Treatment with atrial natriuretic peptide induces adipose tissue browning and exerts thermogenic actions in vivo

Abstract Increasing evidence suggests natriuretic peptides (NPs) coordinate inter-organ metabolic crosstalk with adipose tissues and play a critical role in energy metabolism. We recently reported A-type NP (ANP) raises intracellular temperature in cultured adipocytes in a low-temperature-sensitive...

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Autores principales:	Haruka Kimura, Tomohisa Nagoshi, Yuhei Oi, Akira Yoshii, Yoshiro Tanaka, Hirotake Takahashi, Yusuke Kashiwagi, Toshikazu D. Tanaka, Michihiro Yoshimura
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/df6b1060b76d4735a8324161aae50b39
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spelling	oai:doaj.org-article:df6b1060b76d4735a8324161aae50b392021-12-02T19:04:01ZTreatment with atrial natriuretic peptide induces adipose tissue browning and exerts thermogenic actions in vivo10.1038/s41598-021-96970-92045-2322https://doaj.org/article/df6b1060b76d4735a8324161aae50b392021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96970-9https://doaj.org/toc/2045-2322Abstract Increasing evidence suggests natriuretic peptides (NPs) coordinate inter-organ metabolic crosstalk with adipose tissues and play a critical role in energy metabolism. We recently reported A-type NP (ANP) raises intracellular temperature in cultured adipocytes in a low-temperature-sensitive manner. We herein investigated whether exogenous ANP-treatment exerts a significant impact on adipose tissues in vivo. Mice fed a high-fat-diet (HFD) or normal-fat-diet (NFD) for 13 weeks were treated with or without ANP infusion subcutaneously for another 3 weeks. ANP-treatment significantly ameliorated HFD-induced insulin resistance. HFD increased brown adipose tissue (BAT) cell size with the accumulation of lipid droplets (whitening), which was suppressed by ANP-treatment (re-browning). Furthermore, HFD induced enlarged lipid droplets in inguinal white adipose tissue (iWAT), crown-like structures in epididymal WAT, and hepatic steatosis, all of which were substantially attenuated by ANP-treatment. Likewise, ANP-treatment markedly increased UCP1 expression, a specific marker of BAT, in iWAT (browning). ANP also further increased UCP1 expression in BAT with NFD. Accordingly, cold tolerance test demonstrated ANP-treated mice were tolerant to cold exposure. In summary, exogenous ANP administration ameliorates HFD-induced insulin resistance by attenuating hepatic steatosis and by inducing adipose tissue browning (activation of the adipose tissue thermogenic program), leading to in vivo thermogenesis during cold exposure.Haruka KimuraTomohisa NagoshiYuhei OiAkira YoshiiYoshiro TanakaHirotake TakahashiYusuke KashiwagiToshikazu D. TanakaMichihiro YoshimuraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Haruka Kimura Tomohisa Nagoshi Yuhei Oi Akira Yoshii Yoshiro Tanaka Hirotake Takahashi Yusuke Kashiwagi Toshikazu D. Tanaka Michihiro Yoshimura Treatment with atrial natriuretic peptide induces adipose tissue browning and exerts thermogenic actions in vivo
description	Abstract Increasing evidence suggests natriuretic peptides (NPs) coordinate inter-organ metabolic crosstalk with adipose tissues and play a critical role in energy metabolism. We recently reported A-type NP (ANP) raises intracellular temperature in cultured adipocytes in a low-temperature-sensitive manner. We herein investigated whether exogenous ANP-treatment exerts a significant impact on adipose tissues in vivo. Mice fed a high-fat-diet (HFD) or normal-fat-diet (NFD) for 13 weeks were treated with or without ANP infusion subcutaneously for another 3 weeks. ANP-treatment significantly ameliorated HFD-induced insulin resistance. HFD increased brown adipose tissue (BAT) cell size with the accumulation of lipid droplets (whitening), which was suppressed by ANP-treatment (re-browning). Furthermore, HFD induced enlarged lipid droplets in inguinal white adipose tissue (iWAT), crown-like structures in epididymal WAT, and hepatic steatosis, all of which were substantially attenuated by ANP-treatment. Likewise, ANP-treatment markedly increased UCP1 expression, a specific marker of BAT, in iWAT (browning). ANP also further increased UCP1 expression in BAT with NFD. Accordingly, cold tolerance test demonstrated ANP-treated mice were tolerant to cold exposure. In summary, exogenous ANP administration ameliorates HFD-induced insulin resistance by attenuating hepatic steatosis and by inducing adipose tissue browning (activation of the adipose tissue thermogenic program), leading to in vivo thermogenesis during cold exposure.
format	article
author	Haruka Kimura Tomohisa Nagoshi Yuhei Oi Akira Yoshii Yoshiro Tanaka Hirotake Takahashi Yusuke Kashiwagi Toshikazu D. Tanaka Michihiro Yoshimura
author_facet	Haruka Kimura Tomohisa Nagoshi Yuhei Oi Akira Yoshii Yoshiro Tanaka Hirotake Takahashi Yusuke Kashiwagi Toshikazu D. Tanaka Michihiro Yoshimura
author_sort	Haruka Kimura
title	Treatment with atrial natriuretic peptide induces adipose tissue browning and exerts thermogenic actions in vivo
title_short	Treatment with atrial natriuretic peptide induces adipose tissue browning and exerts thermogenic actions in vivo
title_full	Treatment with atrial natriuretic peptide induces adipose tissue browning and exerts thermogenic actions in vivo
title_fullStr	Treatment with atrial natriuretic peptide induces adipose tissue browning and exerts thermogenic actions in vivo
title_full_unstemmed	Treatment with atrial natriuretic peptide induces adipose tissue browning and exerts thermogenic actions in vivo
title_sort	treatment with atrial natriuretic peptide induces adipose tissue browning and exerts thermogenic actions in vivo
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/df6b1060b76d4735a8324161aae50b39
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Treatment with atrial natriuretic peptide induces adipose tissue browning and exerts thermogenic actions in vivo

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