Comparison of DNA-hydrolyzing antibodies from the cerebrospinal fluid and serum of patients with multiple sclerosis.

It was found that high-affinity anti-DNA antibodies were one of the major components of the intrathecal IgG response in multiple sclerosis (MS) patients [Williamson et al., PNAS, 2001]. Recently we have shown that IgGs from the sera of MS patients are active in the hydrolysis of DNA. Here we have sh...

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Autores principales:	Taisiya A Parkhomenko, Vasilii B Doronin, Massimiliano Castellazzi, Marina Padroni, Michela Pastore, Valentina N Buneva, Enrico Granieri, Georgy A Nevinsky
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/df4745b2f4b24fe2adebe74af7ecccf3
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spelling	oai:doaj.org-article:df4745b2f4b24fe2adebe74af7ecccf32021-11-18T08:23:05ZComparison of DNA-hydrolyzing antibodies from the cerebrospinal fluid and serum of patients with multiple sclerosis.1932-620310.1371/journal.pone.0093001https://doaj.org/article/df4745b2f4b24fe2adebe74af7ecccf32014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24736683/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203It was found that high-affinity anti-DNA antibodies were one of the major components of the intrathecal IgG response in multiple sclerosis (MS) patients [Williamson et al., PNAS, 2001]. Recently we have shown that IgGs from the sera of MS patients are active in the hydrolysis of DNA. Here we have shown, for the first time, that average concentration of total proteins (132-fold), total IgGs (194-fold) and anti-DNA antibodies (200-fold) in the sera is significantly higher than that in the cerebrospinal fluid (CSF) of fifteen MS patients. The relative activities of total protein from sera and CSFs varied remarkably from patient to patient. It was surprising that the specific DNase activity of the total protein of CSF reparations were 198-fold higher than the serum ones. Electrophoretically and immunologically homogeneous IgGs were obtained by sequential affinity chromatography of the CSF proteins on protein G-Sepharose and FPLC gel filtration. We present first evidence showing that IgGs from CSF not only bind but efficiently hydrolyze DNA and that average specific DNase activity of homogeneous antibodies from CSF is unpredictably ∼49-fold higher than that from the sera of the same MS patients. Some possible reasons of these findings are discussed. We suggest that DNase IgGs of CSF may promote important neuropathologic mechanisms in this chronic inflammatory disorder and MS pathogenesis development.Taisiya A ParkhomenkoVasilii B DoroninMassimiliano CastellazziMarina PadroniMichela PastoreValentina N BunevaEnrico GranieriGeorgy A NevinskyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 4, p e93001 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Taisiya A Parkhomenko Vasilii B Doronin Massimiliano Castellazzi Marina Padroni Michela Pastore Valentina N Buneva Enrico Granieri Georgy A Nevinsky Comparison of DNA-hydrolyzing antibodies from the cerebrospinal fluid and serum of patients with multiple sclerosis.
description	It was found that high-affinity anti-DNA antibodies were one of the major components of the intrathecal IgG response in multiple sclerosis (MS) patients [Williamson et al., PNAS, 2001]. Recently we have shown that IgGs from the sera of MS patients are active in the hydrolysis of DNA. Here we have shown, for the first time, that average concentration of total proteins (132-fold), total IgGs (194-fold) and anti-DNA antibodies (200-fold) in the sera is significantly higher than that in the cerebrospinal fluid (CSF) of fifteen MS patients. The relative activities of total protein from sera and CSFs varied remarkably from patient to patient. It was surprising that the specific DNase activity of the total protein of CSF reparations were 198-fold higher than the serum ones. Electrophoretically and immunologically homogeneous IgGs were obtained by sequential affinity chromatography of the CSF proteins on protein G-Sepharose and FPLC gel filtration. We present first evidence showing that IgGs from CSF not only bind but efficiently hydrolyze DNA and that average specific DNase activity of homogeneous antibodies from CSF is unpredictably ∼49-fold higher than that from the sera of the same MS patients. Some possible reasons of these findings are discussed. We suggest that DNase IgGs of CSF may promote important neuropathologic mechanisms in this chronic inflammatory disorder and MS pathogenesis development.
format	article
author	Taisiya A Parkhomenko Vasilii B Doronin Massimiliano Castellazzi Marina Padroni Michela Pastore Valentina N Buneva Enrico Granieri Georgy A Nevinsky
author_facet	Taisiya A Parkhomenko Vasilii B Doronin Massimiliano Castellazzi Marina Padroni Michela Pastore Valentina N Buneva Enrico Granieri Georgy A Nevinsky
author_sort	Taisiya A Parkhomenko
title	Comparison of DNA-hydrolyzing antibodies from the cerebrospinal fluid and serum of patients with multiple sclerosis.
title_short	Comparison of DNA-hydrolyzing antibodies from the cerebrospinal fluid and serum of patients with multiple sclerosis.
title_full	Comparison of DNA-hydrolyzing antibodies from the cerebrospinal fluid and serum of patients with multiple sclerosis.
title_fullStr	Comparison of DNA-hydrolyzing antibodies from the cerebrospinal fluid and serum of patients with multiple sclerosis.
title_full_unstemmed	Comparison of DNA-hydrolyzing antibodies from the cerebrospinal fluid and serum of patients with multiple sclerosis.
title_sort	comparison of dna-hydrolyzing antibodies from the cerebrospinal fluid and serum of patients with multiple sclerosis.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/df4745b2f4b24fe2adebe74af7ecccf3
work_keys_str_mv	AT taisiyaaparkhomenko comparisonofdnahydrolyzingantibodiesfromthecerebrospinalfluidandserumofpatientswithmultiplesclerosis AT vasiliibdoronin comparisonofdnahydrolyzingantibodiesfromthecerebrospinalfluidandserumofpatientswithmultiplesclerosis AT massimilianocastellazzi comparisonofdnahydrolyzingantibodiesfromthecerebrospinalfluidandserumofpatientswithmultiplesclerosis AT marinapadroni comparisonofdnahydrolyzingantibodiesfromthecerebrospinalfluidandserumofpatientswithmultiplesclerosis AT michelapastore comparisonofdnahydrolyzingantibodiesfromthecerebrospinalfluidandserumofpatientswithmultiplesclerosis AT valentinanbuneva comparisonofdnahydrolyzingantibodiesfromthecerebrospinalfluidandserumofpatientswithmultiplesclerosis AT enricogranieri comparisonofdnahydrolyzingantibodiesfromthecerebrospinalfluidandserumofpatientswithmultiplesclerosis AT georgyanevinsky comparisonofdnahydrolyzingantibodiesfromthecerebrospinalfluidandserumofpatientswithmultiplesclerosis
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Comparison of DNA-hydrolyzing antibodies from the cerebrospinal fluid and serum of patients with multiple sclerosis.

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