Integrated Study of Transcriptome-wide m6A Methylome Reveals Novel Insights Into the Character and Function of m6A Methylation During Yak Adipocyte Differentiation

Yak (Bos grunniens) is considered an iconic symbol of Tibet and high altitude, but they suffer from malnutrition during the cold season that challenges the metabolism of energy. Adipocytes perform a crucial role in maintaining the energy balance, and adipocyte differentiation is a complex process in...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yongfeng Zhang, Chunnian Liang, Xiaoyun Wu, Jie Pei, Xian Guo, Min Chu, Xuezhi Ding, Pengjia Bao, Qudratullah Kalwar, Ping Yan
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	yak adipocyte N6-methyladenosine MeRIP-seq regulatory mechanism Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/df62c4b46eb04e348930ecdcb9cd59f6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:df62c4b46eb04e348930ecdcb9cd59f6
record_format	dspace
spelling	oai:doaj.org-article:df62c4b46eb04e348930ecdcb9cd59f62021-12-03T07:09:47ZIntegrated Study of Transcriptome-wide m6A Methylome Reveals Novel Insights Into the Character and Function of m6A Methylation During Yak Adipocyte Differentiation2296-634X10.3389/fcell.2021.689067https://doaj.org/article/df62c4b46eb04e348930ecdcb9cd59f62021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.689067/fullhttps://doaj.org/toc/2296-634XYak (Bos grunniens) is considered an iconic symbol of Tibet and high altitude, but they suffer from malnutrition during the cold season that challenges the metabolism of energy. Adipocytes perform a crucial role in maintaining the energy balance, and adipocyte differentiation is a complex process involving multiple changes in the expression of genes. N6-methyladenosine (m6A) plays a dynamic role in post-transcription gene expression regulation as the most widespread mRNA modification of the higher eukaryotes. However, currently there is no research existing on the m6A transcriptome-wide map of bovine animals and their potential biological functions in adipocyte differentiation. Therefore, we performed methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) to determine the distinctions in m6A methylation and gene expression during yak adipocyte differentiation. In yak adipocyte and preadipocyte the content of m6A and m6A-associated enzymes was substantially different. In the two groups, a total of 14,710 m6A peaks and 13,388 m6A peaks were identified. For the most part, m6A peaks were enriched in stop codons, 3′-untranslated regions, and coding regions with consensus motifs of GGACU. The functional enrichment exploration displayed that differentially methylated genes participated in some of the pathways associated with adipogenic metabolism, and several candidate genes (KLF9, FOXO1, ZNF395, and UHRF1) were involved in these pathways. In addition to that, there was a positive association between m6A abundance and levels of gene expression, which displayed that m6A may play a vital role in modulating gene expression during yak adipocyte differentiation. Further, in the adipocyte group, several methylation gene protein expression levels were significantly higher than in preadipocytes. In short, it can be concluded that the current study provides a comprehensive explanation of the m6A features in the yak transcriptome, offering in-depth insights into m6A topology and associated molecular mechanisms underlying bovine adipocyte differentiation, which might be helpful for further understanding its mechanisms.Yongfeng ZhangYongfeng ZhangChunnian LiangXiaoyun WuJie PeiXian GuoMin ChuXuezhi DingPengjia BaoQudratullah KalwarPing YanPing YanFrontiers Media S.A.articleyakadipocyteN6-methyladenosineMeRIP-seqregulatory mechanismBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	yak adipocyte N6-methyladenosine MeRIP-seq regulatory mechanism Biology (General) QH301-705.5
spellingShingle	yak adipocyte N6-methyladenosine MeRIP-seq regulatory mechanism Biology (General) QH301-705.5 Yongfeng Zhang Yongfeng Zhang Chunnian Liang Xiaoyun Wu Jie Pei Xian Guo Min Chu Xuezhi Ding Pengjia Bao Qudratullah Kalwar Ping Yan Ping Yan Integrated Study of Transcriptome-wide m6A Methylome Reveals Novel Insights Into the Character and Function of m6A Methylation During Yak Adipocyte Differentiation
