Microfluidic platform accelerates tissue processing into single cells for molecular analysis and primary culture models

Existing methods for tissue dissociation are inefficient and lead to variable outcomes and biases. Here, the authors present a microfluidic platform that combines digestion, disaggregation and filtration of tissue to allow single cell analysis and RNA sequencing.

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Detalles Bibliográficos
Autores principales: Jeremy A. Lombardo, Marzieh Aliaghaei, Quy H. Nguyen, Kai Kessenbrock, Jered B. Haun
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/df431d77a95e4931b3e810d6dee41623
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Descripción
Sumario:Existing methods for tissue dissociation are inefficient and lead to variable outcomes and biases. Here, the authors present a microfluidic platform that combines digestion, disaggregation and filtration of tissue to allow single cell analysis and RNA sequencing.