell invasion and differentiation.
title_full_unstemmed	Trypanosoma cruzi: role of δ-amastin on extracellular amastigote cell invasion and differentiation.
title_sort	trypanosoma cruzi: role of δ-amastin on extracellular amastigote cell invasion and differentiation.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/df034e8972b34e3da3b8de823a52f946
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Trypanosoma cruzi: role of δ-amastin on extracellular amastigote cell invasion and differentiation.

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