Effect of Antibiotics and Gelling Agents in Transformation of Brinjal (Solanum melongena L.) cv. Manjarigota

A study was conducted to find out the effect of antibiotics and gelling agents on Agrobacterium-mediated transformation using hypocotyl explants of brinjal cv. Manjarigota. Hypocotyl explants of brinjal were found to be sensitive even to the lowest level of kanamycin (25 mg/l) tested. Explants that...

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Autores principales:	D P Prakash, B S Deepali, R Asokan, Y L Ramachandra, Lalitha Anand, Vageeshbabu S Hanur
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2007
Materias:	solanum melongena kanamycin cefotaxime gelling agents transformation Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/df4d99e7da904061b105abc79a6a7a73
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spelling	oai:doaj.org-article:df4d99e7da904061b105abc79a6a7a732021-12-02T11:22:28ZEffect of Antibiotics and Gelling Agents in Transformation of Brinjal (Solanum melongena L.) cv. Manjarigota0973-354X2582-4899https://doaj.org/article/df4d99e7da904061b105abc79a6a7a732007-06-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/632https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899A study was conducted to find out the effect of antibiotics and gelling agents on Agrobacterium-mediated transformation using hypocotyl explants of brinjal cv. Manjarigota. Hypocotyl explants of brinjal were found to be sensitive even to the lowest level of kanamycin (25 mg/l) tested. Explants that showed increased callus initiation and regeneration response upon cocultivation with Agrobacterium and on kanamycin at 100 mg/l were selected as this indicated a highly effective selection pressure. Cefotaxime did not affect regeneration response and at 500 mg/l, it effectively inhibited Agrobacterium overgrowth completely on Agrobacterium cocultivated hypocotyl explants. There were marked differences in regeneration response in hypocotyl explants cultured on medium solidified with various gelling agents indicating the influence of gelling agent on the activity of kanamycin in culture medium, which indirectly affects selection and recovery of transformants. Antibiotics and gelling agents could therefore affect, directly or indirectly, transformation of brinjal cv. Manjarigota.D P PrakashB S DeepaliR AsokanY L RamachandraLalitha AnandVageeshbabu S HanurSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticlesolanum melongenakanamycincefotaximegelling agentstransformationPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 2, Iss 1, Pp 19-25 (2007)
institution	DOAJ
collection	DOAJ
language	EN
topic	solanum melongena kanamycin cefotaxime gelling agents transformation Plant culture SB1-1110
spellingShingle	solanum melongena kanamycin cefotaxime gelling agents transformation Plant culture SB1-1110 D P Prakash B S Deepali R Asokan Y L Ramachandra Lalitha Anand Vageeshbabu S Hanur Effect of Antibiotics and Gelling Agents in Transformation of Brinjal (Solanum melongena L.) cv. Manjarigota
description	A study was conducted to find out the effect of antibiotics and gelling agents on Agrobacterium-mediated transformation using hypocotyl explants of brinjal cv. Manjarigota. Hypocotyl explants of brinjal were found to be sensitive even to the lowest level of kanamycin (25 mg/l) tested. Explants that showed increased callus initiation and regeneration response upon cocultivation with Agrobacterium and on kanamycin at 100 mg/l were selected as this indicated a highly effective selection pressure. Cefotaxime did not affect regeneration response and at 500 mg/l, it effectively inhibited Agrobacterium overgrowth completely on Agrobacterium cocultivated hypocotyl explants. There were marked differences in regeneration response in hypocotyl explants cultured on medium solidified with various gelling agents indicating the influence of gelling agent on the activity of kanamycin in culture medium, which indirectly affects selection and recovery of transformants. Antibiotics and gelling agents could therefore affect, directly or indirectly, transformation of brinjal cv. Manjarigota.
format	article
author	D P Prakash B S Deepali R Asokan Y L Ramachandra Lalitha Anand Vageeshbabu S Hanur
author_facet	D P Prakash B S Deepali R Asokan Y L Ramachandra Lalitha Anand Vageeshbabu S Hanur
author_sort	D P Prakash
title	Effect of Antibiotics and Gelling Agents in Transformation of Brinjal (Solanum melongena L.) cv. Manjarigota
title_short	Effect of Antibiotics and Gelling Agents in Transformation of Brinjal (Solanum melongena L.) cv. Manjarigota
title_full	Effect of Antibiotics and Gelling Agents in Transformation of Brinjal (Solanum melongena L.) cv. Manjarigota
title_fullStr	Effect of Antibiotics and Gelling Agents in Transformation of Brinjal (Solanum melongena L.) cv. Manjarigota
title_full_unstemmed	Effect of Antibiotics and Gelling Agents in Transformation of Brinjal (Solanum melongena L.) cv. Manjarigota
title_sort	effect of antibiotics and gelling agents in transformation of brinjal (solanum melongena l.) cv. manjarigota
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2007
url	https://doaj.org/article/df4d99e7da904061b105abc79a6a7a73
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Effect of Antibiotics and Gelling Agents in Transformation of Brinjal (Solanum melongena L.) cv. Manjarigota

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