Finding Nemo’s clock reveals switch from nocturnal to diurnal activity

Abstract Timing mechanisms play a key role in the biology of coral reef fish. Typically, fish larvae leave their reef after hatching, stay for a period in the open ocean before returning to the reef for settlement. During this dispersal, larvae use a time-compensated sun compass for orientation. How...

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Autores principales:	Gregor Schalm, Kristina Bruns, Nina Drachenberg, Nathalie Geyer, Nicholas S. Foulkes, Cristiano Bertolucci, Gabriele Gerlach
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/df1df7fb430947bb9d4d5bb16aa89e60
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spelling	oai:doaj.org-article:df1df7fb430947bb9d4d5bb16aa89e602021-12-02T11:45:03ZFinding Nemo’s clock reveals switch from nocturnal to diurnal activity10.1038/s41598-021-86244-92045-2322https://doaj.org/article/df1df7fb430947bb9d4d5bb16aa89e602021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86244-9https://doaj.org/toc/2045-2322Abstract Timing mechanisms play a key role in the biology of coral reef fish. Typically, fish larvae leave their reef after hatching, stay for a period in the open ocean before returning to the reef for settlement. During this dispersal, larvae use a time-compensated sun compass for orientation. However, the timing of settlement and how coral reef fish keep track of time via endogenous timing mechanisms is poorly understood. Here, we have studied the behavioural and genetic basis of diel rhythms in the clown anemonefish Amphiprion ocellaris. We document a behavioural shift from nocturnal larvae to diurnal adults, while juveniles show an intermediate pattern of activity which potentially indicates flexibility in the timing of settlement on a host anemone. qRTPCR analysis of six core circadian clock genes (bmal1, clocka, cry1b, per1b, per2, per3) reveals rhythmic gene expression patterns that are comparable in larvae and juveniles, and so do not reflect the corresponding activity changes. By establishing an embryonic cell line, we demonstrate that clown anemonefish possess an endogenous clock with similar properties to that of the zebrafish circadian clock. Furthermore, our study provides a first basis to study the multi-layered interaction of clocks from fish, anemones and their zooxanthellae endosymbionts.Gregor SchalmKristina BrunsNina DrachenbergNathalie GeyerNicholas S. FoulkesCristiano BertolucciGabriele GerlachNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gregor Schalm Kristina Bruns Nina Drachenberg Nathalie Geyer Nicholas S. Foulkes Cristiano Bertolucci Gabriele Gerlach Finding Nemo’s clock reveals switch from nocturnal to diurnal activity
description	Abstract Timing mechanisms play a key role in the biology of coral reef fish. Typically, fish larvae leave their reef after hatching, stay for a period in the open ocean before returning to the reef for settlement. During this dispersal, larvae use a time-compensated sun compass for orientation. However, the timing of settlement and how coral reef fish keep track of time via endogenous timing mechanisms is poorly understood. Here, we have studied the behavioural and genetic basis of diel rhythms in the clown anemonefish Amphiprion ocellaris. We document a behavioural shift from nocturnal larvae to diurnal adults, while juveniles show an intermediate pattern of activity which potentially indicates flexibility in the timing of settlement on a host anemone. qRTPCR analysis of six core circadian clock genes (bmal1, clocka, cry1b, per1b, per2, per3) reveals rhythmic gene expression patterns that are comparable in larvae and juveniles, and so do not reflect the corresponding activity changes. By establishing an embryonic cell line, we demonstrate that clown anemonefish possess an endogenous clock with similar properties to that of the zebrafish circadian clock. Furthermore, our study provides a first basis to study the multi-layered interaction of clocks from fish, anemones and their zooxanthellae endosymbionts.
format	article
author	Gregor Schalm Kristina Bruns Nina Drachenberg Nathalie Geyer Nicholas S. Foulkes Cristiano Bertolucci Gabriele Gerlach
author_facet	Gregor Schalm Kristina Bruns Nina Drachenberg Nathalie Geyer Nicholas S. Foulkes Cristiano Bertolucci Gabriele Gerlach
author_sort	Gregor Schalm
title	Finding Nemo’s clock reveals switch from nocturnal to diurnal activity
title_short	Finding Nemo’s clock reveals switch from nocturnal to diurnal activity
title_full	Finding Nemo’s clock reveals switch from nocturnal to diurnal activity
title_fullStr	Finding Nemo’s clock reveals switch from nocturnal to diurnal activity
title_full_unstemmed	Finding Nemo’s clock reveals switch from nocturnal to diurnal activity
title_sort	finding nemo’s clock reveals switch from nocturnal to diurnal activity
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/df1df7fb430947bb9d4d5bb16aa89e60
work_keys_str_mv	AT gregorschalm findingnemosclockrevealsswitchfromnocturnaltodiurnalactivity AT kristinabruns findingnemosclockrevealsswitchfromnocturnaltodiurnalactivity AT ninadrachenberg findingnemosclockrevealsswitchfromnocturnaltodiurnalactivity AT nathaliegeyer findingnemosclockrevealsswitchfromnocturnaltodiurnalactivity AT nicholassfoulkes findingnemosclockrevealsswitchfromnocturnaltodiurnalactivity AT cristianobertolucci findingnemosclockrevealsswitchfromnocturnaltodiurnalactivity AT gabrielegerlach findingnemosclockrevealsswitchfromnocturnaltodiurnalactivity
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Finding Nemo’s clock reveals switch from nocturnal to diurnal activity

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