Genome sequencing and analysis of Talaromyces pinophilus provide insights into biotechnological applications

Abstract Species from the genus Talaromyces produce useful biomass-degrading enzymes and secondary metabolites. However, these enzymes and secondary metabolites are still poorly understood and have not been explored in depth because of a lack of comprehensive genetic information. Here, we report a 3...

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Autores principales:	Cheng-Xi Li, Shuai Zhao, Ting Zhang, Liang Xian, Lu-Sheng Liao, Jun-Liang Liu, Jia-Xun Feng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/df0bad6257e046949515de0d665761de
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spelling	oai:doaj.org-article:df0bad6257e046949515de0d665761de2021-12-02T16:06:52ZGenome sequencing and analysis of Talaromyces pinophilus provide insights into biotechnological applications10.1038/s41598-017-00567-02045-2322https://doaj.org/article/df0bad6257e046949515de0d665761de2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00567-0https://doaj.org/toc/2045-2322Abstract Species from the genus Talaromyces produce useful biomass-degrading enzymes and secondary metabolites. However, these enzymes and secondary metabolites are still poorly understood and have not been explored in depth because of a lack of comprehensive genetic information. Here, we report a 36.51-megabase genome assembly of Talaromyces pinophilus strain 1–95, with coverage of nine scaffolds of eight chromosomes with telomeric repeats at their ends and circular mitochondrial DNA. In total, 13,472 protein-coding genes were predicted. Of these, 803 were annotated to encode enzymes that act on carbohydrates, including 39 cellulose-degrading and 24 starch-degrading enzymes. In addition, 68 secondary metabolism gene clusters were identified, mainly including T1 polyketide synthase genes and nonribosomal peptide synthase genes. Comparative genomic analyses revealed that T. pinophilus 1–95 harbors more biomass-degrading enzymes and secondary metabolites than other related filamentous fungi. The prediction of the T. pinophilus 1–95 secretome indicated that approximately 50% of the biomass-degrading enzymes are secreted into the extracellular environment. These results expanded our genetic knowledge of the biomass-degrading enzyme system of T. pinophilus and its biosynthesis of secondary metabolites, facilitating the cultivation of T. pinophilus for high production of useful products.Cheng-Xi LiShuai ZhaoTing ZhangLiang XianLu-Sheng LiaoJun-Liang LiuJia-Xun FengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cheng-Xi Li Shuai Zhao Ting Zhang Liang Xian Lu-Sheng Liao Jun-Liang Liu Jia-Xun Feng Genome sequencing and analysis of Talaromyces pinophilus provide insights into biotechnological applications
description	Abstract Species from the genus Talaromyces produce useful biomass-degrading enzymes and secondary metabolites. However, these enzymes and secondary metabolites are still poorly understood and have not been explored in depth because of a lack of comprehensive genetic information. Here, we report a 36.51-megabase genome assembly of Talaromyces pinophilus strain 1–95, with coverage of nine scaffolds of eight chromosomes with telomeric repeats at their ends and circular mitochondrial DNA. In total, 13,472 protein-coding genes were predicted. Of these, 803 were annotated to encode enzymes that act on carbohydrates, including 39 cellulose-degrading and 24 starch-degrading enzymes. In addition, 68 secondary metabolism gene clusters were identified, mainly including T1 polyketide synthase genes and nonribosomal peptide synthase genes. Comparative genomic analyses revealed that T. pinophilus 1–95 harbors more biomass-degrading enzymes and secondary metabolites than other related filamentous fungi. The prediction of the T. pinophilus 1–95 secretome indicated that approximately 50% of the biomass-degrading enzymes are secreted into the extracellular environment. These results expanded our genetic knowledge of the biomass-degrading enzyme system of T. pinophilus and its biosynthesis of secondary metabolites, facilitating the cultivation of T. pinophilus for high production of useful products.
format	article
author	Cheng-Xi Li Shuai Zhao Ting Zhang Liang Xian Lu-Sheng Liao Jun-Liang Liu Jia-Xun Feng
author_facet	Cheng-Xi Li Shuai Zhao Ting Zhang Liang Xian Lu-Sheng Liao Jun-Liang Liu Jia-Xun Feng
author_sort	Cheng-Xi Li
title	Genome sequencing and analysis of Talaromyces pinophilus provide insights into biotechnological applications
title_short	Genome sequencing and analysis of Talaromyces pinophilus provide insights into biotechnological applications
title_full	Genome sequencing and analysis of Talaromyces pinophilus provide insights into biotechnological applications
title_fullStr	Genome sequencing and analysis of Talaromyces pinophilus provide insights into biotechnological applications
title_full_unstemmed	Genome sequencing and analysis of Talaromyces pinophilus provide insights into biotechnological applications
title_sort	genome sequencing and analysis of talaromyces pinophilus provide insights into biotechnological applications
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/df0bad6257e046949515de0d665761de
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Genome sequencing and analysis of Talaromyces pinophilus provide insights into biotechnological applications

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