Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging

Fluorescent reporters spanning the visible spectrum are needed for imaging live cells and organisms. Here the authors report a collection of fluorogenic chromophores that cover the visible spectrum from blue to red using a single engineered and optimised protein tag.

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Autores principales: Hela Benaissa, Karim Ounoughi, Isabelle Aujard, Evelyne Fischer, Rosette Goïame, Julie Nguyen, Alison G. Tebo, Chenge Li, Thomas Le Saux, Giulia Bertolin, Marc Tramier, Lydia Danglot, Nicolas Pietrancosta, Xavier Morin, Ludovic Jullien, Arnaud Gautier
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/df8876daa7ff41be9c6ff80c673882dd
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spelling oai:doaj.org-article:df8876daa7ff41be9c6ff80c673882dd2021-12-05T12:21:57ZEngineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging10.1038/s41467-021-27334-02041-1723https://doaj.org/article/df8876daa7ff41be9c6ff80c673882dd2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-27334-0https://doaj.org/toc/2041-1723Fluorescent reporters spanning the visible spectrum are needed for imaging live cells and organisms. Here the authors report a collection of fluorogenic chromophores that cover the visible spectrum from blue to red using a single engineered and optimised protein tag.Hela BenaissaKarim OunoughiIsabelle AujardEvelyne FischerRosette GoïameJulie NguyenAlison G. TeboChenge LiThomas Le SauxGiulia BertolinMarc TramierLydia DanglotNicolas PietrancostaXavier MorinLudovic JullienArnaud GautierNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-17 (2021)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Hela Benaissa
Karim Ounoughi
Isabelle Aujard
Evelyne Fischer
Rosette Goïame
Julie Nguyen
Alison G. Tebo
Chenge Li
Thomas Le Saux
Giulia Bertolin
Marc Tramier
Lydia Danglot
Nicolas Pietrancosta
Xavier Morin
Ludovic Jullien
Arnaud Gautier
Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging
description Fluorescent reporters spanning the visible spectrum are needed for imaging live cells and organisms. Here the authors report a collection of fluorogenic chromophores that cover the visible spectrum from blue to red using a single engineered and optimised protein tag.
format article
author Hela Benaissa
Karim Ounoughi
Isabelle Aujard
Evelyne Fischer
Rosette Goïame
Julie Nguyen
Alison G. Tebo
Chenge Li
Thomas Le Saux
Giulia Bertolin
Marc Tramier
Lydia Danglot
Nicolas Pietrancosta
Xavier Morin
Ludovic Jullien
Arnaud Gautier
author_facet Hela Benaissa
Karim Ounoughi
Isabelle Aujard
Evelyne Fischer
Rosette Goïame
Julie Nguyen
Alison G. Tebo
Chenge Li
Thomas Le Saux
Giulia Bertolin
Marc Tramier
Lydia Danglot
Nicolas Pietrancosta
Xavier Morin
Ludovic Jullien
Arnaud Gautier
author_sort Hela Benaissa
title Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging
title_short Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging
title_full Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging
title_fullStr Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging
title_full_unstemmed Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging
title_sort engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/df8876daa7ff41be9c6ff80c673882dd
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