SERPINH1, Targeted by miR-29b, Modulated Proliferation and Migration of Human Retinal Endothelial Cells Under High Glucose Conditions

Lingfei Hu, Yinping Liu, Chaobing Wei, Huixiang Jin, Lixin Mei, Changfan Wu Department of Ophthalmology, Yijishan Hospital of Wannan Medical College, Wuhu, Anhui Province, People’s Republic of ChinaCorrespondence: Changfan Wu Tel +86- 13909632351Email wuchangfan1971@sohu.comAim: In the present study...

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Autores principales:	Hu L, Liu Y, Wei C, Jin H, Mei L, Wu C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	diabetic retinopathy bioinformatics analysis hrecs serpinh1 proliferation migration Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/df772e8d241c49da98e53c1897a7c2cd
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spelling	oai:doaj.org-article:df772e8d241c49da98e53c1897a7c2cd2021-12-02T14:51:49ZSERPINH1, Targeted by miR-29b, Modulated Proliferation and Migration of Human Retinal Endothelial Cells Under High Glucose Conditions1178-7007https://doaj.org/article/df772e8d241c49da98e53c1897a7c2cd2021-08-01T00:00:00Zhttps://www.dovepress.com/serpinh1-targeted-by-mir-29b-modulated-proliferation-and-migration-of--peer-reviewed-fulltext-article-DMSOhttps://doaj.org/toc/1178-7007Lingfei Hu, Yinping Liu, Chaobing Wei, Huixiang Jin, Lixin Mei, Changfan Wu Department of Ophthalmology, Yijishan Hospital of Wannan Medical College, Wuhu, Anhui Province, People’s Republic of ChinaCorrespondence: Changfan Wu Tel +86- 13909632351Email wuchangfan1971@sohu.comAim: In the present study, we performed bioinformatics studies and in vitro functional assays to explore the underlying role of serpin family H member 1 (SERPINH1) in the diabetic retinopathy.Methods: Common differentially expressed genes (DEGs) between diabetic retinal tissues and normal retinal tissues were analyzed using Gene Expression Omnibus (GEO) database. The proliferation and migration of human retinal endothelial cells (HRECs) was evaluated by MTS, EdU and wound healing assays, respectively; the miRNA and mRNAs expression levels of hub genes in HRECs were determined using quantitative real-time PCR (qRT-PCR). Protein levels were determined using a Western blot assay.Results: A total of 189 common DEGs were screened between two GEO datasets (GSE60436 and GSE94019), and ten potential hub genes that may link to the progression of diabetic retinopathy were detected. The qRT-PCR results showed that collagen, type I, alpha 1 (COL1A1), Collagen, type I, alpha 2 (COL1A2) and serpin family H member 1 (SERPINH1) mRNA expression levels were up-regulated in the HRECs after being exposed to high glucose for 48 h. Silence of SERPINH1 repressed the high glucose-induced increase in proliferation and migration of HRECs. SERPINH1 was a target of miR-29b and was suppressed by miR-29 in HRECs. SERPINH1 overexpression promoted HREC proliferation and migration. Furthermore, miR-29b suppressed HREC proliferation and migration under high-glucose stimulation, which was significantly attenuated by enforced expression of SERPINH1.Conclusion: In conclusion, by performing the integrated bioinformatics analysis, the present study suggested that 3 hub genes (COL1A1, COL1A2 and SERPINH1) may be associated with diabetic retinopathy pathophysiology. Further mechanistic studies indicated that miR-29b/SERPINH1 signaling participated in high glucose-induced enhancement in the proliferation and migration of HRECs.Keywords: diabetic retinopathy, bioinformatics analysis, HRECs, SERPINH1, proliferation, migrationHu LLiu YWei CJin HMei LWu CDove Medical Pressarticlediabetic retinopathybioinformatics analysishrecsserpinh1proliferationmigrationSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 14, Pp 3471-3483 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	diabetic retinopathy bioinformatics analysis hrecs serpinh1 proliferation migration Specialties of internal medicine RC581-951
