Evaluation of cellular and humeral immune response of sheep vaccinated with inactivated Rift Valley Fever vaccine adjuvanted with Montanide Gel

Rift Valley Fever (RVF) is an acute infectious zoonotic arthropod - born viral disease, affecting many species of animals with causing great economic losses in animal wealth. Vaccination of susceptible animals with RVF vaccines are an important factor for controlling the disease. This study was appl...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Abd El Rahman S.E., Abul Magd, D. M., Atwa, M. H., Soliman, S. M.
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2020
Materias:	gene expression interleukine (il) real-time polymerase chain reaction (rt-pcr) rift valley fever (rvf) sheep Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2020.21145.1002 https://doaj.org/article/df629f860123491a84adb23edc1bbf8b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:df629f860123491a84adb23edc1bbf8b
record_format	dspace
spelling	oai:doaj.org-article:df629f860123491a84adb23edc1bbf8b2021-12-02T13:21:54ZEvaluation of cellular and humeral immune response of sheep vaccinated with inactivated Rift Valley Fever vaccine adjuvanted with Montanide Gelhttps://dx.doi.org/10.21608/javs.2020.21145.10021687-40722090-3308https://doaj.org/article/df629f860123491a84adb23edc1bbf8b2020-01-01T00:00:00Zhttps://javs.journals.ekb.eg/article_69873.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308Rift Valley Fever (RVF) is an acute infectious zoonotic arthropod - born viral disease, affecting many species of animals with causing great economic losses in animal wealth. Vaccination of susceptible animals with RVF vaccines are an important factor for controlling the disease. This study was applied to improve the locally produced vaccine by using Montanide Gel 01™ as an adjuvant in comparison with Aluminium Hydroxide inactivated RVF vaccine, through evaluating the humeral, cellular immune response and the duration of immunity in sheep vaccinated with the prepared vaccines. The antibody titre in the group vaccinated with one dose of Montanide Gel 01TM inactivated RVF vaccine reached the protective level at the 7th-day post-vaccination and stayed within the protective level till the end of the 11th month, while in the group vaccinated with 2 doses, the antibody titre stayed within the protective level till the end of 13th month in sheep vaccinated with the same vaccine. These results revealed that the best vaccine is 20% Montanide Gel 01TM inactivated RVF vaccine (2 doses) as it gave a higher level of antibody throughout the experiment compared with that of other vaccinated groups. Also, the results of cell-mediated immune response showed that: (i) The cell proliferation expressed by optical density showed early significance in sheep vaccinated by Montanide Gel 01TM inactivated RVF vaccine and a slight elevation in sheep vaccinated by Aluminium Hydroxide inactivated RVF vaccine. (ii) The phagocytic activity expressed by phagocytic percentage and phagocytic index showed early significant in sheep vaccinated with Montanide Gel 01TM inactivated RVF vaccine and a slight elevation in sheep vaccinated by Aluminium hydroxide inactivated RVF vaccine. Also, high levels of IL-6 and IL-12 gene expression were detected in sheep vaccinated with Montanide Gel 01TM inactivated RVF vaccine as shown by RT-PCR. All the previous data showed that the Montanide Gel 01TM was highly immunogenic than Aluminium Hydroxide beside that Montanide Gel 01TM inactivated RVF vaccine induces rapid onset immunological response with a long duration which recommended for emergency vaccination. Abd El Rahman S.E.Abul Magd, D. M.Atwa, M. H.Soliman, S. M.Egyptian Society for Animal Managementarticlegene expressioninterleukine (il)real-time polymerase chain reaction (rt-pcr)rift valley fever (rvf)sheepZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 5, Iss 1, Pp 22-34 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	gene expression interleukine (il) real-time polymerase chain reaction (rt-pcr) rift valley fever (rvf) sheep Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	gene expression interleukine (il) real-time polymerase chain reaction (rt-pcr) rift valley fever (rvf) sheep Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Abd El Rahman S.E. Abul Magd, D. M. Atwa, M. H. Soliman, S. M. Evaluation of cellular and humeral immune response of sheep vaccinated with inactivated Rift Valley Fever vaccine adjuvanted with Montanide Gel
