Anti-cancerous effect of albumin coated silver nanoparticles on MDA-MB 231 human breast cancer cell line

Abstract With the aim of making specific targeting of silver nanoparticles as a drug for tumor cells and developing new anticancer agents, a novel nano-composite was developed. Albumin coated silver nanoparticles (ASNPs) were synthesized, and their anti-cancerous effects were evaluated against MDA-M...

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Autores principales:	Marzieh Azizi, Hedayatoallah Ghourchian, Fatemeh Yazdian, Shahla Bagherifam, Sara Bekhradnia, Bo Nyström
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/df6689945ecb4bd99b9dd3932e356c6b
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spelling	oai:doaj.org-article:df6689945ecb4bd99b9dd3932e356c6b2021-12-02T11:52:23ZAnti-cancerous effect of albumin coated silver nanoparticles on MDA-MB 231 human breast cancer cell line10.1038/s41598-017-05461-32045-2322https://doaj.org/article/df6689945ecb4bd99b9dd3932e356c6b2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05461-3https://doaj.org/toc/2045-2322Abstract With the aim of making specific targeting of silver nanoparticles as a drug for tumor cells and developing new anticancer agents, a novel nano-composite was developed. Albumin coated silver nanoparticles (ASNPs) were synthesized, and their anti-cancerous effects were evaluated against MDA-MB 231, a human breast cancer cell line. The synthesized ASNPs were characterized by spectroscopic methods. The morphological changes of the cells were observed by inverted, florescent microscopy and also by DNA ladder pattern on gel electrophoresis; the results revealed that the cell death process occurred through the apoptosis mechanism. It was found that ASNPs with a size of 90 nm and negatively charged with a zeta-potential of about −20 mV could be specifically taken up by tumor cells. The LD50 of ASNPs against MDA-MB 231 (5 μM), was found to be 30 times higher than that for white normal blood cells (152 μM). The characteristics of the synthesized ASNPs included; intact structure of coated albumin, higher cytotoxicity against cancer cells than over normal cells, and cell death based on apoptosis and reduction of gland tumor sizes in mice. This work indicates that ASNPs could be a good candidate for chemotherapeutic drug.Marzieh AziziHedayatoallah GhourchianFatemeh YazdianShahla BagherifamSara BekhradniaBo NyströmNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-18 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marzieh Azizi Hedayatoallah Ghourchian Fatemeh Yazdian Shahla Bagherifam Sara Bekhradnia Bo Nyström Anti-cancerous effect of albumin coated silver nanoparticles on MDA-MB 231 human breast cancer cell line
description	Abstract With the aim of making specific targeting of silver nanoparticles as a drug for tumor cells and developing new anticancer agents, a novel nano-composite was developed. Albumin coated silver nanoparticles (ASNPs) were synthesized, and their anti-cancerous effects were evaluated against MDA-MB 231, a human breast cancer cell line. The synthesized ASNPs were characterized by spectroscopic methods. The morphological changes of the cells were observed by inverted, florescent microscopy and also by DNA ladder pattern on gel electrophoresis; the results revealed that the cell death process occurred through the apoptosis mechanism. It was found that ASNPs with a size of 90 nm and negatively charged with a zeta-potential of about −20 mV could be specifically taken up by tumor cells. The LD50 of ASNPs against MDA-MB 231 (5 μM), was found to be 30 times higher than that for white normal blood cells (152 μM). The characteristics of the synthesized ASNPs included; intact structure of coated albumin, higher cytotoxicity against cancer cells than over normal cells, and cell death based on apoptosis and reduction of gland tumor sizes in mice. This work indicates that ASNPs could be a good candidate for chemotherapeutic drug.
format	article
author	Marzieh Azizi Hedayatoallah Ghourchian Fatemeh Yazdian Shahla Bagherifam Sara Bekhradnia Bo Nyström
author_facet	Marzieh Azizi Hedayatoallah Ghourchian Fatemeh Yazdian Shahla Bagherifam Sara Bekhradnia Bo Nyström
author_sort	Marzieh Azizi
title	Anti-cancerous effect of albumin coated silver nanoparticles on MDA-MB 231 human breast cancer cell line
title_short	Anti-cancerous effect of albumin coated silver nanoparticles on MDA-MB 231 human breast cancer cell line
title_full	Anti-cancerous effect of albumin coated silver nanoparticles on MDA-MB 231 human breast cancer cell line
title_fullStr	Anti-cancerous effect of albumin coated silver nanoparticles on MDA-MB 231 human breast cancer cell line
title_full_unstemmed	Anti-cancerous effect of albumin coated silver nanoparticles on MDA-MB 231 human breast cancer cell line
title_sort	anti-cancerous effect of albumin coated silver nanoparticles on mda-mb 231 human breast cancer cell line
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/df6689945ecb4bd99b9dd3932e356c6b
work_keys_str_mv	AT marziehazizi anticancerouseffectofalbumincoatedsilvernanoparticlesonmdamb231humanbreastcancercellline AT hedayatoallahghourchian anticancerouseffectofalbumincoatedsilvernanoparticlesonmdamb231humanbreastcancercellline AT fatemehyazdian anticancerouseffectofalbumincoatedsilvernanoparticlesonmdamb231humanbreastcancercellline AT shahlabagherifam anticancerouseffectofalbumincoatedsilvernanoparticlesonmdamb231humanbreastcancercellline AT sarabekhradnia anticancerouseffectofalbumincoatedsilvernanoparticlesonmdamb231humanbreastcancercellline AT bonystrom anticancerouseffectofalbumincoatedsilvernanoparticlesonmdamb231humanbreastcancercellline
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Anti-cancerous effect of albumin coated silver nanoparticles on MDA-MB 231 human breast cancer cell line

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