<i>Fusarium Oxysporum</i> f. sp. <i>Cannabis</i> Isolated from <i>Cannabis Sativa</i> L.: In Vitro and In Planta Biocontrol by a Plant Growth Promoting-Bacteria Consortium

<i>Fusarium Oxysporum</i> f. sp. <i>Cannabis</i> Isolated from <i>Cannabis Sativa</i> L.: In Vitro and In Planta Biocontrol by a Plant Growth Promoting-Bacteria Consortium

Industrial hemp (<i>Cannabis sativa</i> L.) is a multipurpose plant used in several fields. Several phytopathogens attack hemp crops. <i>Fusarium oxysporum</i> is a common fungal pathogen that causes wilt disease in nurseries and in field cultivation and causes high losses. I...

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Autores principales: Marika Pellegrini, Claudia Ercole, Carmelo Gianchino, Matteo Bernardi, Loretta Pace, Maddalena Del Gallo
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
PGPB
biocontrol
<i>Fusarium</i> wilt
industrial hemp
ITS barcoding
scanning electron microscopy
Botany
QK1-989
Acceso en línea:https://doaj.org/article/df9dc28375b647859fba131cd630d04a
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spelling oai:doaj.org-article:df9dc28375b647859fba131cd630d04a2021-11-25T18:46:35Z<i>Fusarium Oxysporum</i> f. sp. <i>Cannabis</i> Isolated from <i>Cannabis Sativa</i> L.: In Vitro and In Planta Biocontrol by a Plant Growth Promoting-Bacteria Consortium10.3390/plants101124362223-7747https://doaj.org/article/df9dc28375b647859fba131cd630d04a2021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2436https://doaj.org/toc/2223-7747Industrial hemp (<i>Cannabis sativa</i> L.) is a multipurpose plant used in several fields. Several phytopathogens attack hemp crops. <i>Fusarium oxysporum</i> is a common fungal pathogen that causes wilt disease in nurseries and in field cultivation and causes high losses. In the present study, a pathogenic strain belonging to <i>F. oxysporum</i> f. sp. <i>cannabis</i> was isolated from a plant showing <i>Fusarium</i> wilt. After isolation, identification was conducted based on morphological and molecular characterizations and pathogenicity tests. Selected plant growth-promoting bacteria with interesting biocontrol properties—<i>Azospirillum brasilense</i>, <i>Gluconacetobacter diazotrophicus</i>, <i>Herbaspirillum seropedicae</i> and <i>Burkholderia ambifaria</i>—were tested against this pathogen. In vitro antagonistic activity was determined by the dual culture method. Effective strains (in vitro inhibition > of 50%) <i>G. diazotrophicus</i>, <i>H. seropedicae</i> and <i>B. ambifaria</i> were combined in a consortium and screened for in planta antagonistic activity in pre-emergence (before germination) and post-emergence (after germination). The consortium counteracted <i>Fusarium</i> infection both in pre-emergence and post-emergence. Our preliminary results show that the selected consortium could be further investigated as an effective biocontrol agent for the management of this pathogen.Marika PellegriniClaudia ErcoleCarmelo GianchinoMatteo BernardiLoretta PaceMaddalena Del GalloMDPI AGarticlePGPBbiocontrol<i>Fusarium</i> wiltindustrial hempITS barcodingscanning electron microscopyBotanyQK1-989ENPlants, Vol 10, Iss 2436, p 2436 (2021)
institution DOAJ
collection DOAJ
language EN
topic PGPB
biocontrol
<i>Fusarium</i> wilt
industrial hemp
ITS barcoding
scanning electron microscopy
Botany
QK1-989
spellingShingle PGPB
biocontrol
<i>Fusarium</i> wilt
industrial hemp
ITS barcoding
scanning electron microscopy
Botany
QK1-989
Marika Pellegrini
Claudia Ercole
Carmelo Gianchino
Matteo Bernardi
Loretta Pace
Maddalena Del Gallo
<i>Fusarium Oxysporum</i> f. sp. <i>Cannabis</i> Isolated from <i>Cannabis Sativa</i> L.: In Vitro and In Planta Biocontrol by a Plant Growth Promoting-Bacteria Consortium
description Industrial hemp (<i>Cannabis sativa</i> L.) is a multipurpose plant used in several fields. Several phytopathogens attack hemp crops. <i>Fusarium oxysporum</i> is a common fungal pathogen that causes wilt disease in nurseries and in field cultivation and causes high losses. In the present study, a pathogenic strain belonging to <i>F. oxysporum</i> f. sp. <i>cannabis</i> was isolated from a plant showing <i>Fusarium</i> wilt. After isolation, identification was conducted based on morphological and molecular characterizations and pathogenicity tests. Selected plant growth-promoting bacteria with interesting biocontrol properties—<i>Azospirillum brasilense</i>, <i>Gluconacetobacter diazotrophicus</i>, <i>Herbaspirillum seropedicae</i> and <i>Burkholderia ambifaria</i>—were tested against this pathogen. In vitro antagonistic activity was determined by the dual culture method. Effective strains (in vitro inhibition > of 50%) <i>G. diazotrophicus</i>, <i>H. seropedicae</i> and <i>B. ambifaria</i> were combined in a consortium and screened for in planta antagonistic activity in pre-emergence (before germination) and post-emergence (after germination). The consortium counteracted <i>Fusarium</i> infection both in pre-emergence and post-emergence. Our preliminary results show that the selected consortium could be further investigated as an effective biocontrol agent for the management of this pathogen.
format article
author Marika Pellegrini
Claudia Ercole
Carmelo Gianchino
Matteo Bernardi
Loretta Pace
Maddalena Del Gallo
author_facet Marika Pellegrini
Claudia Ercole
Carmelo Gianchino
Matteo Bernardi
Loretta Pace
Maddalena Del Gallo
author_sort Marika Pellegrini
title <i>Fusarium Oxysporum</i> f. sp. <i>Cannabis</i> Isolated from <i>Cannabis Sativa</i> L.: In Vitro and In Planta Biocontrol by a Plant Growth Promoting-Bacteria Consortium
title_short <i>Fusarium Oxysporum</i> f. sp. <i>Cannabis</i> Isolated from <i>Cannabis Sativa</i> L.: In Vitro and In Planta Biocontrol by a Plant Growth Promoting-Bacteria Consortium
title_full <i>Fusarium Oxysporum</i> f. sp. <i>Cannabis</i> Isolated from <i>Cannabis Sativa</i> L.: In Vitro and In Planta Biocontrol by a Plant Growth Promoting-Bacteria Consortium
title_fullStr <i>Fusarium Oxysporum</i> f. sp. <i>Cannabis</i> Isolated from <i>Cannabis Sativa</i> L.: In Vitro and In Planta Biocontrol by a Plant Growth Promoting-Bacteria Consortium
title_full_unstemmed <i>Fusarium Oxysporum</i> f. sp. <i>Cannabis</i> Isolated from <i>Cannabis Sativa</i> L.: In Vitro and In Planta Biocontrol by a Plant Growth Promoting-Bacteria Consortium
title_sort <i>fusarium oxysporum</i> f. sp. <i>cannabis</i> isolated from <i>cannabis sativa</i> l.: in vitro and in planta biocontrol by a plant growth promoting-bacteria consortium
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/df9dc28375b647859fba131cd630d04a
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