description	Rift Valley Fever (RVF) is an acute infectious zoonotic arthropod - born viral disease, affecting many species of animals with causing great economic losses in animal wealth. Vaccination of susceptible animals with RVF vaccines are an important factor for controlling the disease. This study was applied to improve the locally produced vaccine by using Montanide Gel 01™ as an adjuvant in comparison with Aluminium Hydroxide inactivated RVF vaccine, through evaluating the humeral, cellular immune response and the duration of immunity in sheep vaccinated with the prepared vaccines. The antibody titre in the group vaccinated with one dose of Montanide Gel 01TM inactivated RVF vaccine reached the protective level at the 7th-day post-vaccination and stayed within the protective level till the end of the 11th month, while in the group vaccinated with 2 doses, the antibody titre stayed within the protective level till the end of 13th month in sheep vaccinated with the same vaccine. These results revealed that the best vaccine is 20% Montanide Gel 01TM inactivated RVF vaccine (2 doses) as it gave a higher level of antibody throughout the experiment compared with that of other vaccinated groups. Also, the results of cell-mediated immune response showed that: (i) The cell proliferation expressed by optical density showed early significance in sheep vaccinated by Montanide Gel 01TM inactivated RVF vaccine and a slight elevation in sheep vaccinated by Aluminium Hydroxide inactivated RVF vaccine. (ii) The phagocytic activity expressed by phagocytic percentage and phagocytic index showed early significant in sheep vaccinated with Montanide Gel 01TM inactivated RVF vaccine and a slight elevation in sheep vaccinated by Aluminium hydroxide inactivated RVF vaccine. Also, high levels of IL-6 and IL-12 gene expression were detected in sheep vaccinated with Montanide Gel 01TM inactivated RVF vaccine as shown by RT-PCR. All the previous data showed that the Montanide Gel 01TM was highly immunogenic than Aluminium Hydroxide beside that Montanide Gel 01TM inactivated RVF vaccine induces rapid onset immunological response with a long duration which recommended for emergency vaccination.
format	article
author	Abd El Rahman S.E. Abul Magd, D. M. Atwa, M. H. Soliman, S. M.
author_facet	Abd El Rahman S.E. Abul Magd, D. M. Atwa, M. H. Soliman, S. M.
author_sort	Abd El Rahman S.E.
title	Evaluation of cellular and humeral immune response of sheep vaccinated with inactivated Rift Valley Fever vaccine adjuvanted with Montanide Gel
title_short	Evaluation of cellular and humeral immune response of sheep vaccinated with inactivated Rift Valley Fever vaccine adjuvanted with Montanide Gel
title_full	Evaluation of cellular and humeral immune response of sheep vaccinated with inactivated Rift Valley Fever vaccine adjuvanted with Montanide Gel
title_fullStr	Evaluation of cellular and humeral immune response of sheep vaccinated with inactivated Rift Valley Fever vaccine adjuvanted with Montanide Gel
title_full_unstemmed	Evaluation of cellular and humeral immune response of sheep vaccinated with inactivated Rift Valley Fever vaccine adjuvanted with Montanide Gel
title_sort	evaluation of cellular and humeral immune response of sheep vaccinated with inactivated rift valley fever vaccine adjuvanted with montanide gel
publisher	Egyptian Society for Animal Management
publishDate	2020
url	https://dx.doi.org/10.21608/javs.2020.21145.1002 https://doaj.org/article/df629f860123491a84adb23edc1bbf8b
work_keys_str_mv	AT abdelrahmanse evaluationofcellularandhumeralimmuneresponseofsheepvaccinatedwithinactivatedriftvalleyfevervaccineadjuvantedwithmontanidegel AT abulmagddm evaluationofcellularandhumeralimmuneresponseofsheepvaccinatedwithinactivatedriftvalleyfevervaccineadjuvantedwithmontanidegel AT atwamh evaluationofcellularandhumeralimmuneresponseofsheepvaccinatedwithinactivatedriftvalleyfevervaccineadjuvantedwithmontanidegel AT solimansm evaluationofcellularandhumeralimmuneresponseofsheepvaccinatedwithinactivatedriftvalleyfevervaccineadjuvantedwithmontanidegel
_version_	1718393184325730304

Evaluation of cellular and humeral immune response of sheep vaccinated with inactivated Rift Valley Fever vaccine adjuvanted with Montanide Gel

Ejemplares similares