<i>Fusarium Oxysporum</i> f. sp. <i>Cannabis</i> Isolated from <i>Cannabis Sativa</i> L.: In Vitro and In Planta Biocontrol by a Plant Growth Promoting-Bacteria Consortium
Industrial hemp (<i>Cannabis sativa</i> L.) is a multipurpose plant used in several fields. Several phytopathogens attack hemp crops. <i>Fusarium oxysporum</i> is a common fungal pathogen that causes wilt disease in nurseries and in field cultivation and causes high losses. I...
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| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/df9dc28375b647859fba131cd630d04a |
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| Sumario: | Industrial hemp (<i>Cannabis sativa</i> L.) is a multipurpose plant used in several fields. Several phytopathogens attack hemp crops. <i>Fusarium oxysporum</i> is a common fungal pathogen that causes wilt disease in nurseries and in field cultivation and causes high losses. In the present study, a pathogenic strain belonging to <i>F. oxysporum</i> f. sp. <i>cannabis</i> was isolated from a plant showing <i>Fusarium</i> wilt. After isolation, identification was conducted based on morphological and molecular characterizations and pathogenicity tests. Selected plant growth-promoting bacteria with interesting biocontrol properties—<i>Azospirillum brasilense</i>, <i>Gluconacetobacter diazotrophicus</i>, <i>Herbaspirillum seropedicae</i> and <i>Burkholderia ambifaria</i>—were tested against this pathogen. In vitro antagonistic activity was determined by the dual culture method. Effective strains (in vitro inhibition > of 50%) <i>G. diazotrophicus</i>, <i>H. seropedicae</i> and <i>B. ambifaria</i> were combined in a consortium and screened for in planta antagonistic activity in pre-emergence (before germination) and post-emergence (after germination). The consortium counteracted <i>Fusarium</i> infection both in pre-emergence and post-emergence. Our preliminary results show that the selected consortium could be further investigated as an effective biocontrol agent for the management of this pathogen. |
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