Microfluidic platform accelerates tissue processing into single cells for molecular analysis and primary culture models

Existing methods for tissue dissociation are inefficient and lead to variable outcomes and biases. Here, the authors present a microfluidic platform that combines digestion, disaggregation and filtration of tissue to allow single cell analysis and RNA sequencing.

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Autores principales:	Jeremy A. Lombardo, Marzieh Aliaghaei, Quy H. Nguyen, Kai Kessenbrock, Jered B. Haun
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/df431d77a95e4931b3e810d6dee41623
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spelling	oai:doaj.org-article:df431d77a95e4931b3e810d6dee416232021-12-02T16:51:34ZMicrofluidic platform accelerates tissue processing into single cells for molecular analysis and primary culture models10.1038/s41467-021-23238-12041-1723https://doaj.org/article/df431d77a95e4931b3e810d6dee416232021-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-23238-1https://doaj.org/toc/2041-1723Existing methods for tissue dissociation are inefficient and lead to variable outcomes and biases. Here, the authors present a microfluidic platform that combines digestion, disaggregation and filtration of tissue to allow single cell analysis and RNA sequencing.Jeremy A. LombardoMarzieh AliaghaeiQuy H. NguyenKai KessenbrockJered B. HaunNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jeremy A. Lombardo Marzieh Aliaghaei Quy H. Nguyen Kai Kessenbrock Jered B. Haun Microfluidic platform accelerates tissue processing into single cells for molecular analysis and primary culture models
description	Existing methods for tissue dissociation are inefficient and lead to variable outcomes and biases. Here, the authors present a microfluidic platform that combines digestion, disaggregation and filtration of tissue to allow single cell analysis and RNA sequencing.
format	article
author	Jeremy A. Lombardo Marzieh Aliaghaei Quy H. Nguyen Kai Kessenbrock Jered B. Haun
author_facet	Jeremy A. Lombardo Marzieh Aliaghaei Quy H. Nguyen Kai Kessenbrock Jered B. Haun
author_sort	Jeremy A. Lombardo
title	Microfluidic platform accelerates tissue processing into single cells for molecular analysis and primary culture models
title_short	Microfluidic platform accelerates tissue processing into single cells for molecular analysis and primary culture models
title_full	Microfluidic platform accelerates tissue processing into single cells for molecular analysis and primary culture models
title_fullStr	Microfluidic platform accelerates tissue processing into single cells for molecular analysis and primary culture models
title_full_unstemmed	Microfluidic platform accelerates tissue processing into single cells for molecular analysis and primary culture models
title_sort	microfluidic platform accelerates tissue processing into single cells for molecular analysis and primary culture models
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/df431d77a95e4931b3e810d6dee41623
work_keys_str_mv	AT jeremyalombardo microfluidicplatformacceleratestissueprocessingintosinglecellsformolecularanalysisandprimaryculturemodels AT marziehaliaghaei microfluidicplatformacceleratestissueprocessingintosinglecellsformolecularanalysisandprimaryculturemodels AT quyhnguyen microfluidicplatformacceleratestissueprocessingintosinglecellsformolecularanalysisandprimaryculturemodels AT kaikessenbrock microfluidicplatformacceleratestissueprocessingintosinglecellsformolecularanalysisandprimaryculturemodels AT jeredbhaun microfluidicplatformacceleratestissueprocessingintosinglecellsformolecularanalysisandprimaryculturemodels
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Microfluidic platform accelerates tissue processing into single cells for molecular analysis and primary culture models

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