description	Yak (Bos grunniens) is considered an iconic symbol of Tibet and high altitude, but they suffer from malnutrition during the cold season that challenges the metabolism of energy. Adipocytes perform a crucial role in maintaining the energy balance, and adipocyte differentiation is a complex process involving multiple changes in the expression of genes. N6-methyladenosine (m6A) plays a dynamic role in post-transcription gene expression regulation as the most widespread mRNA modification of the higher eukaryotes. However, currently there is no research existing on the m6A transcriptome-wide map of bovine animals and their potential biological functions in adipocyte differentiation. Therefore, we performed methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) to determine the distinctions in m6A methylation and gene expression during yak adipocyte differentiation. In yak adipocyte and preadipocyte the content of m6A and m6A-associated enzymes was substantially different. In the two groups, a total of 14,710 m6A peaks and 13,388 m6A peaks were identified. For the most part, m6A peaks were enriched in stop codons, 3′-untranslated regions, and coding regions with consensus motifs of GGACU. The functional enrichment exploration displayed that differentially methylated genes participated in some of the pathways associated with adipogenic metabolism, and several candidate genes (KLF9, FOXO1, ZNF395, and UHRF1) were involved in these pathways. In addition to that, there was a positive association between m6A abundance and levels of gene expression, which displayed that m6A may play a vital role in modulating gene expression during yak adipocyte differentiation. Further, in the adipocyte group, several methylation gene protein expression levels were significantly higher than in preadipocytes. In short, it can be concluded that the current study provides a comprehensive explanation of the m6A features in the yak transcriptome, offering in-depth insights into m6A topology and associated molecular mechanisms underlying bovine adipocyte differentiation, which might be helpful for further understanding its mechanisms.
format	article
author	Yongfeng Zhang Yongfeng Zhang Chunnian Liang Xiaoyun Wu Jie Pei Xian Guo Min Chu Xuezhi Ding Pengjia Bao Qudratullah Kalwar Ping Yan Ping Yan
author_facet	Yongfeng Zhang Yongfeng Zhang Chunnian Liang Xiaoyun Wu Jie Pei Xian Guo Min Chu Xuezhi Ding Pengjia Bao Qudratullah Kalwar Ping Yan Ping Yan
author_sort	Yongfeng Zhang
title	Integrated Study of Transcriptome-wide m6A Methylome Reveals Novel Insights Into the Character and Function of m6A Methylation During Yak Adipocyte Differentiation
title_short	Integrated Study of Transcriptome-wide m6A Methylome Reveals Novel Insights Into the Character and Function of m6A Methylation During Yak Adipocyte Differentiation
title_full	Integrated Study of Transcriptome-wide m6A Methylome Reveals Novel Insights Into the Character and Function of m6A Methylation During Yak Adipocyte Differentiation
title_fullStr	Integrated Study of Transcriptome-wide m6A Methylome Reveals Novel Insights Into the Character and Function of m6A Methylation During Yak Adipocyte Differentiation
title_full_unstemmed	Integrated Study of Transcriptome-wide m6A Methylome Reveals Novel Insights Into the Character and Function of m6A Methylation During Yak Adipocyte Differentiation
title_sort	integrated study of transcriptome-wide m6a methylome reveals novel insights into the character and function of m6a methylation during yak adipocyte differentiation
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/df62c4b46eb04e348930ecdcb9cd59f6
work_keys_str_mv	AT yongfengzhang integratedstudyoftranscriptomewidem6amethylomerevealsnovelinsightsintothecharacterandfunctionofm6amethylationduringyakadipocytedifferentiation AT yongfengzhang integratedstudyoftranscriptomewidem6amethylomerevealsnovelinsightsintothecharacterandfunctionofm6amethylationduringyakadipocytedifferentiation AT chunnianliang integratedstudyoftranscriptomewidem6amethylomerevealsnovelinsightsintothecharacterandfunctionofm6amethylationduringyakadipocytedifferentiation AT xiaoyunwu integratedstudyoftranscriptomewidem6amethylomerevealsnovelinsightsintothecharacterandfunctionofm6amethylationduringyakadipocytedifferentiation AT jiepei integratedstudyoftranscriptomewidem6amethylomerevealsnovelinsightsintothecharacterandfunctionofm6amethylationduringyakadipocytedifferentiation AT xianguo integratedstudyoftranscriptomewidem6amethylomerevealsnovelinsightsintothecharacterandfunctionofm6amethylationduringyakadipocytedifferentiation AT minchu integratedstudyoftranscriptomewidem6amethylomerevealsnovelinsightsintothecharacterandfunctionofm6amethylationduringyakadipocytedifferentiation AT xuezhiding integratedstudyoftranscriptomewidem6amethylomerevealsnovelinsightsintothecharacterandfunctionofm6amethylationduringyakadipocytedifferentiation AT pengjiabao integratedstudyoftranscriptomewidem6amethylomerevealsnovelinsightsintothecharacterandfunctionofm6amethylationduringyakadipocytedifferentiation AT qudratullahkalwar integratedstudyoftranscriptomewidem6amethylomerevealsnovelinsightsintothecharacterandfunctionofm6amethylationduringyakadipocytedifferentiation AT pingyan integratedstudyoftranscriptomewidem6amethylomerevealsnovelinsightsintothecharacterandfunctionofm6amethylationduringyakadipocytedifferentiation AT pingyan integratedstudyoftranscriptomewidem6amethylomerevealsnovelinsightsintothecharacterandfunctionofm6amethylationduringyakadipocytedifferentiation
_version_	1718373903330443264

Integrated Study of Transcriptome-wide m6A Methylome Reveals Novel Insights Into the Character and Function of m6A Methylation During Yak Adipocyte Differentiation

Ejemplares similares