Non-conditioned bone marrow chimeric mouse generation using culture-based enrichment of hematopoietic stem and progenitor cells

Non-conditioned bone marrow chimeric mouse generation using culture-based enrichment of hematopoietic stem and progenitor cells

Bone marrow chimaeric mice are a valuable tool in research, but require myeloablative conditioning. Here the authors demonstrate efficient FACS-free enrichment of haematopoietic stem and progenitor cells for transplantation into unconditioned recipient mice, as well as for genetic engineering using...

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Autores principales: Kiyosumi Ochi, Maiko Morita, Adam C. Wilkinson, Atsushi Iwama, Satoshi Yamazaki
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/df8da65f907e470fb2d843f89f224190
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spelling oai:doaj.org-article:df8da65f907e470fb2d843f89f2241902021-12-02T17:38:25ZNon-conditioned bone marrow chimeric mouse generation using culture-based enrichment of hematopoietic stem and progenitor cells10.1038/s41467-021-23763-z2041-1723https://doaj.org/article/df8da65f907e470fb2d843f89f2241902021-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-23763-zhttps://doaj.org/toc/2041-1723Bone marrow chimaeric mice are a valuable tool in research, but require myeloablative conditioning. Here the authors demonstrate efficient FACS-free enrichment of haematopoietic stem and progenitor cells for transplantation into unconditioned recipient mice, as well as for genetic engineering using polyvinyl alcohol based media.Kiyosumi OchiMaiko MoritaAdam C. WilkinsonAtsushi IwamaSatoshi YamazakiNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Kiyosumi Ochi
Maiko Morita
Adam C. Wilkinson
Atsushi Iwama
Satoshi Yamazaki
Non-conditioned bone marrow chimeric mouse generation using culture-based enrichment of hematopoietic stem and progenitor cells
description Bone marrow chimaeric mice are a valuable tool in research, but require myeloablative conditioning. Here the authors demonstrate efficient FACS-free enrichment of haematopoietic stem and progenitor cells for transplantation into unconditioned recipient mice, as well as for genetic engineering using polyvinyl alcohol based media.
format article
author Kiyosumi Ochi
Maiko Morita
Adam C. Wilkinson
Atsushi Iwama
Satoshi Yamazaki
author_facet Kiyosumi Ochi
Maiko Morita
Adam C. Wilkinson
Atsushi Iwama
Satoshi Yamazaki
author_sort Kiyosumi Ochi
title Non-conditioned bone marrow chimeric mouse generation using culture-based enrichment of hematopoietic stem and progenitor cells
title_short Non-conditioned bone marrow chimeric mouse generation using culture-based enrichment of hematopoietic stem and progenitor cells
title_full Non-conditioned bone marrow chimeric mouse generation using culture-based enrichment of hematopoietic stem and progenitor cells
title_fullStr Non-conditioned bone marrow chimeric mouse generation using culture-based enrichment of hematopoietic stem and progenitor cells
title_full_unstemmed Non-conditioned bone marrow chimeric mouse generation using culture-based enrichment of hematopoietic stem and progenitor cells
title_sort non-conditioned bone marrow chimeric mouse generation using culture-based enrichment of hematopoietic stem and progenitor cells
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/df8da65f907e470fb2d843f89f224190
work_keys_str_mv AT kiyosumiochi nonconditionedbonemarrowchimericmousegenerationusingculturebasedenrichmentofhematopoieticstemandprogenitorcells
AT maikomorita nonconditionedbonemarrowchimericmousegenerationusingculturebasedenrichmentofhematopoieticstemandprogenitorcells
AT adamcwilkinson nonconditionedbonemarrowchimericmousegenerationusingculturebasedenrichmentofhematopoieticstemandprogenitorcells
AT atsushiiwama nonconditionedbonemarrowchimericmousegenerationusingculturebasedenrichmentofhematopoieticstemandprogenitorcells
AT satoshiyamazaki nonconditionedbonemarrowchimericmousegenerationusingculturebasedenrichmentofhematopoieticstemandprogenitorcells
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