Identification of novel genetic alterations in samples of malignant glioma patients.

Glioblastoma is the most frequent and malignant human brain tumor. High level of genomic instability detected in glioma cells implies that numerous genetic alterations accumulate during glioma pathogenesis. We investigated alterations in AP-PCR DNA profiles of 30 glioma patients, and detected specif...

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Autores principales:	Vedrana Milinkovic, Jasna Bankovic, Miodrag Rakic, Tijana Stankovic, Milica Skender-Gazibara, Sabera Ruzdijic, Nikola Tanic
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/df98bcafab9540dd9bc2ce3df71194b3
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spelling	oai:doaj.org-article:df98bcafab9540dd9bc2ce3df71194b32021-11-18T08:41:52ZIdentification of novel genetic alterations in samples of malignant glioma patients.1932-620310.1371/journal.pone.0082108https://doaj.org/article/df98bcafab9540dd9bc2ce3df71194b32013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24358143/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Glioblastoma is the most frequent and malignant human brain tumor. High level of genomic instability detected in glioma cells implies that numerous genetic alterations accumulate during glioma pathogenesis. We investigated alterations in AP-PCR DNA profiles of 30 glioma patients, and detected specific changes in 11 genes not previously associated with this disease: LHFPL3, SGCG, HTR4, ITGB1, CPS1, PROS1, GP2, KCNG2, PDE4D, KIR3DL3, and INPP5A. Further correlations revealed that 8 genes might play important role in pathogenesis of glial tumors, while changes in GP2, KCNG2 and KIR3DL3 should be considered as passenger mutations, consequence of high level of genomic instability. Identified genes have a significant role in signal transduction or cell adhesion, which are important processes for cancer development and progression. According to our results, LHFPL3 might be characteristic of primary glioblastoma, SGCG, HTR4, ITGB1, CPS1, PROS1 and INPP5A were detected predominantly in anaplastic astrocytoma, suggesting their role in progression of secondary glioblastoma, while alterations of PDE4D seem to have important role in development of both glioblastoma subtypes. Some of the identified genes showed significant association with p53, p16, and EGFR, but there was no significant correlation between loss of PTEN and any of identified genes. In conclusion our study revealed genetic alterations that were not previously associated with glioma pathogenesis and could be potentially used as molecular markers of different glioblastoma subtypes.Vedrana MilinkovicJasna BankovicMiodrag RakicTijana StankovicMilica Skender-GazibaraSabera RuzdijicNikola TanicPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e82108 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vedrana Milinkovic Jasna Bankovic Miodrag Rakic Tijana Stankovic Milica Skender-Gazibara Sabera Ruzdijic Nikola Tanic Identification of novel genetic alterations in samples of malignant glioma patients.
description	Glioblastoma is the most frequent and malignant human brain tumor. High level of genomic instability detected in glioma cells implies that numerous genetic alterations accumulate during glioma pathogenesis. We investigated alterations in AP-PCR DNA profiles of 30 glioma patients, and detected specific changes in 11 genes not previously associated with this disease: LHFPL3, SGCG, HTR4, ITGB1, CPS1, PROS1, GP2, KCNG2, PDE4D, KIR3DL3, and INPP5A. Further correlations revealed that 8 genes might play important role in pathogenesis of glial tumors, while changes in GP2, KCNG2 and KIR3DL3 should be considered as passenger mutations, consequence of high level of genomic instability. Identified genes have a significant role in signal transduction or cell adhesion, which are important processes for cancer development and progression. According to our results, LHFPL3 might be characteristic of primary glioblastoma, SGCG, HTR4, ITGB1, CPS1, PROS1 and INPP5A were detected predominantly in anaplastic astrocytoma, suggesting their role in progression of secondary glioblastoma, while alterations of PDE4D seem to have important role in development of both glioblastoma subtypes. Some of the identified genes showed significant association with p53, p16, and EGFR, but there was no significant correlation between loss of PTEN and any of identified genes. In conclusion our study revealed genetic alterations that were not previously associated with glioma pathogenesis and could be potentially used as molecular markers of different glioblastoma subtypes.
format	article
author	Vedrana Milinkovic Jasna Bankovic Miodrag Rakic Tijana Stankovic Milica Skender-Gazibara Sabera Ruzdijic Nikola Tanic
author_facet	Vedrana Milinkovic Jasna Bankovic Miodrag Rakic Tijana Stankovic Milica Skender-Gazibara Sabera Ruzdijic Nikola Tanic
author_sort	Vedrana Milinkovic
title	Identification of novel genetic alterations in samples of malignant glioma patients.
title_short	Identification of novel genetic alterations in samples of malignant glioma patients.
title_full	Identification of novel genetic alterations in samples of malignant glioma patients.
title_fullStr	Identification of novel genetic alterations in samples of malignant glioma patients.
title_full_unstemmed	Identification of novel genetic alterations in samples of malignant glioma patients.
title_sort	identification of novel genetic alterations in samples of malignant glioma patients.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/df98bcafab9540dd9bc2ce3df71194b3
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Identification of novel genetic alterations in samples of malignant glioma patients.

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