Monitoring voltage fluctuations of intracellular membranes

Abstract In eukaryotic cells, the endoplasmic reticulum (ER) is the largest continuous membrane-enclosed network which surrounds a single lumen. Using a new genetically encoded voltage indicator (GEVI), we applied the patch clamp technique to cultured HEK293 cells and neurons and found that there is...

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Autores principales:	Masoud Sepehri Rad, Lawrence B. Cohen, Oliver Braubach, Bradley J. Baker
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/df74463288e042e4a8fca2dbb1150436
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spelling	oai:doaj.org-article:df74463288e042e4a8fca2dbb11504362021-12-02T11:41:14ZMonitoring voltage fluctuations of intracellular membranes10.1038/s41598-018-25083-72045-2322https://doaj.org/article/df74463288e042e4a8fca2dbb11504362018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25083-7https://doaj.org/toc/2045-2322Abstract In eukaryotic cells, the endoplasmic reticulum (ER) is the largest continuous membrane-enclosed network which surrounds a single lumen. Using a new genetically encoded voltage indicator (GEVI), we applied the patch clamp technique to cultured HEK293 cells and neurons and found that there is a very fast electrical interaction between the plasma membrane and internal membrane(s). This discovery suggests a novel mechanism for interaction between the external membrane and internal membranes as well as mechanisms for interactions between the various internal membranes. The ER may transfer electrical signals between the plasma membrane and other internal organelles. The internal membrane optical signal is reversed in polarity but has a time course similar to that of the plasma membrane signal. The optical signal of the GEVI in the plasma membrane is consistent from trial to trial. However, the internal signal decreases in size with repeated trials suggesting that the electrical coupling is degrading and/or the resistance of the internal membrane is decaying.Masoud Sepehri RadLawrence B. CohenOliver BraubachBradley J. BakerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Masoud Sepehri Rad Lawrence B. Cohen Oliver Braubach Bradley J. Baker Monitoring voltage fluctuations of intracellular membranes
description	Abstract In eukaryotic cells, the endoplasmic reticulum (ER) is the largest continuous membrane-enclosed network which surrounds a single lumen. Using a new genetically encoded voltage indicator (GEVI), we applied the patch clamp technique to cultured HEK293 cells and neurons and found that there is a very fast electrical interaction between the plasma membrane and internal membrane(s). This discovery suggests a novel mechanism for interaction between the external membrane and internal membranes as well as mechanisms for interactions between the various internal membranes. The ER may transfer electrical signals between the plasma membrane and other internal organelles. The internal membrane optical signal is reversed in polarity but has a time course similar to that of the plasma membrane signal. The optical signal of the GEVI in the plasma membrane is consistent from trial to trial. However, the internal signal decreases in size with repeated trials suggesting that the electrical coupling is degrading and/or the resistance of the internal membrane is decaying.
format	article
author	Masoud Sepehri Rad Lawrence B. Cohen Oliver Braubach Bradley J. Baker
author_facet	Masoud Sepehri Rad Lawrence B. Cohen Oliver Braubach Bradley J. Baker
author_sort	Masoud Sepehri Rad
title	Monitoring voltage fluctuations of intracellular membranes
title_short	Monitoring voltage fluctuations of intracellular membranes
title_full	Monitoring voltage fluctuations of intracellular membranes
title_fullStr	Monitoring voltage fluctuations of intracellular membranes
title_full_unstemmed	Monitoring voltage fluctuations of intracellular membranes
title_sort	monitoring voltage fluctuations of intracellular membranes
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/df74463288e042e4a8fca2dbb1150436
work_keys_str_mv	AT masoudsepehrirad monitoringvoltagefluctuationsofintracellularmembranes AT lawrencebcohen monitoringvoltagefluctuationsofintracellularmembranes AT oliverbraubach monitoringvoltagefluctuationsofintracellularmembranes AT bradleyjbaker monitoringvoltagefluctuationsofintracellularmembranes
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Monitoring voltage fluctuations of intracellular membranes

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