Potential Vasculoprotective Effects of Blackcurrant (<i>Ribes nigrum</i>) Extract in Diabetic KK-A<sup>y</sup> Mice

Polyphenols are bioactive compounds found naturally in fruits and vegetables; they are widely used in disease prevention and health maintenance. Polyphenol-rich blackcurrant extract (BCE) exerts beneficial effects on vascular health in menopausal model animals. However, the vasculoprotective effects...

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Autores principales:	Kayo Horie, Hayato Maeda, Naoki Nanashima, Indrawati Oey
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	polyphenol blackcurrant extract diabetes model animal endothelial nitric oxide (eNOS) alpha-smooth muscle actin (α-SMA) Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/df47a5eafec5490d801833d67dc293dd
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spelling	oai:doaj.org-article:df47a5eafec5490d801833d67dc293dd2021-11-11T18:28:09ZPotential Vasculoprotective Effects of Blackcurrant (<i>Ribes nigrum</i>) Extract in Diabetic KK-A<sup>y</sup> Mice10.3390/molecules262164591420-3049https://doaj.org/article/df47a5eafec5490d801833d67dc293dd2021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6459https://doaj.org/toc/1420-3049Polyphenols are bioactive compounds found naturally in fruits and vegetables; they are widely used in disease prevention and health maintenance. Polyphenol-rich blackcurrant extract (BCE) exerts beneficial effects on vascular health in menopausal model animals. However, the vasculoprotective effects in diabetes mellitus (DM) and atherosclerotic vascular disease secondary to DM are unknown. Therefore, we investigated whether BCE is effective in preventing atherosclerosis using KK-A<sup>y</sup> mice as a diabetes model. The mice were divided into three groups and fed a high-fat diet supplemented with 1% BCE (BCE1), 3% BCE (BCE2), or Control for 9 weeks. The mice in the BCE2 group showed a considerable reduction in the disturbance of elastic lamina, foam cell formation, and vascular remodeling compared to those in the BCE1 and Control groups. Immunohistochemical staining indicated that the score of endothelial nitric oxide synthase staining intensity was significantly higher in both BCE2 (2.9) and BCE1 (1.9) compared to that in the Control (1.1). Furthermore, the score for the percentage of alpha-smooth muscle actin was significantly lower in the BCE2 (2.9%) than in the Control (2.1%). Our results suggest that the intake of anthocyanin-rich BCE could have beneficial effects on the blood vessels of diabetic patients.Kayo HorieHayato MaedaNaoki NanashimaIndrawati OeyMDPI AGarticlepolyphenolblackcurrant extractdiabetes model animalendothelial nitric oxide (eNOS)alpha-smooth muscle actin (α-SMA)Organic chemistryQD241-441ENMolecules, Vol 26, Iss 6459, p 6459 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	polyphenol blackcurrant extract diabetes model animal endothelial nitric oxide (eNOS) alpha-smooth muscle actin (α-SMA) Organic chemistry QD241-441
spellingShingle	polyphenol blackcurrant extract diabetes model animal endothelial nitric oxide (eNOS) alpha-smooth muscle actin (α-SMA) Organic chemistry QD241-441 Kayo Horie Hayato Maeda Naoki Nanashima Indrawati Oey Potential Vasculoprotective Effects of Blackcurrant (<i>Ribes nigrum</i>) Extract in Diabetic KK-A<sup>y</sup> Mice
description	Polyphenols are bioactive compounds found naturally in fruits and vegetables; they are widely used in disease prevention and health maintenance. Polyphenol-rich blackcurrant extract (BCE) exerts beneficial effects on vascular health in menopausal model animals. However, the vasculoprotective effects in diabetes mellitus (DM) and atherosclerotic vascular disease secondary to DM are unknown. Therefore, we investigated whether BCE is effective in preventing atherosclerosis using KK-A<sup>y</sup> mice as a diabetes model. The mice were divided into three groups and fed a high-fat diet supplemented with 1% BCE (BCE1), 3% BCE (BCE2), or Control for 9 weeks. The mice in the BCE2 group showed a considerable reduction in the disturbance of elastic lamina, foam cell formation, and vascular remodeling compared to those in the BCE1 and Control groups. Immunohistochemical staining indicated that the score of endothelial nitric oxide synthase staining intensity was significantly higher in both BCE2 (2.9) and BCE1 (1.9) compared to that in the Control (1.1). Furthermore, the score for the percentage of alpha-smooth muscle actin was significantly lower in the BCE2 (2.9%) than in the Control (2.1%). Our results suggest that the intake of anthocyanin-rich BCE could have beneficial effects on the blood vessels of diabetic patients.
format	article
author	Kayo Horie Hayato Maeda Naoki Nanashima Indrawati Oey
author_facet	Kayo Horie Hayato Maeda Naoki Nanashima Indrawati Oey
author_sort	Kayo Horie
title	Potential Vasculoprotective Effects of Blackcurrant (<i>Ribes nigrum</i>) Extract in Diabetic KK-A<sup>y</sup> Mice
title_short	Potential Vasculoprotective Effects of Blackcurrant (<i>Ribes nigrum</i>) Extract in Diabetic KK-A<sup>y</sup> Mice
title_full	Potential Vasculoprotective Effects of Blackcurrant (<i>Ribes nigrum</i>) Extract in Diabetic KK-A<sup>y</sup> Mice
title_fullStr	Potential Vasculoprotective Effects of Blackcurrant (<i>Ribes nigrum</i>) Extract in Diabetic KK-A<sup>y</sup> Mice
title_full_unstemmed	Potential Vasculoprotective Effects of Blackcurrant (<i>Ribes nigrum</i>) Extract in Diabetic KK-A<sup>y</sup> Mice
title_sort	potential vasculoprotective effects of blackcurrant (<i>ribes nigrum</i>) extract in diabetic kk-a<sup>y</sup> mice
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/df47a5eafec5490d801833d67dc293dd
work_keys_str_mv	AT kayohorie potentialvasculoprotectiveeffectsofblackcurrantiribesnigrumiextractindiabetickkasupysupmice AT hayatomaeda potentialvasculoprotectiveeffectsofblackcurrantiribesnigrumiextractindiabetickkasupysupmice AT naokinanashima potentialvasculoprotectiveeffectsofblackcurrantiribesnigrumiextractindiabetickkasupysupmice AT indrawatioey potentialvasculoprotectiveeffectsofblackcurrantiribesnigrumiextractindiabetickkasupysupmice
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Potential Vasculoprotective Effects of Blackcurrant (<i>Ribes nigrum</i>) Extract in Diabetic KK-A<sup>y</sup> Mice

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