Self-assembled Collagen-Fibrin Hydrogel Reinforces Tissue Engineered Adventitia Vessels Seeded with Human Fibroblasts

Abstract Efforts for tissue engineering vascular grafts focuses on the tunica media and intima, although the tunica adventitia serves as the primary structural support for blood vessels. In surgery, during endarterectomies, surgeons can strip the vessel, leaving the adventitia as the main strength l...

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Autores principales:	Bijal Patel, Zhengfan Xu, Cameron B. Pinnock, Loay S. Kabbani, Mai T. Lam
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/df6d459d4cd14e69a5c6d0cb5783874c
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spelling	oai:doaj.org-article:df6d459d4cd14e69a5c6d0cb5783874c2021-12-02T15:05:02ZSelf-assembled Collagen-Fibrin Hydrogel Reinforces Tissue Engineered Adventitia Vessels Seeded with Human Fibroblasts10.1038/s41598-018-21681-72045-2322https://doaj.org/article/df6d459d4cd14e69a5c6d0cb5783874c2018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21681-7https://doaj.org/toc/2045-2322Abstract Efforts for tissue engineering vascular grafts focuses on the tunica media and intima, although the tunica adventitia serves as the primary structural support for blood vessels. In surgery, during endarterectomies, surgeons can strip the vessel, leaving the adventitia as the main strength layer to close the vessel. Here, we adapted our recently developed technique of forming vascular tissue rings then stacking the rings into a tubular structure, to accommodate human fibroblasts to create adventitia vessels in 8 days. Collagen production and fibril cross-linking was augmented with TGF-β and ascorbic acid, significantly increasing tensile strength to 57.8 ± 3.07 kPa (p = 0.008). Collagen type I gel was added to the base fibrin hydrogel to further increase strength. Groups were: Fibrin only; 0.7 mg/ml COL; 1.7 mg/ml COL; and 2.2 mg/ml COL. The 0.7 mg/ml collagen rings resulted in the highest tensile strength at 77.0 ± 18.1 kPa (p = 0.015). Culture periods of 1–2 weeks resulted in an increase in extracellular matrix deposition and significantly higher failure strength but not ultimate tensile strength. Histological analysis showed the 0.7 mg/ml COL group had significantly more, mature collagen. Thus, a hydrogel of 0.7 mg/ml collagen in fibrin was ideal for creating and strengthening engineered adventitia vessels.Bijal PatelZhengfan XuCameron B. PinnockLoay S. KabbaniMai T. LamNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bijal Patel Zhengfan Xu Cameron B. Pinnock Loay S. Kabbani Mai T. Lam Self-assembled Collagen-Fibrin Hydrogel Reinforces Tissue Engineered Adventitia Vessels Seeded with Human Fibroblasts
description	Abstract Efforts for tissue engineering vascular grafts focuses on the tunica media and intima, although the tunica adventitia serves as the primary structural support for blood vessels. In surgery, during endarterectomies, surgeons can strip the vessel, leaving the adventitia as the main strength layer to close the vessel. Here, we adapted our recently developed technique of forming vascular tissue rings then stacking the rings into a tubular structure, to accommodate human fibroblasts to create adventitia vessels in 8 days. Collagen production and fibril cross-linking was augmented with TGF-β and ascorbic acid, significantly increasing tensile strength to 57.8 ± 3.07 kPa (p = 0.008). Collagen type I gel was added to the base fibrin hydrogel to further increase strength. Groups were: Fibrin only; 0.7 mg/ml COL; 1.7 mg/ml COL; and 2.2 mg/ml COL. The 0.7 mg/ml collagen rings resulted in the highest tensile strength at 77.0 ± 18.1 kPa (p = 0.015). Culture periods of 1–2 weeks resulted in an increase in extracellular matrix deposition and significantly higher failure strength but not ultimate tensile strength. Histological analysis showed the 0.7 mg/ml COL group had significantly more, mature collagen. Thus, a hydrogel of 0.7 mg/ml collagen in fibrin was ideal for creating and strengthening engineered adventitia vessels.
format	article
author	Bijal Patel Zhengfan Xu Cameron B. Pinnock Loay S. Kabbani Mai T. Lam
author_facet	Bijal Patel Zhengfan Xu Cameron B. Pinnock Loay S. Kabbani Mai T. Lam
author_sort	Bijal Patel
title	Self-assembled Collagen-Fibrin Hydrogel Reinforces Tissue Engineered Adventitia Vessels Seeded with Human Fibroblasts
title_short	Self-assembled Collagen-Fibrin Hydrogel Reinforces Tissue Engineered Adventitia Vessels Seeded with Human Fibroblasts
title_full	Self-assembled Collagen-Fibrin Hydrogel Reinforces Tissue Engineered Adventitia Vessels Seeded with Human Fibroblasts
title_fullStr	Self-assembled Collagen-Fibrin Hydrogel Reinforces Tissue Engineered Adventitia Vessels Seeded with Human Fibroblasts
title_full_unstemmed	Self-assembled Collagen-Fibrin Hydrogel Reinforces Tissue Engineered Adventitia Vessels Seeded with Human Fibroblasts
title_sort	self-assembled collagen-fibrin hydrogel reinforces tissue engineered adventitia vessels seeded with human fibroblasts
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/df6d459d4cd14e69a5c6d0cb5783874c
work_keys_str_mv	AT bijalpatel selfassembledcollagenfibrinhydrogelreinforcestissueengineeredadventitiavesselsseededwithhumanfibroblasts AT zhengfanxu selfassembledcollagenfibrinhydrogelreinforcestissueengineeredadventitiavesselsseededwithhumanfibroblasts AT cameronbpinnock selfassembledcollagenfibrinhydrogelreinforcestissueengineeredadventitiavesselsseededwithhumanfibroblasts AT loayskabbani selfassembledcollagenfibrinhydrogelreinforcestissueengineeredadventitiavesselsseededwithhumanfibroblasts AT maitlam selfassembledcollagenfibrinhydrogelreinforcestissueengineeredadventitiavesselsseededwithhumanfibroblasts
_version_	1718388987943452672

Self-assembled Collagen-Fibrin Hydrogel Reinforces Tissue Engineered Adventitia Vessels Seeded with Human Fibroblasts

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