Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study

Health monitoring during offshore saturation diving is complicated due to restricted access to the divers, the desire to keep invasive procedures to a minimum, and limited opportunity for laboratory work onboard dive support vessels (DSV). In this pilot study, we examined whether measuring salivary...

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Autores principales:	Roxane Monnoyer, Jacky Lautridou, Sanjoy Deb, Astrid Hjelde, Ingrid Eftedal
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	saliva biomarkers cytokine inflammation hyperbaric heliox saturation physiological stress Physiology QP1-981
Acceso en línea:	https://doaj.org/article/df364293477b451b9a8189a8c5095c68
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spelling	oai:doaj.org-article:df364293477b451b9a8189a8c5095c682021-12-01T18:16:35ZUsing Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study1664-042X10.3389/fphys.2021.791525https://doaj.org/article/df364293477b451b9a8189a8c5095c682021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphys.2021.791525/fullhttps://doaj.org/toc/1664-042XHealth monitoring during offshore saturation diving is complicated due to restricted access to the divers, the desire to keep invasive procedures to a minimum, and limited opportunity for laboratory work onboard dive support vessels (DSV). In this pilot study, we examined whether measuring salivary biomarkrers in samples collected by the divers themselves might be a feasible approach to environmental stress assessment. Nine saturation divers were trained in the passive drool method for saliva collection and proceeded to collect samples at nine time points before, during, and after an offshore commercial saturation diving campaign. Samples collected within the hyperbaric living chambers were decompressed and stored frozen at −20°C onboard the DSV until they were shipped to land for analysis. Passive drool samples were collected without loss and assayed for a selection of salivary biomarkers: secretory immunoglobulin A (SIgA), C-reactive protein (CRP), tumor necrosis factor (TNF)-α, interleukins IL-6, IL-8, IL-1β, as well as cortisol and alpha-amylase. During the bottom phase of the hyperbaric saturation, SIgA, CRP, TNF-α, IL-8 and IL-1β increased significantly, whereas IL-6, cortisol and alpha-amylase were unchanged. All markers returned to pre-dive levels after the divers were decompressed back to surface pressure. We conclude that salivary biomarker analysis may be a feasible approach to stress assessment in offshore saturation diving. The results of our pilot test are consonant with an activation of the sympathetic nervous system related to systemic inflammation during hyperbaric and hyperoxic saturation.Roxane MonnoyerJacky LautridouSanjoy DebSanjoy DebAstrid HjeldeIngrid EftedalIngrid EftedalFrontiers Media S.A.articlesalivabiomarkerscytokineinflammationhyperbaric heliox saturationphysiological stressPhysiologyQP1-981ENFrontiers in Physiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	saliva biomarkers cytokine inflammation hyperbaric heliox saturation physiological stress Physiology QP1-981
spellingShingle	saliva biomarkers cytokine inflammation hyperbaric heliox saturation physiological stress Physiology QP1-981 Roxane Monnoyer Jacky Lautridou Sanjoy Deb Sanjoy Deb Astrid Hjelde Ingrid Eftedal Ingrid Eftedal Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study
description	Health monitoring during offshore saturation diving is complicated due to restricted access to the divers, the desire to keep invasive procedures to a minimum, and limited opportunity for laboratory work onboard dive support vessels (DSV). In this pilot study, we examined whether measuring salivary biomarkrers in samples collected by the divers themselves might be a feasible approach to environmental stress assessment. Nine saturation divers were trained in the passive drool method for saliva collection and proceeded to collect samples at nine time points before, during, and after an offshore commercial saturation diving campaign. Samples collected within the hyperbaric living chambers were decompressed and stored frozen at −20°C onboard the DSV until they were shipped to land for analysis. Passive drool samples were collected without loss and assayed for a selection of salivary biomarkers: secretory immunoglobulin A (SIgA), C-reactive protein (CRP), tumor necrosis factor (TNF)-α, interleukins IL-6, IL-8, IL-1β, as well as cortisol and alpha-amylase. During the bottom phase of the hyperbaric saturation, SIgA, CRP, TNF-α, IL-8 and IL-1β increased significantly, whereas IL-6, cortisol and alpha-amylase were unchanged. All markers returned to pre-dive levels after the divers were decompressed back to surface pressure. We conclude that salivary biomarker analysis may be a feasible approach to stress assessment in offshore saturation diving. The results of our pilot test are consonant with an activation of the sympathetic nervous system related to systemic inflammation during hyperbaric and hyperoxic saturation.
format	article
author	Roxane Monnoyer Jacky Lautridou Sanjoy Deb Sanjoy Deb Astrid Hjelde Ingrid Eftedal Ingrid Eftedal
author_facet	Roxane Monnoyer Jacky Lautridou Sanjoy Deb Sanjoy Deb Astrid Hjelde Ingrid Eftedal Ingrid Eftedal
author_sort	Roxane Monnoyer
title	Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study
title_short	Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study
title_full	Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study
title_fullStr	Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study
title_full_unstemmed	Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study
title_sort	using salivary biomarkers for stress assessment in offshore saturation diving: a pilot study
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/df364293477b451b9a8189a8c5095c68
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Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study

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