SL2B aptamer and folic acid dual-targeting DNA nanostructures for synergic biological effect with chemotherapy to combat colorectal cancer

Pengchao Sun,* Nan Zhang,* Yafang Tang, Yanan Yang, Xiao Chu, Yongxing Zhao Department of Pharmaceutics, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, HeNan, People’s Republic of China *These authors contributed equally to the work Abstract: DNA nanostructures prep...

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Autores principales:	Sun P, Zhang N, Tang Y, Yang Y, Chu X, Zhao Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	DNA tetrahedron SL2B aptamer Doxorubicin colorectal cancer combined biological and chemotherapy VEGF/Notch signaling pathway Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/df2d11038dd44802a73c8137ece88f24
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spelling	oai:doaj.org-article:df2d11038dd44802a73c8137ece88f242021-12-02T01:01:50ZSL2B aptamer and folic acid dual-targeting DNA nanostructures for synergic biological effect with chemotherapy to combat colorectal cancer1178-2013https://doaj.org/article/df2d11038dd44802a73c8137ece88f242017-04-01T00:00:00Zhttps://www.dovepress.com/sl2b-aptamer-and-folic-acid-dual-targeting-dna-nanostructures-for-syne-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Pengchao Sun,* Nan Zhang,* Yafang Tang, Yanan Yang, Xiao Chu, Yongxing Zhao Department of Pharmaceutics, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, HeNan, People’s Republic of China *These authors contributed equally to the work Abstract: DNA nanostructures prepared by self-assembly possess good stability, high biocompatibility, and low immunogenicity as drug delivery vehicles. In this work, DNA tetrahedron (TD) was constructed and modified with SL2B aptamer (S) and folic acid (F). TD possessed a small diameter (~6 nm) and entered into the nucleus quickly. SL2B aptamer can inhibit cancer cell growth by disturbing vascular endothelial growth factor/Notch signaling pathways. To explore the effect of SL2B number on colorectal cancer inhibition, SL2B multimers (dimer, trimer, and tetramer) were constructed by functionalization of TD with different numbers of SL2B. One SL2B per TD was the most efficient anticancer strategy and showed significantly better anticancer efficacy than SL2B, probably due to the enhanced stability of SL2B by TD. Doxorubicin (DOX) is a potent anticancer agent that can intercalate into DNA double strands. Results showed that TD could facilitate DOX entrance into the nucleus and the intracellular delivery of DOX was further enhanced by functionalization of SL2B and F. DOX-intercalated TD modified with two F and two S (DOX@TD-2F2S) could cause sufficient HT-29 cell inhibition at a much lower DOX concentration. In sum, DOX@TD-2F2S exhibited a synergic anticancer biological effect with chemotherapy and can be a promising strategy for treating colorectal cancer. Keywords: SL2B aptamer, DNA tetrahedron, synergic biological effect with chemotherapy, colorectal cancer, doxorubicin, VEGF/Notch, folic acidSun PZhang NTang YYang YChu XZhao YDove Medical PressarticleDNA tetrahedronSL2B aptamerDoxorubicincolorectal cancercombined biological and chemotherapyVEGF/Notch signaling pathwayMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 2657-2672 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	DNA tetrahedron SL2B aptamer Doxorubicin colorectal cancer combined biological and chemotherapy VEGF/Notch signaling pathway Medicine (General) R5-920
spellingShingle	DNA tetrahedron SL2B aptamer Doxorubicin colorectal cancer combined biological and chemotherapy VEGF/Notch signaling pathway Medicine (General) R5-920 Sun P Zhang N Tang Y Yang Y Chu X Zhao Y SL2B aptamer and folic acid dual-targeting DNA nanostructures for synergic biological effect with chemotherapy to combat colorectal cancer
description	Pengchao Sun,* Nan Zhang,* Yafang Tang, Yanan Yang, Xiao Chu, Yongxing Zhao Department of Pharmaceutics, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, HeNan, People’s Republic of China *These authors contributed equally to the work Abstract: DNA nanostructures prepared by self-assembly possess good stability, high biocompatibility, and low immunogenicity as drug delivery vehicles. In this work, DNA tetrahedron (TD) was constructed and modified with SL2B aptamer (S) and folic acid (F). TD possessed a small diameter (~6 nm) and entered into the nucleus quickly. SL2B aptamer can inhibit cancer cell growth by disturbing vascular endothelial growth factor/Notch signaling pathways. To explore the effect of SL2B number on colorectal cancer inhibition, SL2B multimers (dimer, trimer, and tetramer) were constructed by functionalization of TD with different numbers of SL2B. One SL2B per TD was the most efficient anticancer strategy and showed significantly better anticancer efficacy than SL2B, probably due to the enhanced stability of SL2B by TD. Doxorubicin (DOX) is a potent anticancer agent that can intercalate into DNA double strands. Results showed that TD could facilitate DOX entrance into the nucleus and the intracellular delivery of DOX was further enhanced by functionalization of SL2B and F. DOX-intercalated TD modified with two F and two S (DOX@TD-2F2S) could cause sufficient HT-29 cell inhibition at a much lower DOX concentration. In sum, DOX@TD-2F2S exhibited a synergic anticancer biological effect with chemotherapy and can be a promising strategy for treating colorectal cancer. Keywords: SL2B aptamer, DNA tetrahedron, synergic biological effect with chemotherapy, colorectal cancer, doxorubicin, VEGF/Notch, folic acid
format	article
author	Sun P Zhang N Tang Y Yang Y Chu X Zhao Y
author_facet	Sun P Zhang N Tang Y Yang Y Chu X Zhao Y
author_sort	Sun P
title	SL2B aptamer and folic acid dual-targeting DNA nanostructures for synergic biological effect with chemotherapy to combat colorectal cancer
title_short	SL2B aptamer and folic acid dual-targeting DNA nanostructures for synergic biological effect with chemotherapy to combat colorectal cancer
title_full	SL2B aptamer and folic acid dual-targeting DNA nanostructures for synergic biological effect with chemotherapy to combat colorectal cancer
title_fullStr	SL2B aptamer and folic acid dual-targeting DNA nanostructures for synergic biological effect with chemotherapy to combat colorectal cancer
title_full_unstemmed	SL2B aptamer and folic acid dual-targeting DNA nanostructures for synergic biological effect with chemotherapy to combat colorectal cancer
title_sort	sl2b aptamer and folic acid dual-targeting dna nanostructures for synergic biological effect with chemotherapy to combat colorectal cancer
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/df2d11038dd44802a73c8137ece88f24
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SL2B aptamer and folic acid dual-targeting DNA nanostructures for synergic biological effect with chemotherapy to combat colorectal cancer

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