Digital gene expression analysis of the response to Ralstonia solanacearum between resistant and susceptible tobacco varieties

Abstract Tobacco bacterial wilt (TBW) caused by Ralstonia solanacearum is the most serious soil-borne disease of tobacco. However, molecular mechanism information of R. solanacearum resistance is limited to tobacco, hindering better breeding of resistant tobacco. In this study, the expression profil...

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Autores principales:	YanYan Li, Lin Wang, GuangWei Sun, XiHong Li, ZhenGuo Chen, Ji Feng, Yong Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/df4d9d3a120440e2bf1bc578d561acdb
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spelling	oai:doaj.org-article:df4d9d3a120440e2bf1bc578d561acdb2021-12-02T10:54:31ZDigital gene expression analysis of the response to Ralstonia solanacearum between resistant and susceptible tobacco varieties10.1038/s41598-021-82576-82045-2322https://doaj.org/article/df4d9d3a120440e2bf1bc578d561acdb2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82576-8https://doaj.org/toc/2045-2322Abstract Tobacco bacterial wilt (TBW) caused by Ralstonia solanacearum is the most serious soil-borne disease of tobacco. However, molecular mechanism information of R. solanacearum resistance is limited to tobacco, hindering better breeding of resistant tobacco. In this study, the expression profiles of the rootstalks of Yunyan87 (susceptible cultivar) and Fandi3 (resistant cultivar) at different stages after R. solanacearum infection were compared to explore molecular mechanisms of tobacco resistance against the bacterium. Findings from gene-expression profiling indicated that the number of upregulated differentially expressed genes (DEGs) at 3 and 7 days post-inoculation (dpi) increased significantly in the resistant cultivar. WRKY6 and WRKY11 family genes in WRKY transcription factors, ERF5 and ERF15 family genes in ERFs transcription factors, and genes encoding PR5 were significantly upregulated in the resistant cultivar response to the infection. For the first time, WRKY11 and ERF15 were found to be possibly involved in disease-resistance. The Kyoto Encyclopedia of Genes and Genomes analysis demonstrated glutathione metabolism and phenylpropane pathways as primary resistance pathways to R. solanacearum infection. In the resistant cultivar, DEGs encoding CYP450, TCM, CCoAOMT, 4CL, PAL, CCR, CSE, and CADH, involved in the synthesis of plant antitoxins such as flavonoids, stilbenoids, and lignins, enriched in the phenylpropane pathway were upregulated at 3 and 7 dpi. Furthermore, a pot experiment was performed to verify the role of flavonoids in controlling TBW. This study will strongly contribute to a better understanding of molecular interactions between tobacco plants and R. solanacearum.YanYan LiLin WangGuangWei SunXiHong LiZhenGuo ChenJi FengYong YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q YanYan Li Lin Wang GuangWei Sun XiHong Li ZhenGuo Chen Ji Feng Yong Yang Digital gene expression analysis of the response to Ralstonia solanacearum between resistant and susceptible tobacco varieties
description	Abstract Tobacco bacterial wilt (TBW) caused by Ralstonia solanacearum is the most serious soil-borne disease of tobacco. However, molecular mechanism information of R. solanacearum resistance is limited to tobacco, hindering better breeding of resistant tobacco. In this study, the expression profiles of the rootstalks of Yunyan87 (susceptible cultivar) and Fandi3 (resistant cultivar) at different stages after R. solanacearum infection were compared to explore molecular mechanisms of tobacco resistance against the bacterium. Findings from gene-expression profiling indicated that the number of upregulated differentially expressed genes (DEGs) at 3 and 7 days post-inoculation (dpi) increased significantly in the resistant cultivar. WRKY6 and WRKY11 family genes in WRKY transcription factors, ERF5 and ERF15 family genes in ERFs transcription factors, and genes encoding PR5 were significantly upregulated in the resistant cultivar response to the infection. For the first time, WRKY11 and ERF15 were found to be possibly involved in disease-resistance. The Kyoto Encyclopedia of Genes and Genomes analysis demonstrated glutathione metabolism and phenylpropane pathways as primary resistance pathways to R. solanacearum infection. In the resistant cultivar, DEGs encoding CYP450, TCM, CCoAOMT, 4CL, PAL, CCR, CSE, and CADH, involved in the synthesis of plant antitoxins such as flavonoids, stilbenoids, and lignins, enriched in the phenylpropane pathway were upregulated at 3 and 7 dpi. Furthermore, a pot experiment was performed to verify the role of flavonoids in controlling TBW. This study will strongly contribute to a better understanding of molecular interactions between tobacco plants and R. solanacearum.
format	article
author	YanYan Li Lin Wang GuangWei Sun XiHong Li ZhenGuo Chen Ji Feng Yong Yang
author_facet	YanYan Li Lin Wang GuangWei Sun XiHong Li ZhenGuo Chen Ji Feng Yong Yang
author_sort	YanYan Li
title	Digital gene expression analysis of the response to Ralstonia solanacearum between resistant and susceptible tobacco varieties
title_short	Digital gene expression analysis of the response to Ralstonia solanacearum between resistant and susceptible tobacco varieties
title_full	Digital gene expression analysis of the response to Ralstonia solanacearum between resistant and susceptible tobacco varieties
title_fullStr	Digital gene expression analysis of the response to Ralstonia solanacearum between resistant and susceptible tobacco varieties
title_full_unstemmed	Digital gene expression analysis of the response to Ralstonia solanacearum between resistant and susceptible tobacco varieties
title_sort	digital gene expression analysis of the response to ralstonia solanacearum between resistant and susceptible tobacco varieties
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/df4d9d3a120440e2bf1bc578d561acdb
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Digital gene expression analysis of the response to Ralstonia solanacearum between resistant and susceptible tobacco varieties

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