Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes

Hidayati NR, Suranto, Sajidan. 2018. Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes. Biodiversitas 19: 1683-1689. Aims of this research were to investigate the morphological character and isozyme banding patterns of Cucurbita moschata plants g...

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Autores principales:	NUR RAHMAH HIDAYATI, SURANTO SURANTO, SAJIDAN SAJIDAN
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	altitude cucurbita moschata isozyme morphology Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/df2a50e23ac8493f8a65b4de5f6ef107
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spelling	oai:doaj.org-article:df2a50e23ac8493f8a65b4de5f6ef1072021-11-16T14:04:05ZMorphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes1412-033X2085-472210.13057/biodiv/d190513https://doaj.org/article/df2a50e23ac8493f8a65b4de5f6ef1072018-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2930https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Hidayati NR, Suranto, Sajidan. 2018. Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes. Biodiversitas 19: 1683-1689. Aims of this research were to investigate the morphological character and isozyme banding patterns of Cucurbita moschata plants grown at three different altitudes. Samples in this study consisted of leaf, stem, and flowers. The morphological characters were conducted by direct observation in the field and analyzed descriptively as well as statically by one way ANOVA. The isozyme bands appearance of esterase and peroxidase of leaf samples were conducted using polyacrylamide gel electrophoresis (PAGE). Qualitative approach was used to analyze the presence and the absence of isozyme bands, while Retardation factor (Rf) was used to analyze quantitatively. The results showed that most plants grown at middle altitude (351-750 m asl.) were well-developed in terms of length of leaves, stems and flowers. Accordingly, the isozyme banding pattern of peroxidase was also found varied in plants grown at middle altitudes from which the presence of very unique bands was detected. Conversely, the band detected in plants grown at the lower and the highest altitudes was similar in term of band's number but it was different in the quality of the bands. Meanwhile, esterase isozyme banding pattern of plants grown at the lower and higher altitude had more bands than the middle altitude. Based on this result it is obvious that the isozyme data could be used to support in understanding the diversity morphological characters of plants grown in three different altitudes. This early result suggests that altitudes as a crucial factor in contributing the expression of isozyme appearance, which is useful for further pumpkin characterizations.NUR RAHMAH HIDAYATISURANTO SURANTOSAJIDAN SAJIDANMBI & UNS Soloarticlealtitudecucurbita moschataisozymemorphologyBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 5, Pp 1683-1689 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	altitude cucurbita moschata isozyme morphology Biology (General) QH301-705.5
spellingShingle	altitude cucurbita moschata isozyme morphology Biology (General) QH301-705.5 NUR RAHMAH HIDAYATI SURANTO SURANTO SAJIDAN SAJIDAN Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes
description	Hidayati NR, Suranto, Sajidan. 2018. Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes. Biodiversitas 19: 1683-1689. Aims of this research were to investigate the morphological character and isozyme banding patterns of Cucurbita moschata plants grown at three different altitudes. Samples in this study consisted of leaf, stem, and flowers. The morphological characters were conducted by direct observation in the field and analyzed descriptively as well as statically by one way ANOVA. The isozyme bands appearance of esterase and peroxidase of leaf samples were conducted using polyacrylamide gel electrophoresis (PAGE). Qualitative approach was used to analyze the presence and the absence of isozyme bands, while Retardation factor (Rf) was used to analyze quantitatively. The results showed that most plants grown at middle altitude (351-750 m asl.) were well-developed in terms of length of leaves, stems and flowers. Accordingly, the isozyme banding pattern of peroxidase was also found varied in plants grown at middle altitudes from which the presence of very unique bands was detected. Conversely, the band detected in plants grown at the lower and the highest altitudes was similar in term of band's number but it was different in the quality of the bands. Meanwhile, esterase isozyme banding pattern of plants grown at the lower and higher altitude had more bands than the middle altitude. Based on this result it is obvious that the isozyme data could be used to support in understanding the diversity morphological characters of plants grown in three different altitudes. This early result suggests that altitudes as a crucial factor in contributing the expression of isozyme appearance, which is useful for further pumpkin characterizations.
format	article
author	NUR RAHMAH HIDAYATI SURANTO SURANTO SAJIDAN SAJIDAN
author_facet	NUR RAHMAH HIDAYATI SURANTO SURANTO SAJIDAN SAJIDAN
author_sort	NUR RAHMAH HIDAYATI
title	Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes
title_short	Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes
title_full	Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes
title_fullStr	Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes
title_full_unstemmed	Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes
title_sort	morphological characteristics and isozyme banding patterns of cucurbita moschata at different altitudes
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/df2a50e23ac8493f8a65b4de5f6ef107
work_keys_str_mv	AT nurrahmahhidayati morphologicalcharacteristicsandisozymebandingpatternsofcucurbitamoschataatdifferentaltitudes AT surantosuranto morphologicalcharacteristicsandisozymebandingpatternsofcucurbitamoschataatdifferentaltitudes AT sajidansajidan morphologicalcharacteristicsandisozymebandingpatternsofcucurbitamoschataatdifferentaltitudes
_version_	1718426391963238400

Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes

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