Microbial dysbiosis in colorectal cancer (CRC) patients.

<h4>Unlabelled</h4>The composition of the human intestinal microbiota is linked to health status. The aim was to analyze the microbiota of normal and colon cancer patients in order to establish cancer-related dysbiosis.<h4>Patients and methods</h4>Stool bacterial DNA was extr...

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Autores principales:	Iradj Sobhani, Julien Tap, Françoise Roudot-Thoraval, Jean P Roperch, Sophie Letulle, Philippe Langella, Gérard Corthier, Jeanne Tran Van Nhieu, Jean P Furet
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/df92053d476b440095f4ffd4da31c1ca
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spelling	oai:doaj.org-article:df92053d476b440095f4ffd4da31c1ca2021-11-18T06:59:48ZMicrobial dysbiosis in colorectal cancer (CRC) patients.1932-620310.1371/journal.pone.0016393https://doaj.org/article/df92053d476b440095f4ffd4da31c1ca2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21297998/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Unlabelled</h4>The composition of the human intestinal microbiota is linked to health status. The aim was to analyze the microbiota of normal and colon cancer patients in order to establish cancer-related dysbiosis.<h4>Patients and methods</h4>Stool bacterial DNA was extracted prior to colonoscopy from 179 patients: 60 with colorectal cancer, and 119 with normal colonoscopy. Bacterial genes obtained by pyrosequencing of 12 stool samples (6 Normal and 6 Cancer) were subjected to a validated Principal Component Analysis (PCA) test. The dominant and subdominant bacterial population (C. leptum, C. coccoides, Bacteroides/Prevotella, Lactobacillus/Leuconostoc/Pediococcus groups, Bifidobacterium genus, and E. coli, and Faecalibacterium prausnitzii species) were quantified in all individuals using qPCR and specific IL17 producer cells in the intestinal mucosa were characterized using immunohistochemistry.<h4>Findings</h4>Pyrosequencing (Minimal sequence 200 nucleotide reads) revealed 80% of all sequences could be assigned to a total of 819 taxa based on default parameter of Classifier software. The phylogenetic core in Cancer individuals was different from that in Normal individuals according to the PCA analysis, with trends towards differences in the dominant and subdominant families of bacteria. Consequently, All-bacteria [log(10) (bacteria/g of stool)] in Normal, and Cancer individuals were similar [11.88±0.35, and 11.80±0.56, respectively, (P = 0.16)], according to qPCR values whereas among all dominant and subdominant species only those of Bacteroides/Prevotella were higher (All bacteria-specific bacterium; P = 0.009) in Cancer (-1.04±0.55) than in Normal (-1.40±0.83) individuals. IL17 immunoreactive cells were significantly expressed more in the normal mucosa of cancer patients than in those with normal colonoscopy.<h4>Conclusion</h4>This is the first large series to demonstrate a composition change in the microbiota of colon cancer patients with possible impact on mucosal immune response. These data open new filed for mass screening and pathophysiology investigations.Iradj SobhaniJulien TapFrançoise Roudot-ThoravalJean P RoperchSophie LetullePhilippe LangellaGérard CorthierJeanne Tran Van NhieuJean P FuretPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 1, p e16393 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Iradj Sobhani Julien Tap Françoise Roudot-Thoraval Jean P Roperch Sophie Letulle Philippe Langella Gérard Corthier Jeanne Tran Van Nhieu Jean P Furet Microbial dysbiosis in colorectal cancer (CRC) patients.
description	<h4>Unlabelled</h4>The composition of the human intestinal microbiota is linked to health status. The aim was to analyze the microbiota of normal and colon cancer patients in order to establish cancer-related dysbiosis.<h4>Patients and methods</h4>Stool bacterial DNA was extracted prior to colonoscopy from 179 patients: 60 with colorectal cancer, and 119 with normal colonoscopy. Bacterial genes obtained by pyrosequencing of 12 stool samples (6 Normal and 6 Cancer) were subjected to a validated Principal Component Analysis (PCA) test. The dominant and subdominant bacterial population (C. leptum, C. coccoides, Bacteroides/Prevotella, Lactobacillus/Leuconostoc/Pediococcus groups, Bifidobacterium genus, and E. coli, and Faecalibacterium prausnitzii species) were quantified in all individuals using qPCR and specific IL17 producer cells in the intestinal mucosa were characterized using immunohistochemistry.<h4>Findings</h4>Pyrosequencing (Minimal sequence 200 nucleotide reads) revealed 80% of all sequences could be assigned to a total of 819 taxa based on default parameter of Classifier software. The phylogenetic core in Cancer individuals was different from that in Normal individuals according to the PCA analysis, with trends towards differences in the dominant and subdominant families of bacteria. Consequently, All-bacteria [log(10) (bacteria/g of stool)] in Normal, and Cancer individuals were similar [11.88±0.35, and 11.80±0.56, respectively, (P = 0.16)], according to qPCR values whereas among all dominant and subdominant species only those of Bacteroides/Prevotella were higher (All bacteria-specific bacterium; P = 0.009) in Cancer (-1.04±0.55) than in Normal (-1.40±0.83) individuals. IL17 immunoreactive cells were significantly expressed more in the normal mucosa of cancer patients than in those with normal colonoscopy.<h4>Conclusion</h4>This is the first large series to demonstrate a composition change in the microbiota of colon cancer patients with possible impact on mucosal immune response. These data open new filed for mass screening and pathophysiology investigations.
format	article
author	Iradj Sobhani Julien Tap Françoise Roudot-Thoraval Jean P Roperch Sophie Letulle Philippe Langella Gérard Corthier Jeanne Tran Van Nhieu Jean P Furet
author_facet	Iradj Sobhani Julien Tap Françoise Roudot-Thoraval Jean P Roperch Sophie Letulle Philippe Langella Gérard Corthier Jeanne Tran Van Nhieu Jean P Furet
author_sort	Iradj Sobhani
title	Microbial dysbiosis in colorectal cancer (CRC) patients.
title_short	Microbial dysbiosis in colorectal cancer (CRC) patients.
title_full	Microbial dysbiosis in colorectal cancer (CRC) patients.
title_fullStr	Microbial dysbiosis in colorectal cancer (CRC) patients.
title_full_unstemmed	Microbial dysbiosis in colorectal cancer (CRC) patients.
title_sort	microbial dysbiosis in colorectal cancer (crc) patients.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/df92053d476b440095f4ffd4da31c1ca
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Microbial dysbiosis in colorectal cancer (CRC) patients.

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