Cellulolytic enzyme-producing thermophilic Actinobacteria isolated from the soil of Cisolok Geysers, West Java, Indonesia

Abstract. Setyaningsih PP, Ningsih F, Rachmania MK, Syafitri WA, Sari DCAF, Yabe S, Yokota A, Oetari A, Sjamsuridzal W. 2019. Cellulolytic enzyme-producing thermophilic Actinobacteria isolated from the soil of Cisolok Geysers, West Java, Indonesia. Biodiversitas 20: 3134-3141. This study investigate...

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Autores principales:	PUTRI PRATIWI SETYANINGSIH, FITRIA NINGSIH, MAZYTHA KINANTI RACHMANIA, WINDA AYU SYAFITRI, DHIAN CHITRA AYU FITRIA SARI, SHUHEI YABE, AKIRA YOKOTA, ARIYANTI OETARI, Wellyzar Sjamsuridzal
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	cellulase, cisolok geothermal area, thermophilic actinobacteria, soil Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/df2226a0108d4e39b66f3a1e8b211e7b
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spelling	oai:doaj.org-article:df2226a0108d4e39b66f3a1e8b211e7b2021-11-21T21:57:24ZCellulolytic enzyme-producing thermophilic Actinobacteria isolated from the soil of Cisolok Geysers, West Java, Indonesia1412-033X2085-472210.13057/biodiv/d201105https://doaj.org/article/df2226a0108d4e39b66f3a1e8b211e7b2019-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4289https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Setyaningsih PP, Ningsih F, Rachmania MK, Syafitri WA, Sari DCAF, Yabe S, Yokota A, Oetari A, Sjamsuridzal W. 2019. Cellulolytic enzyme-producing thermophilic Actinobacteria isolated from the soil of Cisolok Geysers, West Java, Indonesia. Biodiversitas 20: 3134-3141. This study investigated 17 thermophilic Actinobacteria isolated from the soil of geysers in the Cisolok geothermal area, West Java, as potential producers of cellulase. Screening for cellulase was performed on minimal (Mm) agar medium with and without the addition of 1% (w/v) carboxymethylcellulose (CMC) and microcrystalline cellulose (MCC), then incubated at 45, 50, 55 and 60°C for up to 7 days. Formation of clear zones around colonies indicated cellulose hydrolysis. The results showed that 15, 14, 4, and 3 isolates showed cellulolytic activity on CMC agar medium at 45, 50, 55, and 60°C, respectively, after 7 days of incubation. Three potential isolates showed cellulolytic activity on MCC agar medium after being incubated for 7 days at 45°C. Molecular identification based on the 16S rRNA gene was performed for three isolates with positive cellulolytic activity at 60°C. The results showed that the three isolates are closely related to Actinomadura keratinilytica WCC-2265T with 99.93-100% sequence similarities. A phylogenetic tree based on 16S rRNA gene sequences confirmed that the three isolates were clustered together with Actinomadura keratinilytica WCC-2265T with 100% bootstrap value. The tree also showed that cellulase producers and non-cellulase producers in Thermomonosporaceae are grouped into different clades.PUTRI PRATIWI SETYANINGSIHFITRIA NINGSIHMAZYTHA KINANTI RACHMANIAWINDA AYU SYAFITRIDHIAN CHITRA AYU FITRIA SARISHUHEI YABEAKIRA YOKOTAARIYANTI OETARIWellyzar SjamsuridzalMBI & UNS Soloarticlecellulase, cisolok geothermal area, thermophilic actinobacteria, soilBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	cellulase, cisolok geothermal area, thermophilic actinobacteria, soil Biology (General) QH301-705.5
spellingShingle	cellulase, cisolok geothermal area, thermophilic actinobacteria, soil Biology (General) QH301-705.5 PUTRI PRATIWI SETYANINGSIH FITRIA NINGSIH MAZYTHA KINANTI RACHMANIA WINDA AYU SYAFITRI DHIAN CHITRA AYU FITRIA SARI SHUHEI YABE AKIRA YOKOTA ARIYANTI OETARI Wellyzar Sjamsuridzal Cellulolytic enzyme-producing thermophilic Actinobacteria isolated from the soil of Cisolok Geysers, West Java, Indonesia
description	Abstract. Setyaningsih PP, Ningsih F, Rachmania MK, Syafitri WA, Sari DCAF, Yabe S, Yokota A, Oetari A, Sjamsuridzal W. 2019. Cellulolytic enzyme-producing thermophilic Actinobacteria isolated from the soil of Cisolok Geysers, West Java, Indonesia. Biodiversitas 20: 3134-3141. This study investigated 17 thermophilic Actinobacteria isolated from the soil of geysers in the Cisolok geothermal area, West Java, as potential producers of cellulase. Screening for cellulase was performed on minimal (Mm) agar medium with and without the addition of 1% (w/v) carboxymethylcellulose (CMC) and microcrystalline cellulose (MCC), then incubated at 45, 50, 55 and 60°C for up to 7 days. Formation of clear zones around colonies indicated cellulose hydrolysis. The results showed that 15, 14, 4, and 3 isolates showed cellulolytic activity on CMC agar medium at 45, 50, 55, and 60°C, respectively, after 7 days of incubation. Three potential isolates showed cellulolytic activity on MCC agar medium after being incubated for 7 days at 45°C. Molecular identification based on the 16S rRNA gene was performed for three isolates with positive cellulolytic activity at 60°C. The results showed that the three isolates are closely related to Actinomadura keratinilytica WCC-2265T with 99.93-100% sequence similarities. A phylogenetic tree based on 16S rRNA gene sequences confirmed that the three isolates were clustered together with Actinomadura keratinilytica WCC-2265T with 100% bootstrap value. The tree also showed that cellulase producers and non-cellulase producers in Thermomonosporaceae are grouped into different clades.
format	article
author	PUTRI PRATIWI SETYANINGSIH FITRIA NINGSIH MAZYTHA KINANTI RACHMANIA WINDA AYU SYAFITRI DHIAN CHITRA AYU FITRIA SARI SHUHEI YABE AKIRA YOKOTA ARIYANTI OETARI Wellyzar Sjamsuridzal
author_facet	PUTRI PRATIWI SETYANINGSIH FITRIA NINGSIH MAZYTHA KINANTI RACHMANIA WINDA AYU SYAFITRI DHIAN CHITRA AYU FITRIA SARI SHUHEI YABE AKIRA YOKOTA ARIYANTI OETARI Wellyzar Sjamsuridzal
author_sort	PUTRI PRATIWI SETYANINGSIH
title	Cellulolytic enzyme-producing thermophilic Actinobacteria isolated from the soil of Cisolok Geysers, West Java, Indonesia
title_short	Cellulolytic enzyme-producing thermophilic Actinobacteria isolated from the soil of Cisolok Geysers, West Java, Indonesia
title_full	Cellulolytic enzyme-producing thermophilic Actinobacteria isolated from the soil of Cisolok Geysers, West Java, Indonesia
title_fullStr	Cellulolytic enzyme-producing thermophilic Actinobacteria isolated from the soil of Cisolok Geysers, West Java, Indonesia
title_full_unstemmed	Cellulolytic enzyme-producing thermophilic Actinobacteria isolated from the soil of Cisolok Geysers, West Java, Indonesia
title_sort	cellulolytic enzyme-producing thermophilic actinobacteria isolated from the soil of cisolok geysers, west java, indonesia
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/df2226a0108d4e39b66f3a1e8b211e7b
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Cellulolytic enzyme-producing thermophilic Actinobacteria isolated from the soil of Cisolok Geysers, West Java, Indonesia

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