Immature CD4+CD8+ thymocytes are preferentially infected by measles virus in human thymic organ cultures.

Cells of the human immune system are important target cells for measles virus (MeV) infection and infection of these cells may contribute to the immunologic abnormalities and immune suppression that characterize measles. The thymus is the site for production of naïve T lymphocytes and is infected du...

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Autores principales:	Yukari Okamoto, Luca A Vricella, William J Moss, Diane E Griffin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/df76a3f449f04cd98b919c8cc76f61b8
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spelling	oai:doaj.org-article:df76a3f449f04cd98b919c8cc76f61b82021-11-18T07:04:17ZImmature CD4+CD8+ thymocytes are preferentially infected by measles virus in human thymic organ cultures.1932-620310.1371/journal.pone.0045999https://doaj.org/article/df76a3f449f04cd98b919c8cc76f61b82012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23029357/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Cells of the human immune system are important target cells for measles virus (MeV) infection and infection of these cells may contribute to the immunologic abnormalities and immune suppression that characterize measles. The thymus is the site for production of naïve T lymphocytes and is infected during measles. To determine which populations of thymocytes are susceptible to MeV infection and whether strains of MeV differ in their ability to infect thymocytes, we used ex vivo human thymus organ cultures to assess the relative susceptibility of different subpopulations of thymocytes to infection with wild type and vaccine strains of MeV. Thymocytes were susceptible to MeV infection with the most replication in immature CD4(+)CD8(+) double positive cells. Susceptibility correlated with the level of expression of the MeV receptor CD150. Wild type strains of MeV infected thymocytes more efficiently than the Edmonston vaccine strain. Thymus cultures from children ≥3 years of age were less susceptible to MeV infection than cultures from children 5 to 15 months of age. Resistance in one 7 year-old child was associated with production of interferon-gamma suggesting that vaccination may result in MeV-specific memory T cells in the thymus. We conclude that immature thymocytes are susceptible to MeV infection and thymocyte infection may contribute to the immunologic abnormalities associated with measles.Yukari OkamotoLuca A VricellaWilliam J MossDiane E GriffinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e45999 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yukari Okamoto Luca A Vricella William J Moss Diane E Griffin Immature CD4+CD8+ thymocytes are preferentially infected by measles virus in human thymic organ cultures.
description	Cells of the human immune system are important target cells for measles virus (MeV) infection and infection of these cells may contribute to the immunologic abnormalities and immune suppression that characterize measles. The thymus is the site for production of naïve T lymphocytes and is infected during measles. To determine which populations of thymocytes are susceptible to MeV infection and whether strains of MeV differ in their ability to infect thymocytes, we used ex vivo human thymus organ cultures to assess the relative susceptibility of different subpopulations of thymocytes to infection with wild type and vaccine strains of MeV. Thymocytes were susceptible to MeV infection with the most replication in immature CD4(+)CD8(+) double positive cells. Susceptibility correlated with the level of expression of the MeV receptor CD150. Wild type strains of MeV infected thymocytes more efficiently than the Edmonston vaccine strain. Thymus cultures from children ≥3 years of age were less susceptible to MeV infection than cultures from children 5 to 15 months of age. Resistance in one 7 year-old child was associated with production of interferon-gamma suggesting that vaccination may result in MeV-specific memory T cells in the thymus. We conclude that immature thymocytes are susceptible to MeV infection and thymocyte infection may contribute to the immunologic abnormalities associated with measles.
format	article
author	Yukari Okamoto Luca A Vricella William J Moss Diane E Griffin
author_facet	Yukari Okamoto Luca A Vricella William J Moss Diane E Griffin
author_sort	Yukari Okamoto
title	Immature CD4+CD8+ thymocytes are preferentially infected by measles virus in human thymic organ cultures.
title_short	Immature CD4+CD8+ thymocytes are preferentially infected by measles virus in human thymic organ cultures.
title_full	Immature CD4+CD8+ thymocytes are preferentially infected by measles virus in human thymic organ cultures.
title_fullStr	Immature CD4+CD8+ thymocytes are preferentially infected by measles virus in human thymic organ cultures.
title_full_unstemmed	Immature CD4+CD8+ thymocytes are preferentially infected by measles virus in human thymic organ cultures.
title_sort	immature cd4+cd8+ thymocytes are preferentially infected by measles virus in human thymic organ cultures.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/df76a3f449f04cd98b919c8cc76f61b8
work_keys_str_mv	AT yukariokamoto immaturecd4cd8thymocytesarepreferentiallyinfectedbymeaslesvirusinhumanthymicorgancultures AT lucaavricella immaturecd4cd8thymocytesarepreferentiallyinfectedbymeaslesvirusinhumanthymicorgancultures AT williamjmoss immaturecd4cd8thymocytesarepreferentiallyinfectedbymeaslesvirusinhumanthymicorgancultures AT dianeegriffin immaturecd4cd8thymocytesarepreferentiallyinfectedbymeaslesvirusinhumanthymicorgancultures
_version_	1718424010756194304

Immature CD4+CD8+ thymocytes are preferentially infected by measles virus in human thymic organ cultures.

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