Transfer of Auxinic Herbicide Resistance from Wild Mustard (Sinapis arvensis) into Radish (Raphanus sativus) through Embryo Rescue

The discovery of auxinic herbicides (e.g., 2,4-D, Dicamba, Picloram) for selective control of broad-leaf weeds in cereal crops revolutionized modern agriculture. These herbicides are inexpensive and do not generally have prolonged residual activity in soil. Although cultivated species of Brassicacea...

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Autores principales:	J Mithila, J Christopher Hall
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2012
Materias:	auxinic herbicide embryo rescue radish resistance transfer wild mustard Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/df732f144f4d42d4a913b851173a176a
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spelling	oai:doaj.org-article:df732f144f4d42d4a913b851173a176a2021-12-02T04:02:09ZTransfer of Auxinic Herbicide Resistance from Wild Mustard (Sinapis arvensis) into Radish (Raphanus sativus) through Embryo Rescue0973-354X2582-4899https://doaj.org/article/df732f144f4d42d4a913b851173a176a2012-06-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/384https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899The discovery of auxinic herbicides (e.g., 2,4-D, Dicamba, Picloram) for selective control of broad-leaf weeds in cereal crops revolutionized modern agriculture. These herbicides are inexpensive and do not generally have prolonged residual activity in soil. Although cultivated species of Brassicaceae (e.g., radish and other vegetables) are susceptible to auxinic herbicides, some biotypes of wild mustard (Sinapis arvensis, 2n = 18) were found to be highly resistant to Picloram and Dicamba. Inter-generic hybrids between wild mustard and radish (Raphanus sativus, 2n = 18) were produced by traditional breeding coupled with in vitro embryo rescue/ovule culture. To increase frequency of embryo regeneration and hybrid plant production, several hundred reciprocal crosses were performed between these species. Upon altering cultural conditions and media composition, a high frequency of embryo regeneration and hybrid plant establishment was achieved. A protocol was also optimized for in vitro clonal multiplication of inter-generic hybrids produced by embryo rescue. To evaluate transfer of auxinic herbicide resistance from wild mustard into hybrid plants, several screening tests (involving in vitro, molecular-based as well as whole plant-based tests) were performed. Results indicated that hybrids of R. sativus x S. arevensis were resistant to auxinic herbicides suggesting, that, the resistance trait was transferred to these hybrids from the wild mustard. This research for the first time demonstrates the possibility of transfer of auxinic herbicide resistance from wild mustard to radish.J MithilaJ Christopher HallSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticleauxinic herbicideembryo rescueradishresistancetransferwild mustardPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 7, Iss 1, Pp 29-33 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	auxinic herbicide embryo rescue radish resistance transfer wild mustard Plant culture SB1-1110
spellingShingle	auxinic herbicide embryo rescue radish resistance transfer wild mustard Plant culture SB1-1110 J Mithila J Christopher Hall Transfer of Auxinic Herbicide Resistance from Wild Mustard (Sinapis arvensis) into Radish (Raphanus sativus) through Embryo Rescue
description	The discovery of auxinic herbicides (e.g., 2,4-D, Dicamba, Picloram) for selective control of broad-leaf weeds in cereal crops revolutionized modern agriculture. These herbicides are inexpensive and do not generally have prolonged residual activity in soil. Although cultivated species of Brassicaceae (e.g., radish and other vegetables) are susceptible to auxinic herbicides, some biotypes of wild mustard (Sinapis arvensis, 2n = 18) were found to be highly resistant to Picloram and Dicamba. Inter-generic hybrids between wild mustard and radish (Raphanus sativus, 2n = 18) were produced by traditional breeding coupled with in vitro embryo rescue/ovule culture. To increase frequency of embryo regeneration and hybrid plant production, several hundred reciprocal crosses were performed between these species. Upon altering cultural conditions and media composition, a high frequency of embryo regeneration and hybrid plant establishment was achieved. A protocol was also optimized for in vitro clonal multiplication of inter-generic hybrids produced by embryo rescue. To evaluate transfer of auxinic herbicide resistance from wild mustard into hybrid plants, several screening tests (involving in vitro, molecular-based as well as whole plant-based tests) were performed. Results indicated that hybrids of R. sativus x S. arevensis were resistant to auxinic herbicides suggesting, that, the resistance trait was transferred to these hybrids from the wild mustard. This research for the first time demonstrates the possibility of transfer of auxinic herbicide resistance from wild mustard to radish.
format	article
author	J Mithila J Christopher Hall
author_facet	J Mithila J Christopher Hall
author_sort	J Mithila
title	Transfer of Auxinic Herbicide Resistance from Wild Mustard (Sinapis arvensis) into Radish (Raphanus sativus) through Embryo Rescue
title_short	Transfer of Auxinic Herbicide Resistance from Wild Mustard (Sinapis arvensis) into Radish (Raphanus sativus) through Embryo Rescue
title_full	Transfer of Auxinic Herbicide Resistance from Wild Mustard (Sinapis arvensis) into Radish (Raphanus sativus) through Embryo Rescue
title_fullStr	Transfer of Auxinic Herbicide Resistance from Wild Mustard (Sinapis arvensis) into Radish (Raphanus sativus) through Embryo Rescue
title_full_unstemmed	Transfer of Auxinic Herbicide Resistance from Wild Mustard (Sinapis arvensis) into Radish (Raphanus sativus) through Embryo Rescue
title_sort	transfer of auxinic herbicide resistance from wild mustard (sinapis arvensis) into radish (raphanus sativus) through embryo rescue
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2012
url	https://doaj.org/article/df732f144f4d42d4a913b851173a176a
work_keys_str_mv	AT jmithila transferofauxinicherbicideresistancefromwildmustardsinapisarvensisintoradishraphanussativusthroughembryorescue AT jchristopherhall transferofauxinicherbicideresistancefromwildmustardsinapisarvensisintoradishraphanussativusthroughembryorescue
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Transfer of Auxinic Herbicide Resistance from Wild Mustard (Sinapis arvensis) into Radish (Raphanus sativus) through Embryo Rescue

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