Light-triggered liposomal cargo delivery platform incorporating photosensitizers and gold nanoparticles for enhanced singlet oxygen generation and increased cytotoxicity

Zofia Kautzka, Sandhya Clement, Ewa M Goldys, Wei Deng Centre of Excellence for Nanoscale BioPhotonics, Macquarie University, North Ryde, Sydney, NSW, Australia Abstract: We developed light-triggered liposomes incorporating 3–5 nm hydrophobic gold nanoparticles and Rose Bengal (...

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Autores principales:	Kautzka Z, Clement S, Goldys EM, Deng W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Liposomes Rose Bengal Gold nanoparticles Singlet oxygen generation Photodynamic therapy Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/df9051e1a5fc4cc083edf5abdceafeaa
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spelling	oai:doaj.org-article:df9051e1a5fc4cc083edf5abdceafeaa2021-12-02T08:11:16ZLight-triggered liposomal cargo delivery platform incorporating photosensitizers and gold nanoparticles for enhanced singlet oxygen generation and increased cytotoxicity1178-2013https://doaj.org/article/df9051e1a5fc4cc083edf5abdceafeaa2017-02-01T00:00:00Zhttps://www.dovepress.com/light-triggered-liposomal-cargo-delivery-platform-incorporating-photos-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Zofia Kautzka, Sandhya Clement, Ewa M Goldys, Wei Deng Centre of Excellence for Nanoscale BioPhotonics, Macquarie University, North Ryde, Sydney, NSW, Australia Abstract: We developed light-triggered liposomes incorporating 3–5 nm hydrophobic gold nanoparticles and Rose Bengal (RB), a well-known photosensitizer used for photodynamic therapy. Singlet oxygen generated by these liposomes with 532 nm light illumination was characterized for varying the molar ratio of lipids and gold nanoparticles while keeping the amount of RB constant. Gold nanoparticles were found to enhance the singlet oxygen generation rate, with a maximum enhancement factor of 1.75 obtained for the molar ratio of hydrogenated soy l-α-phosphatidylcholine:1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(hexanoylamine):gold of 57:5:17 compared with liposomes loaded with RB alone. The experimental results could be explained by the local electric field enhancement caused by gold nanoparticles. We further assessed cellular cytotoxicity of gold-loaded liposomes by encapsulating an antitumor drug, doxorubicin (Dox); such Dox-loaded liposomes were applied to human colorectal cancer cells (HCT116) and exposed to light. Gold-loaded liposomes containing RB and Dox where Dox release was triggered by light were found to exhibit higher cytotoxicity compared with the liposomes loaded with RB and Dox alone. Our results indicate that gold-loaded liposomes incorporating photosensitizers may serve as improved agents in photodynamic therapy and chemotherapy. Keywords: liposomes, Rose Bengal, gold nanoparticles, singlet oxygen generation, photodynamic therapyKautzka ZClement SGoldys EMDeng WDove Medical PressarticleLiposomesRose BengalGold nanoparticlesSinglet oxygen generationPhotodynamic therapyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 969-977 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Liposomes Rose Bengal Gold nanoparticles Singlet oxygen generation Photodynamic therapy Medicine (General) R5-920
spellingShingle	Liposomes Rose Bengal Gold nanoparticles Singlet oxygen generation Photodynamic therapy Medicine (General) R5-920 Kautzka Z Clement S Goldys EM Deng W Light-triggered liposomal cargo delivery platform incorporating photosensitizers and gold nanoparticles for enhanced singlet oxygen generation and increased cytotoxicity
description	Zofia Kautzka, Sandhya Clement, Ewa M Goldys, Wei Deng Centre of Excellence for Nanoscale BioPhotonics, Macquarie University, North Ryde, Sydney, NSW, Australia Abstract: We developed light-triggered liposomes incorporating 3–5 nm hydrophobic gold nanoparticles and Rose Bengal (RB), a well-known photosensitizer used for photodynamic therapy. Singlet oxygen generated by these liposomes with 532 nm light illumination was characterized for varying the molar ratio of lipids and gold nanoparticles while keeping the amount of RB constant. Gold nanoparticles were found to enhance the singlet oxygen generation rate, with a maximum enhancement factor of 1.75 obtained for the molar ratio of hydrogenated soy l-α-phosphatidylcholine:1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(hexanoylamine):gold of 57:5:17 compared with liposomes loaded with RB alone. The experimental results could be explained by the local electric field enhancement caused by gold nanoparticles. We further assessed cellular cytotoxicity of gold-loaded liposomes by encapsulating an antitumor drug, doxorubicin (Dox); such Dox-loaded liposomes were applied to human colorectal cancer cells (HCT116) and exposed to light. Gold-loaded liposomes containing RB and Dox where Dox release was triggered by light were found to exhibit higher cytotoxicity compared with the liposomes loaded with RB and Dox alone. Our results indicate that gold-loaded liposomes incorporating photosensitizers may serve as improved agents in photodynamic therapy and chemotherapy. Keywords: liposomes, Rose Bengal, gold nanoparticles, singlet oxygen generation, photodynamic therapy
format	article
author	Kautzka Z Clement S Goldys EM Deng W
author_facet	Kautzka Z Clement S Goldys EM Deng W
author_sort	Kautzka Z
title	Light-triggered liposomal cargo delivery platform incorporating photosensitizers and gold nanoparticles for enhanced singlet oxygen generation and increased cytotoxicity
title_short	Light-triggered liposomal cargo delivery platform incorporating photosensitizers and gold nanoparticles for enhanced singlet oxygen generation and increased cytotoxicity
title_full	Light-triggered liposomal cargo delivery platform incorporating photosensitizers and gold nanoparticles for enhanced singlet oxygen generation and increased cytotoxicity
title_fullStr	Light-triggered liposomal cargo delivery platform incorporating photosensitizers and gold nanoparticles for enhanced singlet oxygen generation and increased cytotoxicity
title_full_unstemmed	Light-triggered liposomal cargo delivery platform incorporating photosensitizers and gold nanoparticles for enhanced singlet oxygen generation and increased cytotoxicity
title_sort	light-triggered liposomal cargo delivery platform incorporating photosensitizers and gold nanoparticles for enhanced singlet oxygen generation and increased cytotoxicity
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/df9051e1a5fc4cc083edf5abdceafeaa
work_keys_str_mv	AT kautzkaz lighttriggeredliposomalcargodeliveryplatformincorporatingphotosensitizersandgoldnanoparticlesforenhancedsingletoxygengenerationandincreasedcytotoxicity AT clements lighttriggeredliposomalcargodeliveryplatformincorporatingphotosensitizersandgoldnanoparticlesforenhancedsingletoxygengenerationandincreasedcytotoxicity AT goldysem lighttriggeredliposomalcargodeliveryplatformincorporatingphotosensitizersandgoldnanoparticlesforenhancedsingletoxygengenerationandincreasedcytotoxicity AT dengw lighttriggeredliposomalcargodeliveryplatformincorporatingphotosensitizersandgoldnanoparticlesforenhancedsingletoxygengenerationandincreasedcytotoxicity
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Light-triggered liposomal cargo delivery platform incorporating photosensitizers and gold nanoparticles for enhanced singlet oxygen generation and increased cytotoxicity

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