Multiple Plasmid Vectors Mediate the Spread of <italic toggle="yes">fosA3</italic> in Extended-Spectrum-β-Lactamase-Producing <italic toggle="yes">Enterobacterales</italic> Isolates from Retail Vegetables in China

ABSTRACT The plasmid-mediated fosfomycin resistance gene fosA3 has been detected in Enterobacterales from various sources but has rarely been reported in vegetables. In this study, the aim was to investigate the prevalence of and, subsequently, to characterize fosA3-positive Enterobacterales isolate...

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Autores principales:	Luchao Lv, Xiuyu Huang, Jing Wang, Ying Huang, Xun Gao, Yiyun Liu, Qiaoli Zhou, Qianhui Zhang, Jun Yang, Jian-Ying Guo, Jian-Hua Liu
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	fosA3 ESBL-producing Enterobacterales retail vegetable plasmids Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/df03be5b35134ec28e41a076adb1da9d
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spelling	oai:doaj.org-article:df03be5b35134ec28e41a076adb1da9d2021-11-15T15:30:51ZMultiple Plasmid Vectors Mediate the Spread of <italic toggle="yes">fosA3</italic> in Extended-Spectrum-β-Lactamase-Producing <italic toggle="yes">Enterobacterales</italic> Isolates from Retail Vegetables in China10.1128/mSphere.00507-202379-5042https://doaj.org/article/df03be5b35134ec28e41a076adb1da9d2020-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00507-20https://doaj.org/toc/2379-5042ABSTRACT The plasmid-mediated fosfomycin resistance gene fosA3 has been detected in Enterobacterales from various sources but has rarely been reported in vegetables. In this study, the aim was to investigate the prevalence of and, subsequently, to characterize fosA3-positive Enterobacterales isolates from retail vegetables. Seventeen (7.3%) fosA3-carrying strains were identified from 233 extended-spectrum-β-lactamase-producing Enterobacterales isolates from vegetables. All 17 isolates, including six Escherichia coli, seven Klebsiella pneumoniae, two Raoultella ornithinolytica, and two Citrobacter freundii isolates, carried blaCTX-M. S1-nuclease pulsed-field gel electrophoresis (S1-PFGE) and hybridization confirmed that the fosA3 genes in 16 isolates were located on plasmids ranging in size from ∼40 kb to ∼250 kb, except one located on chromosome of C. freundii. All the fosA3-carrying plasmids from 16 fosA3-positive isolates were successfully transferred into the recipient bacteria by transformation or conjugation. In agreement with data determined with isolates from food animals, the IncHI2/ST3 and IncN-F33:A-:B-/F33:A-:B plasmids were the main vectors of fosA3 in E. coli. Additionally, F24:A-:B1, IncFIIK-IncR, IncFIIS, IncR, and two untypeable plasmids were found for the first time to be vectors for fosA3 in Enterobacterales. The genetic contexts of fosA3 in 15 Enterobacterales isolates differed due to insertion and/or loss of molecular modules mediated by mobile elements. However, all fosA3 genes were flanked by IS26, as commonly observed in other fosA3-carrying plasmids. Here, we report a high rate of detection of fosA3 genes, mediated by multiple plasmid vectors, in ESBL-producing Enterobacterales from retail vegetables. FosA3-producing Enterobacterales could be transmitted to the human body by direct contact or consumption of vegetables, which might pose a potential threat to public health. IMPORTANCE This report provides important information on the transmission and epidemiology of fosA3 among Enterobacterales isolates from vegetables. The rate of occurrence of fosA3 in ESBL-producing Enterobacterales from retail vegetables is high, and fosA3 was found to be carried by diverse plasmids. Some novel genetic contexts of fosA3 and novel fosA3-carrying plasmids, including several plasmid types common in K. pneumoniae, were identified, increasing the number of known transfer vectors for the fosA3 gene and reflecting the complexity of fosA3 transmission in Enterobacterales. The capture of fosA3 by the resident plasmid of K. pneumoniae will accelerate the spread of fosA3 in K. pneumoniae, one of the most pathogenic species in clinical medicine. Considering the clinical importance of fosfomycin, and the fact that vegetables are directly consumed, the fosfomycin resistance genes present a risk of transmission to the human body through the food chain and thus pose a threat to public health.Luchao LvXiuyu HuangJing WangYing HuangXun GaoYiyun LiuQiaoli ZhouQianhui ZhangJun YangJian-Ying GuoJian-Hua LiuAmerican Society for MicrobiologyarticlefosA3ESBL-producing Enterobacteralesretail vegetableplasmidsMicrobiologyQR1-502ENmSphere, Vol 5, Iss 4 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	fosA3 ESBL-producing Enterobacterales retail vegetable plasmids Microbiology QR1-502
spellingShingle	fosA3 ESBL-producing Enterobacterales retail vegetable plasmids Microbiology QR1-502 Luchao Lv Xiuyu Huang Jing Wang Ying Huang Xun Gao Yiyun Liu Qiaoli Zhou Qianhui Zhang Jun Yang Jian-Ying Guo Jian-Hua Liu Multiple Plasmid Vectors Mediate the Spread of <italic toggle="yes">fosA3</italic> in Extended-Spectrum-β-Lactamase-Producing <italic toggle="yes">Enterobacterales</italic> Isolates from Retail Vegetables in China
