Mouse papillomavirus infection persists in mucosal tissues of an immunocompetent mouse strain and progresses to cancer

Abstract Mouse papillomavirus has shown broad tissue tropism in nude mice. Previous studies have tested cutaneous infections in different immunocompromised and immunocompetent mouse strains. In the current study, we examined mucosal infection in several immunocompetent and immunocompromised mouse st...

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Autores principales:	Nancy M. Cladel, Lynn R. Budgeon, Karla K. Balogh, Timothy K. Cooper, Sarah A. Brendle, Neil D. Christensen, Todd D. Schell, Jiafen Hu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/df8831acce684d48b751234e509a3a56
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spelling	oai:doaj.org-article:df8831acce684d48b751234e509a3a562021-12-02T15:05:55ZMouse papillomavirus infection persists in mucosal tissues of an immunocompetent mouse strain and progresses to cancer10.1038/s41598-017-17089-42045-2322https://doaj.org/article/df8831acce684d48b751234e509a3a562017-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-17089-4https://doaj.org/toc/2045-2322Abstract Mouse papillomavirus has shown broad tissue tropism in nude mice. Previous studies have tested cutaneous infections in different immunocompromised and immunocompetent mouse strains. In the current study, we examined mucosal infection in several immunocompetent and immunocompromised mouse strains. Viral DNA was monitored periodically by Q-PCR of lavage samples. Immunohistochemistry and in situ hybridization were used to determine viral capsid protein and viral DNA respectively. All athymic nude mouse strains showed active infections at both cutaneous and mucosal sites. Interestingly, NOD/SCID mice, which have a deficiency in T, B, and NK cells, showed minimal disease at cutaneous sites but developed persistent infection at the mucosal sites including those of the anogenital region and the oral cavity. Three strains of immunocompetent mice supported mucosal infections. Infections of the lower genital tract in heterozygous (immunocompetent) mice of the NU/J strain progressed to high grade dysplasia and to carcinoma in situ. Anti-MmuPV1 neutralizing antibodies were detected in the sera of all immunocompetent animals. Our findings demonstrate that the mucosae may be the preferred sites for this virus in mice. The mouse model is expected to be a valuable model for the study of mucosal papillomavirus disease, progression, and host immune control.Nancy M. CladelLynn R. BudgeonKarla K. BaloghTimothy K. CooperSarah A. BrendleNeil D. ChristensenTodd D. SchellJiafen HuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nancy M. Cladel Lynn R. Budgeon Karla K. Balogh Timothy K. Cooper Sarah A. Brendle Neil D. Christensen Todd D. Schell Jiafen Hu Mouse papillomavirus infection persists in mucosal tissues of an immunocompetent mouse strain and progresses to cancer
description	Abstract Mouse papillomavirus has shown broad tissue tropism in nude mice. Previous studies have tested cutaneous infections in different immunocompromised and immunocompetent mouse strains. In the current study, we examined mucosal infection in several immunocompetent and immunocompromised mouse strains. Viral DNA was monitored periodically by Q-PCR of lavage samples. Immunohistochemistry and in situ hybridization were used to determine viral capsid protein and viral DNA respectively. All athymic nude mouse strains showed active infections at both cutaneous and mucosal sites. Interestingly, NOD/SCID mice, which have a deficiency in T, B, and NK cells, showed minimal disease at cutaneous sites but developed persistent infection at the mucosal sites including those of the anogenital region and the oral cavity. Three strains of immunocompetent mice supported mucosal infections. Infections of the lower genital tract in heterozygous (immunocompetent) mice of the NU/J strain progressed to high grade dysplasia and to carcinoma in situ. Anti-MmuPV1 neutralizing antibodies were detected in the sera of all immunocompetent animals. Our findings demonstrate that the mucosae may be the preferred sites for this virus in mice. The mouse model is expected to be a valuable model for the study of mucosal papillomavirus disease, progression, and host immune control.
format	article
author	Nancy M. Cladel Lynn R. Budgeon Karla K. Balogh Timothy K. Cooper Sarah A. Brendle Neil D. Christensen Todd D. Schell Jiafen Hu
author_facet	Nancy M. Cladel Lynn R. Budgeon Karla K. Balogh Timothy K. Cooper Sarah A. Brendle Neil D. Christensen Todd D. Schell Jiafen Hu
author_sort	Nancy M. Cladel
title	Mouse papillomavirus infection persists in mucosal tissues of an immunocompetent mouse strain and progresses to cancer
title_short	Mouse papillomavirus infection persists in mucosal tissues of an immunocompetent mouse strain and progresses to cancer
title_full	Mouse papillomavirus infection persists in mucosal tissues of an immunocompetent mouse strain and progresses to cancer
title_fullStr	Mouse papillomavirus infection persists in mucosal tissues of an immunocompetent mouse strain and progresses to cancer
title_full_unstemmed	Mouse papillomavirus infection persists in mucosal tissues of an immunocompetent mouse strain and progresses to cancer
title_sort	mouse papillomavirus infection persists in mucosal tissues of an immunocompetent mouse strain and progresses to cancer
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/df8831acce684d48b751234e509a3a56
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Mouse papillomavirus infection persists in mucosal tissues of an immunocompetent mouse strain and progresses to cancer

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