Multilocus Sequence Typing Reveals both Shared and Unique Genotypes of Cryptococcus neoformans in Jiangxi Province, China

Abstract Cryptococcosis is a globally distributed infectious fungal disease. However, much remains unknown about its molecular epidemiology in many parts of the world. In this study, we analyzed 86 clinical Cryptococcus neoformans isolates from 14 regions in Jiangxi Province in south central China....

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Autores principales:	Yan-Hui Chen, Feng Yu, Ze-Yuan Bian, Jian-Ming Hong, Nan Zhang, Qiao-Shi Zhong, Ya-Ping Hang, Jianping Xu, Long-Hua Hu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/df7e58edbb514f0298cecd1ccbc6ffc6
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spelling	oai:doaj.org-article:df7e58edbb514f0298cecd1ccbc6ffc62021-12-02T11:40:17ZMultilocus Sequence Typing Reveals both Shared and Unique Genotypes of Cryptococcus neoformans in Jiangxi Province, China10.1038/s41598-018-20054-42045-2322https://doaj.org/article/df7e58edbb514f0298cecd1ccbc6ffc62018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-20054-4https://doaj.org/toc/2045-2322Abstract Cryptococcosis is a globally distributed infectious fungal disease. However, much remains unknown about its molecular epidemiology in many parts of the world. In this study, we analyzed 86 clinical Cryptococcus neoformans isolates from 14 regions in Jiangxi Province in south central China. Each isolate was from a different patient and 35 of the 86 (40.7%) patients were infected with HIV. All strains belonged to serotype A and mating type α (MATα). Genotyping based on DNA sequences at seven nuclear loci revealed eight sequence types (STs) among the 86 isolates, including two novel STs that have not been reported from other parts of the world. ST5 was the dominant genotype and our comparative analyses showed that these genotypes in Jiangxi likely originated by dispersal from other regions within and outside of China and/or mutations from another genotype within Jiangxi. Though none of the isolates was resistant to the five tested antifungal drugs (flucytosine, amphotericin B, fluconazole, itraconazole, and voriconazole), obvious differences in their minimum inhibitory concentrations were observed, even among isolates of the same ST. Our results suggest that continuous monitoring should be conducted to understand the changing dynamics of C. neoformans in this and other regions.Yan-Hui ChenFeng YuZe-Yuan BianJian-Ming HongNan ZhangQiao-Shi ZhongYa-Ping HangJianping XuLong-Hua HuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yan-Hui Chen Feng Yu Ze-Yuan Bian Jian-Ming Hong Nan Zhang Qiao-Shi Zhong Ya-Ping Hang Jianping Xu Long-Hua Hu Multilocus Sequence Typing Reveals both Shared and Unique Genotypes of Cryptococcus neoformans in Jiangxi Province, China
description	Abstract Cryptococcosis is a globally distributed infectious fungal disease. However, much remains unknown about its molecular epidemiology in many parts of the world. In this study, we analyzed 86 clinical Cryptococcus neoformans isolates from 14 regions in Jiangxi Province in south central China. Each isolate was from a different patient and 35 of the 86 (40.7%) patients were infected with HIV. All strains belonged to serotype A and mating type α (MATα). Genotyping based on DNA sequences at seven nuclear loci revealed eight sequence types (STs) among the 86 isolates, including two novel STs that have not been reported from other parts of the world. ST5 was the dominant genotype and our comparative analyses showed that these genotypes in Jiangxi likely originated by dispersal from other regions within and outside of China and/or mutations from another genotype within Jiangxi. Though none of the isolates was resistant to the five tested antifungal drugs (flucytosine, amphotericin B, fluconazole, itraconazole, and voriconazole), obvious differences in their minimum inhibitory concentrations were observed, even among isolates of the same ST. Our results suggest that continuous monitoring should be conducted to understand the changing dynamics of C. neoformans in this and other regions.
format	article
author	Yan-Hui Chen Feng Yu Ze-Yuan Bian Jian-Ming Hong Nan Zhang Qiao-Shi Zhong Ya-Ping Hang Jianping Xu Long-Hua Hu
author_facet	Yan-Hui Chen Feng Yu Ze-Yuan Bian Jian-Ming Hong Nan Zhang Qiao-Shi Zhong Ya-Ping Hang Jianping Xu Long-Hua Hu
author_sort	Yan-Hui Chen
title	Multilocus Sequence Typing Reveals both Shared and Unique Genotypes of Cryptococcus neoformans in Jiangxi Province, China
title_short	Multilocus Sequence Typing Reveals both Shared and Unique Genotypes of Cryptococcus neoformans in Jiangxi Province, China
title_full	Multilocus Sequence Typing Reveals both Shared and Unique Genotypes of Cryptococcus neoformans in Jiangxi Province, China
title_fullStr	Multilocus Sequence Typing Reveals both Shared and Unique Genotypes of Cryptococcus neoformans in Jiangxi Province, China
title_full_unstemmed	Multilocus Sequence Typing Reveals both Shared and Unique Genotypes of Cryptococcus neoformans in Jiangxi Province, China
title_sort	multilocus sequence typing reveals both shared and unique genotypes of cryptococcus neoformans in jiangxi province, china
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/df7e58edbb514f0298cecd1ccbc6ffc6
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Multilocus Sequence Typing Reveals both Shared and Unique Genotypes of Cryptococcus neoformans in Jiangxi Province, China

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