spellingShingle	diabetic retinopathy bioinformatics analysis hrecs serpinh1 proliferation migration Specialties of internal medicine RC581-951 Hu L Liu Y Wei C Jin H Mei L Wu C SERPINH1, Targeted by miR-29b, Modulated Proliferation and Migration of Human Retinal Endothelial Cells Under High Glucose Conditions
description	Lingfei Hu, Yinping Liu, Chaobing Wei, Huixiang Jin, Lixin Mei, Changfan Wu Department of Ophthalmology, Yijishan Hospital of Wannan Medical College, Wuhu, Anhui Province, People’s Republic of ChinaCorrespondence: Changfan Wu Tel +86- 13909632351Email wuchangfan1971@sohu.comAim: In the present study, we performed bioinformatics studies and in vitro functional assays to explore the underlying role of serpin family H member 1 (SERPINH1) in the diabetic retinopathy.Methods: Common differentially expressed genes (DEGs) between diabetic retinal tissues and normal retinal tissues were analyzed using Gene Expression Omnibus (GEO) database. The proliferation and migration of human retinal endothelial cells (HRECs) was evaluated by MTS, EdU and wound healing assays, respectively; the miRNA and mRNAs expression levels of hub genes in HRECs were determined using quantitative real-time PCR (qRT-PCR). Protein levels were determined using a Western blot assay.Results: A total of 189 common DEGs were screened between two GEO datasets (GSE60436 and GSE94019), and ten potential hub genes that may link to the progression of diabetic retinopathy were detected. The qRT-PCR results showed that collagen, type I, alpha 1 (COL1A1), Collagen, type I, alpha 2 (COL1A2) and serpin family H member 1 (SERPINH1) mRNA expression levels were up-regulated in the HRECs after being exposed to high glucose for 48 h. Silence of SERPINH1 repressed the high glucose-induced increase in proliferation and migration of HRECs. SERPINH1 was a target of miR-29b and was suppressed by miR-29 in HRECs. SERPINH1 overexpression promoted HREC proliferation and migration. Furthermore, miR-29b suppressed HREC proliferation and migration under high-glucose stimulation, which was significantly attenuated by enforced expression of SERPINH1.Conclusion: In conclusion, by performing the integrated bioinformatics analysis, the present study suggested that 3 hub genes (COL1A1, COL1A2 and SERPINH1) may be associated with diabetic retinopathy pathophysiology. Further mechanistic studies indicated that miR-29b/SERPINH1 signaling participated in high glucose-induced enhancement in the proliferation and migration of HRECs.Keywords: diabetic retinopathy, bioinformatics analysis, HRECs, SERPINH1, proliferation, migration
format	article
author	Hu L Liu Y Wei C Jin H Mei L Wu C
author_facet	Hu L Liu Y Wei C Jin H Mei L Wu C
author_sort	Hu L
title	SERPINH1, Targeted by miR-29b, Modulated Proliferation and Migration of Human Retinal Endothelial Cells Under High Glucose Conditions
title_short	SERPINH1, Targeted by miR-29b, Modulated Proliferation and Migration of Human Retinal Endothelial Cells Under High Glucose Conditions
title_full	SERPINH1, Targeted by miR-29b, Modulated Proliferation and Migration of Human Retinal Endothelial Cells Under High Glucose Conditions
title_fullStr	SERPINH1, Targeted by miR-29b, Modulated Proliferation and Migration of Human Retinal Endothelial Cells Under High Glucose Conditions
title_full_unstemmed	SERPINH1, Targeted by miR-29b, Modulated Proliferation and Migration of Human Retinal Endothelial Cells Under High Glucose Conditions
title_sort	serpinh1, targeted by mir-29b, modulated proliferation and migration of human retinal endothelial cells under high glucose conditions
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/df772e8d241c49da98e53c1897a7c2cd
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SERPINH1, Targeted by miR-29b, Modulated Proliferation and Migration of Human Retinal Endothelial Cells Under High Glucose Conditions

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