Whole mitochondrial genome sequencing highlights mitochondrial impact in gastric cancer

Abstract Mitochondria are organelles that perform major roles in cellular operation. Thus, alterations in mitochondrial genome (mtGenome) may lead to mitochondrial dysfunction and cellular deregulation, influencing carcinogenesis. Gastric cancer (GC) is one of the most incident and mortal types of c...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Giovanna Chaves Cavalcante, Anderson N. R. Marinho, Ana Karyssa Anaissi, Tatiana Vinasco-Sandoval, André Ribeiro-dos-Santos, Amanda Ferreira Vidal, Gilderlanio S. de Araújo, Samia Demachki, Ândrea Ribeiro-dos-Santos
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/df2df62ae1ba4182bdb4cf847bb6e43b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:df2df62ae1ba4182bdb4cf847bb6e43b
record_format	dspace
spelling	oai:doaj.org-article:df2df62ae1ba4182bdb4cf847bb6e43b2021-12-02T15:08:32ZWhole mitochondrial genome sequencing highlights mitochondrial impact in gastric cancer10.1038/s41598-019-51951-x2045-2322https://doaj.org/article/df2df62ae1ba4182bdb4cf847bb6e43b2019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-51951-xhttps://doaj.org/toc/2045-2322Abstract Mitochondria are organelles that perform major roles in cellular operation. Thus, alterations in mitochondrial genome (mtGenome) may lead to mitochondrial dysfunction and cellular deregulation, influencing carcinogenesis. Gastric cancer (GC) is one of the most incident and mortal types of cancer in Brazil, particularly in the Amazon region. Here, we sequenced and compared the whole mtGenome extracted from FFPE tissue samples of GC patients (tumor and internal control – IC) and cancer-free individuals (external control – EC) from this region. We found 3-fold more variants and up to 9-fold more heteroplasmic regions in tumor when compared to paired IC samples. Moreover, tumor presented more heteroplasmic variants when compared to EC, while IC and EC showed no significant difference when compared to each other. Tumor also presented substantially more variants in the following regions: MT-RNR1, MT-ND5, MT-ND4, MT-ND2, MT-DLOOP1 and MT-CO1. In addition, our haplogroup results indicate an association of Native American ancestry (particularly haplogroup C) to gastric cancer development. To the best of our knowledge, this is the first study to sequence the whole mtGenome from FFPE samples and to apply mtGenome analysis in association to GC in Brazil.Giovanna Chaves CavalcanteAnderson N. R. MarinhoAna Karyssa AnaissiTatiana Vinasco-SandovalAndré Ribeiro-dos-SantosAmanda Ferreira VidalGilderlanio S. de AraújoSamia DemachkiÂndrea Ribeiro-dos-SantosNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Giovanna Chaves Cavalcante Anderson N. R. Marinho Ana Karyssa Anaissi Tatiana Vinasco-Sandoval André Ribeiro-dos-Santos Amanda Ferreira Vidal Gilderlanio S. de Araújo Samia Demachki Ândrea Ribeiro-dos-Santos Whole mitochondrial genome sequencing highlights mitochondrial impact in gastric cancer
description	Abstract Mitochondria are organelles that perform major roles in cellular operation. Thus, alterations in mitochondrial genome (mtGenome) may lead to mitochondrial dysfunction and cellular deregulation, influencing carcinogenesis. Gastric cancer (GC) is one of the most incident and mortal types of cancer in Brazil, particularly in the Amazon region. Here, we sequenced and compared the whole mtGenome extracted from FFPE tissue samples of GC patients (tumor and internal control – IC) and cancer-free individuals (external control – EC) from this region. We found 3-fold more variants and up to 9-fold more heteroplasmic regions in tumor when compared to paired IC samples. Moreover, tumor presented more heteroplasmic variants when compared to EC, while IC and EC showed no significant difference when compared to each other. Tumor also presented substantially more variants in the following regions: MT-RNR1, MT-ND5, MT-ND4, MT-ND2, MT-DLOOP1 and MT-CO1. In addition, our haplogroup results indicate an association of Native American ancestry (particularly haplogroup C) to gastric cancer development. To the best of our knowledge, this is the first study to sequence the whole mtGenome from FFPE samples and to apply mtGenome analysis in association to GC in Brazil.
format	article
author	Giovanna Chaves Cavalcante Anderson N. R. Marinho Ana Karyssa Anaissi Tatiana Vinasco-Sandoval André Ribeiro-dos-Santos Amanda Ferreira Vidal Gilderlanio S. de Araújo Samia Demachki Ândrea Ribeiro-dos-Santos
author_facet	Giovanna Chaves Cavalcante Anderson N. R. Marinho Ana Karyssa Anaissi Tatiana Vinasco-Sandoval André Ribeiro-dos-Santos Amanda Ferreira Vidal Gilderlanio S. de Araújo Samia Demachki Ândrea Ribeiro-dos-Santos
author_sort	Giovanna Chaves Cavalcante
title	Whole mitochondrial genome sequencing highlights mitochondrial impact in gastric cancer
title_short	Whole mitochondrial genome sequencing highlights mitochondrial impact in gastric cancer
title_full	Whole mitochondrial genome sequencing highlights mitochondrial impact in gastric cancer
title_fullStr	Whole mitochondrial genome sequencing highlights mitochondrial impact in gastric cancer
title_full_unstemmed	Whole mitochondrial genome sequencing highlights mitochondrial impact in gastric cancer
title_sort	whole mitochondrial genome sequencing highlights mitochondrial impact in gastric cancer
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/df2df62ae1ba4182bdb4cf847bb6e43b
work_keys_str_mv	AT giovannachavescavalcante wholemitochondrialgenomesequencinghighlightsmitochondrialimpactingastriccancer AT andersonnrmarinho wholemitochondrialgenomesequencinghighlightsmitochondrialimpactingastriccancer AT anakaryssaanaissi wholemitochondrialgenomesequencinghighlightsmitochondrialimpactingastriccancer AT tatianavinascosandoval wholemitochondrialgenomesequencinghighlightsmitochondrialimpactingastriccancer AT andreribeirodossantos wholemitochondrialgenomesequencinghighlightsmitochondrialimpactingastriccancer AT amandaferreiravidal wholemitochondrialgenomesequencinghighlightsmitochondrialimpactingastriccancer AT gilderlaniosdearaujo wholemitochondrialgenomesequencinghighlightsmitochondrialimpactingastriccancer AT samiademachki wholemitochondrialgenomesequencinghighlightsmitochondrialimpactingastriccancer AT andrearibeirodossantos wholemitochondrialgenomesequencinghighlightsmitochondrialimpactingastriccancer
_version_	1718388094442405888

Whole mitochondrial genome sequencing highlights mitochondrial impact in gastric cancer

Ejemplares similares