CO<sub>2</sub> Signaling through the Ptc2-Ssn3 Axis Governs Sustained Hyphal Development of <named-content content-type="genus-species">Candida albicans</named-content> by Reducing Ume6 Phosphorylation and Degradation

ABSTRACT Candida albicans is the most common cause of invasive fungal infections in humans. Its ability to sense and adapt to changing carbon dioxide levels is crucial for its pathogenesis. Carbon dioxide promotes hyphal development. The hypha-specific transcription factor Ume6 is rapidly degraded i...

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Autores principales:	Yang Lu, Chang Su, Shatarupa Ray, Yuncong Yuan, Haoping Liu
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	Candida albicans Ssn3/Cdk8 Ume6 carbon dioxide signaling hyphal development Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/df8a98c13a264c1b912927001b3d761a
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spelling	oai:doaj.org-article:df8a98c13a264c1b912927001b3d761a2021-11-15T15:55:14ZCO<sub>2</sub> Signaling through the Ptc2-Ssn3 Axis Governs Sustained Hyphal Development of <named-content content-type="genus-species">Candida albicans</named-content> by Reducing Ume6 Phosphorylation and Degradation10.1128/mBio.02320-182150-7511https://doaj.org/article/df8a98c13a264c1b912927001b3d761a2019-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02320-18https://doaj.org/toc/2150-7511ABSTRACT Candida albicans is the most common cause of invasive fungal infections in humans. Its ability to sense and adapt to changing carbon dioxide levels is crucial for its pathogenesis. Carbon dioxide promotes hyphal development. The hypha-specific transcription factor Ume6 is rapidly degraded in air, but is stable under physiological CO2 and hypoxia to sustain hyphal elongation. Here, we show that Ume6 stability is regulated by two parallel E3 ubiquitin ligases, SCFGrr1 and Ubr1, in response to CO2 and O2, respectively. To uncover the CO2 signaling pathway that regulates Ume6 stability, we performed genetic screens for mutants unable to respond to CO2 for sustained filamentation. We find that the type 2C protein phosphatase Ptc2 is specifically required for CO2-induced stabilization of Ume6 and hyphal elongation. In contrast, the cyclin-dependent kinase Ssn3 is found to be required for Ume6 phosphorylation and degradation in atmospheric CO2. Furthermore, we find that Ssn3 is dephosphorylated in 5% CO2 in a Ptc2-dependent manner, whereas deletion of PTC2 has no effect on Ssn3 phosphorylation in air. Our study uncovers the Ptc2-Ssn3 axis as a new CO2 signaling pathway that controls hyphal elongation by regulating Ume6 stability in C. albicans. IMPORTANCE The capacity to sense and adapt to changing carbon dioxide levels is crucial for all organisms. In fungi, CO2 is a key determinant involved in fundamental biological processes, including growth, morphology, and virulence. In the pathogenic fungus Candida albicans, high CO2 is directly sensed by adenylyl cyclase to promote hyphal growth. However, little is known about the mechanism by which hyphal development is maintained in response to physiological levels of CO2. Here we report that a signal transduction system mediated by a phosphatase-kinase pair controls CO2-responsive Ume6 phosphorylation and stability that in turn dictate hyphal elongation. Our results unravel a new regulatory mechanism of CO2 signaling in fungi.Yang LuChang SuShatarupa RayYuncong YuanHaoping LiuAmerican Society for MicrobiologyarticleCandida albicansSsn3/Cdk8Ume6carbon dioxide signalinghyphal developmentMicrobiologyQR1-502ENmBio, Vol 10, Iss 1 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Candida albicans Ssn3/Cdk8 Ume6 carbon dioxide signaling hyphal development Microbiology QR1-502
spellingShingle	Candida albicans Ssn3/Cdk8 Ume6 carbon dioxide signaling hyphal development Microbiology QR1-502 Yang Lu Chang Su Shatarupa Ray Yuncong Yuan Haoping Liu CO<sub>2</sub> Signaling through the Ptc2-Ssn3 Axis Governs Sustained Hyphal Development of <named-content content-type="genus-species">Candida albicans</named-content> by Reducing Ume6 Phosphorylation and Degradation
