High-Resolution Magnetic Resonance Imaging of the Regenerating Adult Zebrafish Heart

Abstract The adult zebrafish is a well-established model for studying heart regeneration, but due to its tissue opaqueness, repair has been primarily assessed using destructive histology, precluding repeated investigations of the same animal. We present a high-resolution, non-invasive in vivo magnet...

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Autores principales:	Jana Koth, Mahon L. Maguire, Darryl McClymont, Leonie Diffley, Victoria L. Thornton, John Beech, Roger K. Patient, Paul R. Riley, Jürgen E. Schneider
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/df3a001c45664798981cda1674ac34b4
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spelling	oai:doaj.org-article:df3a001c45664798981cda1674ac34b42021-12-02T16:06:30ZHigh-Resolution Magnetic Resonance Imaging of the Regenerating Adult Zebrafish Heart10.1038/s41598-017-03050-y2045-2322https://doaj.org/article/df3a001c45664798981cda1674ac34b42017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03050-yhttps://doaj.org/toc/2045-2322Abstract The adult zebrafish is a well-established model for studying heart regeneration, but due to its tissue opaqueness, repair has been primarily assessed using destructive histology, precluding repeated investigations of the same animal. We present a high-resolution, non-invasive in vivo magnetic resonance imaging (MRI) method incorporating a miniature respiratory and anaesthetic perfusion set-up for live adult zebrafish, allowing for visualization of scar formation and heart regeneration in the same animal over time at an isotropic 31 µm voxel resolution. To test the method, we compared well and poorly healing cardiac ventricles using a transgenic fish model that exhibits heat-shock (HS) inducible impaired heart regeneration. HS-treated groups revealed persistent scar tissue for 10 weeks, while control groups were healed after 4 weeks. Application of the advanced MRI technique allowed clear discrimination of levels of repair following cryo- and resection injury for several months. It further provides a novel tool for in vivo time-lapse imaging of adult fish for non-cardiac studies, as the method can be readily applied to image wound healing in other injured or diseased tissues, or to monitor tissue changes over time, thus expanding the range of questions that can be addressed in adult zebrafish and other small aquatic species.Jana KothMahon L. MaguireDarryl McClymontLeonie DiffleyVictoria L. ThorntonJohn BeechRoger K. PatientPaul R. RileyJürgen E. SchneiderNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jana Koth Mahon L. Maguire Darryl McClymont Leonie Diffley Victoria L. Thornton John Beech Roger K. Patient Paul R. Riley Jürgen E. Schneider High-Resolution Magnetic Resonance Imaging of the Regenerating Adult Zebrafish Heart
description	Abstract The adult zebrafish is a well-established model for studying heart regeneration, but due to its tissue opaqueness, repair has been primarily assessed using destructive histology, precluding repeated investigations of the same animal. We present a high-resolution, non-invasive in vivo magnetic resonance imaging (MRI) method incorporating a miniature respiratory and anaesthetic perfusion set-up for live adult zebrafish, allowing for visualization of scar formation and heart regeneration in the same animal over time at an isotropic 31 µm voxel resolution. To test the method, we compared well and poorly healing cardiac ventricles using a transgenic fish model that exhibits heat-shock (HS) inducible impaired heart regeneration. HS-treated groups revealed persistent scar tissue for 10 weeks, while control groups were healed after 4 weeks. Application of the advanced MRI technique allowed clear discrimination of levels of repair following cryo- and resection injury for several months. It further provides a novel tool for in vivo time-lapse imaging of adult fish for non-cardiac studies, as the method can be readily applied to image wound healing in other injured or diseased tissues, or to monitor tissue changes over time, thus expanding the range of questions that can be addressed in adult zebrafish and other small aquatic species.
format	article
author	Jana Koth Mahon L. Maguire Darryl McClymont Leonie Diffley Victoria L. Thornton John Beech Roger K. Patient Paul R. Riley Jürgen E. Schneider
author_facet	Jana Koth Mahon L. Maguire Darryl McClymont Leonie Diffley Victoria L. Thornton John Beech Roger K. Patient Paul R. Riley Jürgen E. Schneider
author_sort	Jana Koth
title	High-Resolution Magnetic Resonance Imaging of the Regenerating Adult Zebrafish Heart
title_short	High-Resolution Magnetic Resonance Imaging of the Regenerating Adult Zebrafish Heart
title_full	High-Resolution Magnetic Resonance Imaging of the Regenerating Adult Zebrafish Heart
title_fullStr	High-Resolution Magnetic Resonance Imaging of the Regenerating Adult Zebrafish Heart
title_full_unstemmed	High-Resolution Magnetic Resonance Imaging of the Regenerating Adult Zebrafish Heart
title_sort	high-resolution magnetic resonance imaging of the regenerating adult zebrafish heart
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/df3a001c45664798981cda1674ac34b4
work_keys_str_mv	AT janakoth highresolutionmagneticresonanceimagingoftheregeneratingadultzebrafishheart AT mahonlmaguire highresolutionmagneticresonanceimagingoftheregeneratingadultzebrafishheart AT darrylmcclymont highresolutionmagneticresonanceimagingoftheregeneratingadultzebrafishheart AT leoniediffley highresolutionmagneticresonanceimagingoftheregeneratingadultzebrafishheart AT victorialthornton highresolutionmagneticresonanceimagingoftheregeneratingadultzebrafishheart AT johnbeech highresolutionmagneticresonanceimagingoftheregeneratingadultzebrafishheart AT rogerkpatient highresolutionmagneticresonanceimagingoftheregeneratingadultzebrafishheart AT paulrriley highresolutionmagneticresonanceimagingoftheregeneratingadultzebrafishheart AT jurgeneschneider highresolutionmagneticresonanceimagingoftheregeneratingadultzebrafishheart
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High-Resolution Magnetic Resonance Imaging of the Regenerating Adult Zebrafish Heart

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