description	ABSTRACT The plasmid-mediated fosfomycin resistance gene fosA3 has been detected in Enterobacterales from various sources but has rarely been reported in vegetables. In this study, the aim was to investigate the prevalence of and, subsequently, to characterize fosA3-positive Enterobacterales isolates from retail vegetables. Seventeen (7.3%) fosA3-carrying strains were identified from 233 extended-spectrum-β-lactamase-producing Enterobacterales isolates from vegetables. All 17 isolates, including six Escherichia coli, seven Klebsiella pneumoniae, two Raoultella ornithinolytica, and two Citrobacter freundii isolates, carried blaCTX-M. S1-nuclease pulsed-field gel electrophoresis (S1-PFGE) and hybridization confirmed that the fosA3 genes in 16 isolates were located on plasmids ranging in size from ∼40 kb to ∼250 kb, except one located on chromosome of C. freundii. All the fosA3-carrying plasmids from 16 fosA3-positive isolates were successfully transferred into the recipient bacteria by transformation or conjugation. In agreement with data determined with isolates from food animals, the IncHI2/ST3 and IncN-F33:A-:B-/F33:A-:B plasmids were the main vectors of fosA3 in E. coli. Additionally, F24:A-:B1, IncFIIK-IncR, IncFIIS, IncR, and two untypeable plasmids were found for the first time to be vectors for fosA3 in Enterobacterales. The genetic contexts of fosA3 in 15 Enterobacterales isolates differed due to insertion and/or loss of molecular modules mediated by mobile elements. However, all fosA3 genes were flanked by IS26, as commonly observed in other fosA3-carrying plasmids. Here, we report a high rate of detection of fosA3 genes, mediated by multiple plasmid vectors, in ESBL-producing Enterobacterales from retail vegetables. FosA3-producing Enterobacterales could be transmitted to the human body by direct contact or consumption of vegetables, which might pose a potential threat to public health. IMPORTANCE This report provides important information on the transmission and epidemiology of fosA3 among Enterobacterales isolates from vegetables. The rate of occurrence of fosA3 in ESBL-producing Enterobacterales from retail vegetables is high, and fosA3 was found to be carried by diverse plasmids. Some novel genetic contexts of fosA3 and novel fosA3-carrying plasmids, including several plasmid types common in K. pneumoniae, were identified, increasing the number of known transfer vectors for the fosA3 gene and reflecting the complexity of fosA3 transmission in Enterobacterales. The capture of fosA3 by the resident plasmid of K. pneumoniae will accelerate the spread of fosA3 in K. pneumoniae, one of the most pathogenic species in clinical medicine. Considering the clinical importance of fosfomycin, and the fact that vegetables are directly consumed, the fosfomycin resistance genes present a risk of transmission to the human body through the food chain and thus pose a threat to public health.
format	article
author	Luchao Lv Xiuyu Huang Jing Wang Ying Huang Xun Gao Yiyun Liu Qiaoli Zhou Qianhui Zhang Jun Yang Jian-Ying Guo Jian-Hua Liu
author_facet	Luchao Lv Xiuyu Huang Jing Wang Ying Huang Xun Gao Yiyun Liu Qiaoli Zhou Qianhui Zhang Jun Yang Jian-Ying Guo Jian-Hua Liu
author_sort	Luchao Lv
title	Multiple Plasmid Vectors Mediate the Spread of <italic toggle="yes">fosA3</italic> in Extended-Spectrum-β-Lactamase-Producing <italic toggle="yes">Enterobacterales</italic> Isolates from Retail Vegetables in China
title_short	Multiple Plasmid Vectors Mediate the Spread of <italic toggle="yes">fosA3</italic> in Extended-Spectrum-β-Lactamase-Producing <italic toggle="yes">Enterobacterales</italic> Isolates from Retail Vegetables in China
title_full	Multiple Plasmid Vectors Mediate the Spread of <italic toggle="yes">fosA3</italic> in Extended-Spectrum-β-Lactamase-Producing <italic toggle="yes">Enterobacterales</italic> Isolates from Retail Vegetables in China
title_fullStr	Multiple Plasmid Vectors Mediate the Spread of <italic toggle="yes">fosA3</italic> in Extended-Spectrum-β-Lactamase-Producing <italic toggle="yes">Enterobacterales</italic> Isolates from Retail Vegetables in China
title_full_unstemmed	Multiple Plasmid Vectors Mediate the Spread of <italic toggle="yes">fosA3</italic> in Extended-Spectrum-β-Lactamase-Producing <italic toggle="yes">Enterobacterales</italic> Isolates from Retail Vegetables in China
title_sort	multiple plasmid vectors mediate the spread of <italic toggle="yes">fosa3</italic> in extended-spectrum-β-lactamase-producing <italic toggle="yes">enterobacterales</italic> isolates from retail vegetables in china
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/df03be5b35134ec28e41a076adb1da9d
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Multiple Plasmid Vectors Mediate the Spread of <italic toggle="yes">fosA3</italic> in Extended-Spectrum-β-Lactamase-Producing <italic toggle="yes">Enterobacterales</italic> Isolates from Retail Vegetables in China

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