description	ABSTRACT Candida albicans is the most common cause of invasive fungal infections in humans. Its ability to sense and adapt to changing carbon dioxide levels is crucial for its pathogenesis. Carbon dioxide promotes hyphal development. The hypha-specific transcription factor Ume6 is rapidly degraded in air, but is stable under physiological CO2 and hypoxia to sustain hyphal elongation. Here, we show that Ume6 stability is regulated by two parallel E3 ubiquitin ligases, SCFGrr1 and Ubr1, in response to CO2 and O2, respectively. To uncover the CO2 signaling pathway that regulates Ume6 stability, we performed genetic screens for mutants unable to respond to CO2 for sustained filamentation. We find that the type 2C protein phosphatase Ptc2 is specifically required for CO2-induced stabilization of Ume6 and hyphal elongation. In contrast, the cyclin-dependent kinase Ssn3 is found to be required for Ume6 phosphorylation and degradation in atmospheric CO2. Furthermore, we find that Ssn3 is dephosphorylated in 5% CO2 in a Ptc2-dependent manner, whereas deletion of PTC2 has no effect on Ssn3 phosphorylation in air. Our study uncovers the Ptc2-Ssn3 axis as a new CO2 signaling pathway that controls hyphal elongation by regulating Ume6 stability in C. albicans. IMPORTANCE The capacity to sense and adapt to changing carbon dioxide levels is crucial for all organisms. In fungi, CO2 is a key determinant involved in fundamental biological processes, including growth, morphology, and virulence. In the pathogenic fungus Candida albicans, high CO2 is directly sensed by adenylyl cyclase to promote hyphal growth. However, little is known about the mechanism by which hyphal development is maintained in response to physiological levels of CO2. Here we report that a signal transduction system mediated by a phosphatase-kinase pair controls CO2-responsive Ume6 phosphorylation and stability that in turn dictate hyphal elongation. Our results unravel a new regulatory mechanism of CO2 signaling in fungi.
format	article
author	Yang Lu Chang Su Shatarupa Ray Yuncong Yuan Haoping Liu
author_facet	Yang Lu Chang Su Shatarupa Ray Yuncong Yuan Haoping Liu
author_sort	Yang Lu
title	CO<sub>2</sub> Signaling through the Ptc2-Ssn3 Axis Governs Sustained Hyphal Development of <named-content content-type="genus-species">Candida albicans</named-content> by Reducing Ume6 Phosphorylation and Degradation
title_short	CO<sub>2</sub> Signaling through the Ptc2-Ssn3 Axis Governs Sustained Hyphal Development of <named-content content-type="genus-species">Candida albicans</named-content> by Reducing Ume6 Phosphorylation and Degradation
title_full	CO<sub>2</sub> Signaling through the Ptc2-Ssn3 Axis Governs Sustained Hyphal Development of <named-content content-type="genus-species">Candida albicans</named-content> by Reducing Ume6 Phosphorylation and Degradation
title_fullStr	CO<sub>2</sub> Signaling through the Ptc2-Ssn3 Axis Governs Sustained Hyphal Development of <named-content content-type="genus-species">Candida albicans</named-content> by Reducing Ume6 Phosphorylation and Degradation
title_full_unstemmed	CO<sub>2</sub> Signaling through the Ptc2-Ssn3 Axis Governs Sustained Hyphal Development of <named-content content-type="genus-species">Candida albicans</named-content> by Reducing Ume6 Phosphorylation and Degradation
title_sort	co<sub>2</sub> signaling through the ptc2-ssn3 axis governs sustained hyphal development of <named-content content-type="genus-species">candida albicans</named-content> by reducing ume6 phosphorylation and degradation
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/df8a98c13a264c1b912927001b3d761a
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CO<sub>2</sub> Signaling through the Ptc2-Ssn3 Axis Governs Sustained Hyphal Development of <named-content content-type="genus-species">Candida albicans</named-content> by Reducing Ume6 Phosphorylation and Degradation

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