In vivo imaging of fluorescent single-walled carbon nanotubes within C. elegans nematodes in the near-infrared window

Caenorhabditis elegans (C. elegans) nematodes serve as a model organism for eukaryotes, especially due to their genetic similarity. Although they have many advantages like their small size and transparency, their autofluorescence in the entire visible wavelength range poses a challenge for imaging a...

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Autores principales:	Adi Hendler-Neumark, Verena Wulf, Gili Bisker
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Caenorhabditis elegans Autofluorescence Single-walled carbon nanotubes Near-infrared fluorescent nanoparticles In vivo imaging Medicine (General) R5-920 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/dfa09104694e440c8a92418b8a49c48c
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id	oai:doaj.org-article:dfa09104694e440c8a92418b8a49c48c
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spelling	oai:doaj.org-article:dfa09104694e440c8a92418b8a49c48c2021-12-04T04:35:38ZIn vivo imaging of fluorescent single-walled carbon nanotubes within C. elegans nematodes in the near-infrared window2590-006410.1016/j.mtbio.2021.100175https://doaj.org/article/dfa09104694e440c8a92418b8a49c48c2021-09-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2590006421000831https://doaj.org/toc/2590-0064Caenorhabditis elegans (C. elegans) nematodes serve as a model organism for eukaryotes, especially due to their genetic similarity. Although they have many advantages like their small size and transparency, their autofluorescence in the entire visible wavelength range poses a challenge for imaging and tracking fluorescent proteins or dyes using standard fluorescence microscopy. Herein, near-infrared (NIR) fluorescent single-walled carbon nanotubes (SWCNTs) are utilized for in vivo imaging within the gastrointestinal track of C. elegans. The SWCNTs are biocompatible, and do not affect the worms’ viability nor their reproduction ability. The worms do not show any autofluorescence in the NIR range, thus enabling the spectral separation between the SWCNT NIR fluorescence and the strong autofluorescence of the worm gut granules. The worms are fed with ssDNA-SWCNT which are visualized mainly in the intestine lumen. The NIR fluorescence is used in vivo to track the contraction and relaxation in the area of the pharyngeal valve at the anterior of the terminal bulb. These biocompatible, non-photobleaching, NIR fluorescent nanoparticles can advance in vivo imaging and tracking within C. elegans and other small model organisms by overcoming the signal-to-noise challenge stemming from the wide-range visible autofluorescence.Adi Hendler-NeumarkVerena WulfGili BiskerElsevierarticleCaenorhabditis elegansAutofluorescenceSingle-walled carbon nanotubesNear-infrared fluorescent nanoparticlesIn vivo imagingMedicine (General)R5-920Biology (General)QH301-705.5ENMaterials Today Bio, Vol 12, Iss , Pp 100175- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Caenorhabditis elegans Autofluorescence Single-walled carbon nanotubes Near-infrared fluorescent nanoparticles In vivo imaging Medicine (General) R5-920 Biology (General) QH301-705.5
spellingShingle	Caenorhabditis elegans Autofluorescence Single-walled carbon nanotubes Near-infrared fluorescent nanoparticles In vivo imaging Medicine (General) R5-920 Biology (General) QH301-705.5 Adi Hendler-Neumark Verena Wulf Gili Bisker In vivo imaging of fluorescent single-walled carbon nanotubes within C. elegans nematodes in the near-infrared window
description	Caenorhabditis elegans (C. elegans) nematodes serve as a model organism for eukaryotes, especially due to their genetic similarity. Although they have many advantages like their small size and transparency, their autofluorescence in the entire visible wavelength range poses a challenge for imaging and tracking fluorescent proteins or dyes using standard fluorescence microscopy. Herein, near-infrared (NIR) fluorescent single-walled carbon nanotubes (SWCNTs) are utilized for in vivo imaging within the gastrointestinal track of C. elegans. The SWCNTs are biocompatible, and do not affect the worms’ viability nor their reproduction ability. The worms do not show any autofluorescence in the NIR range, thus enabling the spectral separation between the SWCNT NIR fluorescence and the strong autofluorescence of the worm gut granules. The worms are fed with ssDNA-SWCNT which are visualized mainly in the intestine lumen. The NIR fluorescence is used in vivo to track the contraction and relaxation in the area of the pharyngeal valve at the anterior of the terminal bulb. These biocompatible, non-photobleaching, NIR fluorescent nanoparticles can advance in vivo imaging and tracking within C. elegans and other small model organisms by overcoming the signal-to-noise challenge stemming from the wide-range visible autofluorescence.
format	article
author	Adi Hendler-Neumark Verena Wulf Gili Bisker
author_facet	Adi Hendler-Neumark Verena Wulf Gili Bisker
author_sort	Adi Hendler-Neumark
title	In vivo imaging of fluorescent single-walled carbon nanotubes within C. elegans nematodes in the near-infrared window
title_short	In vivo imaging of fluorescent single-walled carbon nanotubes within C. elegans nematodes in the near-infrared window
title_full	In vivo imaging of fluorescent single-walled carbon nanotubes within C. elegans nematodes in the near-infrared window
title_fullStr	In vivo imaging of fluorescent single-walled carbon nanotubes within C. elegans nematodes in the near-infrared window
title_full_unstemmed	In vivo imaging of fluorescent single-walled carbon nanotubes within C. elegans nematodes in the near-infrared window
title_sort	in vivo imaging of fluorescent single-walled carbon nanotubes within c. elegans nematodes in the near-infrared window
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/dfa09104694e440c8a92418b8a49c48c
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In vivo imaging of fluorescent single-walled carbon nanotubes within C. elegans nematodes in the near-infrared window

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