MicroRNA-29a suppresses the growth of human cervical cancer cells by targeting cell division cycle 42 (CDC42)

Several microRNAs (miRs) play regulatory roles in cervical cancer. The present study investigated the molecular role of microRNA-29a in cervical cancer via modulation of cell division cycle 42 (CDC42) expression. The results showed significant (P < 0.05) downregulation of miR-29a human in cervica...

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Autores principales:	Fang Song, Fengshuang Li
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	cervical cancer micro-rna edu assay mice xenograft tumorigenesis cell cycle arrest Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/df4adb31985e4eda95dfa8425c2d8b9b
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spelling	oai:doaj.org-article:df4adb31985e4eda95dfa8425c2d8b9b2021-11-04T15:00:42ZMicroRNA-29a suppresses the growth of human cervical cancer cells by targeting cell division cycle 42 (CDC42)1658-365510.1080/16583655.2021.1991732https://doaj.org/article/df4adb31985e4eda95dfa8425c2d8b9b2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/16583655.2021.1991732https://doaj.org/toc/1658-3655Several microRNAs (miRs) play regulatory roles in cervical cancer. The present study investigated the molecular role of microRNA-29a in cervical cancer via modulation of cell division cycle 42 (CDC42) expression. The results showed significant (P < 0.05) downregulation of miR-29a human in cervical cancer cells. Overexpression of miR-29a inhibited the proliferation and colony formation of the cervical cancer cells via G0/G1 cell cycle arrest. In silico analysis and luciferase reporter assay showed CDC42 to be the molecular target of miR-29a. CDC42 was found to significantly (P < 0.05) overexpressed in cervical cancer cells. Nonetheless, miR-29 overexpression inhibited the expression of CDC42 in DoTc2 cells. Silencing of CDC42 inhibited the proliferation of DoTc2 cells. However, overexpression of CDC42 could prevent the tumour-suppressive effects of miR-29a. Finally, miR-29a was found to suppress the tumour-growth in vivo further confirming the tumour-suppressive effects of miR-29. Collectively, the findings of the present study revealed the tumour-suppressive effects of miR-29 of cervical cancer.Fang SongFengshuang LiTaylor & Francis Grouparticlecervical cancermicro-rnaedu assaymice xenografttumorigenesiscell cycle arrestScience (General)Q1-390ENJournal of Taibah University for Science, Vol 15, Iss 1, Pp 550-558 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cervical cancer micro-rna edu assay mice xenograft tumorigenesis cell cycle arrest Science (General) Q1-390
spellingShingle	cervical cancer micro-rna edu assay mice xenograft tumorigenesis cell cycle arrest Science (General) Q1-390 Fang Song Fengshuang Li MicroRNA-29a suppresses the growth of human cervical cancer cells by targeting cell division cycle 42 (CDC42)
description	Several microRNAs (miRs) play regulatory roles in cervical cancer. The present study investigated the molecular role of microRNA-29a in cervical cancer via modulation of cell division cycle 42 (CDC42) expression. The results showed significant (P < 0.05) downregulation of miR-29a human in cervical cancer cells. Overexpression of miR-29a inhibited the proliferation and colony formation of the cervical cancer cells via G0/G1 cell cycle arrest. In silico analysis and luciferase reporter assay showed CDC42 to be the molecular target of miR-29a. CDC42 was found to significantly (P < 0.05) overexpressed in cervical cancer cells. Nonetheless, miR-29 overexpression inhibited the expression of CDC42 in DoTc2 cells. Silencing of CDC42 inhibited the proliferation of DoTc2 cells. However, overexpression of CDC42 could prevent the tumour-suppressive effects of miR-29a. Finally, miR-29a was found to suppress the tumour-growth in vivo further confirming the tumour-suppressive effects of miR-29. Collectively, the findings of the present study revealed the tumour-suppressive effects of miR-29 of cervical cancer.
format	article
author	Fang Song Fengshuang Li
author_facet	Fang Song Fengshuang Li
author_sort	Fang Song
title	MicroRNA-29a suppresses the growth of human cervical cancer cells by targeting cell division cycle 42 (CDC42)
title_short	MicroRNA-29a suppresses the growth of human cervical cancer cells by targeting cell division cycle 42 (CDC42)
title_full	MicroRNA-29a suppresses the growth of human cervical cancer cells by targeting cell division cycle 42 (CDC42)
title_fullStr	MicroRNA-29a suppresses the growth of human cervical cancer cells by targeting cell division cycle 42 (CDC42)
title_full_unstemmed	MicroRNA-29a suppresses the growth of human cervical cancer cells by targeting cell division cycle 42 (CDC42)
title_sort	microrna-29a suppresses the growth of human cervical cancer cells by targeting cell division cycle 42 (cdc42)
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/df4adb31985e4eda95dfa8425c2d8b9b
work_keys_str_mv	AT fangsong microrna29asuppressesthegrowthofhumancervicalcancercellsbytargetingcelldivisioncycle42cdc42 AT fengshuangli microrna29asuppressesthegrowthofhumancervicalcancercellsbytargetingcelldivisioncycle42cdc42
_version_	1718444803008495616

MicroRNA-29a suppresses the growth of human cervical cancer cells by targeting cell division cycle 42 (CDC42)

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