Evaluation Of Anti-Rabies Hyperimmune Serum Prepared Using Different Adjuvants

Anti-rabies virus hyperimmune serum was prepared in horses using both inactivated and live attenuated rabies virus (ERA strain) adjuvanted with three different adjuvants including 20% Alhydro gel, 5% Pet gel-A and 20% calcium phosphate gel. The six vaccine preparations were inoculated subcutaneousl...

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Autores principales:	Edries, S.M., Lamees, A, El – Tantawy, Wafaa, R, Abdel Aziz, Ibrahim, H.M., Omaima A.A. Al-Shamandy
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2020
Materias:	elisa era hyperimmune serum rabies serum neutralization Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2020.21969.1003 https://doaj.org/article/df9af96dca554be2868364e48ede73b8
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spelling	oai:doaj.org-article:df9af96dca554be2868364e48ede73b82021-12-02T11:43:50ZEvaluation Of Anti-Rabies Hyperimmune Serum Prepared Using Different Adjuvantshttps://dx.doi.org/10.21608/javs.2020.21969.10031687-40722090-3308https://doaj.org/article/df9af96dca554be2868364e48ede73b82020-01-01T00:00:00Zhttps://javs.journals.ekb.eg/article_69874.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308Anti-rabies virus hyperimmune serum was prepared in horses using both inactivated and live attenuated rabies virus (ERA strain) adjuvanted with three different adjuvants including 20% Alhydro gel, 5% Pet gel-A and 20% calcium phosphate gel. The six vaccine preparations were inoculated subcutaneously in three groups of horses, separately, as each horse group received 4 increased doses of an inactivated vaccine followed by 4 increased doses of a live attenuated vaccine (one dose twice weekly) with the same adjuvant. All prepared vaccine formulae were found to be free from foreign contaminants and safe with no post inoculation abnormal signs in inoculated mice. Monitoring of the levels of exhibited rabies antibodies in the sera of immunized horses using serum neutralization test (SNT) and quantitative ELISA kit revealed that the prepared rabies vaccines with 5% Pet gel A adjuvant induced the highest antibody titers (2048 by SNT and 2.20 by ELISA kit) with expected protection percentage 96.14% followed by those induced by 20% Calcium phosphate gel recording the values of 2048 by SNT and 2.11 by ELISA kit with expected protection percentage 92.2%. Lower values (1024 by SNT; 2.05 by ELISA kit and 89.59% protection percentage) were recorded for the Alhydro gel adjuvanted vaccine. These obtained data reflected the potency of the prepared equine anti-rabies hyperimmune serum (EARHIS) to be used for post exposure treatment in emergency cases and that is the Pet gel A was the best adjuvant to be used.Edries, S.M.Lamees, A, El – TantawyWafaa, R, Abdel AzizIbrahim, H.M.Omaima A.A. Al-ShamandyEgyptian Society for Animal Managementarticleelisaerahyperimmune serumrabiesserum neutralizationZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 5, Iss 1, Pp 17-21 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	elisa era hyperimmune serum rabies serum neutralization Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	elisa era hyperimmune serum rabies serum neutralization Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Edries, S.M. Lamees, A, El – Tantawy Wafaa, R, Abdel Aziz Ibrahim, H.M. Omaima A.A. Al-Shamandy Evaluation Of Anti-Rabies Hyperimmune Serum Prepared Using Different Adjuvants
description	Anti-rabies virus hyperimmune serum was prepared in horses using both inactivated and live attenuated rabies virus (ERA strain) adjuvanted with three different adjuvants including 20% Alhydro gel, 5% Pet gel-A and 20% calcium phosphate gel. The six vaccine preparations were inoculated subcutaneously in three groups of horses, separately, as each horse group received 4 increased doses of an inactivated vaccine followed by 4 increased doses of a live attenuated vaccine (one dose twice weekly) with the same adjuvant. All prepared vaccine formulae were found to be free from foreign contaminants and safe with no post inoculation abnormal signs in inoculated mice. Monitoring of the levels of exhibited rabies antibodies in the sera of immunized horses using serum neutralization test (SNT) and quantitative ELISA kit revealed that the prepared rabies vaccines with 5% Pet gel A adjuvant induced the highest antibody titers (2048 by SNT and 2.20 by ELISA kit) with expected protection percentage 96.14% followed by those induced by 20% Calcium phosphate gel recording the values of 2048 by SNT and 2.11 by ELISA kit with expected protection percentage 92.2%. Lower values (1024 by SNT; 2.05 by ELISA kit and 89.59% protection percentage) were recorded for the Alhydro gel adjuvanted vaccine. These obtained data reflected the potency of the prepared equine anti-rabies hyperimmune serum (EARHIS) to be used for post exposure treatment in emergency cases and that is the Pet gel A was the best adjuvant to be used.
format	article
author	Edries, S.M. Lamees, A, El – Tantawy Wafaa, R, Abdel Aziz Ibrahim, H.M. Omaima A.A. Al-Shamandy
author_facet	Edries, S.M. Lamees, A, El – Tantawy Wafaa, R, Abdel Aziz Ibrahim, H.M. Omaima A.A. Al-Shamandy
author_sort	Edries, S.M.
title	Evaluation Of Anti-Rabies Hyperimmune Serum Prepared Using Different Adjuvants
title_short	Evaluation Of Anti-Rabies Hyperimmune Serum Prepared Using Different Adjuvants
title_full	Evaluation Of Anti-Rabies Hyperimmune Serum Prepared Using Different Adjuvants
title_fullStr	Evaluation Of Anti-Rabies Hyperimmune Serum Prepared Using Different Adjuvants
title_full_unstemmed	Evaluation Of Anti-Rabies Hyperimmune Serum Prepared Using Different Adjuvants
title_sort	evaluation of anti-rabies hyperimmune serum prepared using different adjuvants
publisher	Egyptian Society for Animal Management
publishDate	2020
url	https://dx.doi.org/10.21608/javs.2020.21969.1003 https://doaj.org/article/df9af96dca554be2868364e48ede73b8
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Evaluation Of Anti-Rabies Hyperimmune Serum Prepared Using Different Adjuvants

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