IncA/C Conjugative Plasmids Mobilize a New Family of Multidrug Resistance Islands in Clinical <named-content content-type="genus-species">Vibrio cholerae</named-content> Non-O1/Non-O139 Isolates from Haiti

ABSTRACT Mobile genetic elements play a pivotal role in the adaptation of bacterial populations, allowing them to rapidly cope with hostile conditions, including the presence of antimicrobial compounds. IncA/C conjugative plasmids (ACPs) are efficient vehicles for dissemination of multidrug resistan...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nicolas Carraro, Nicolas Rivard, Daniela Ceccarelli, Rita R. Colwell, Vincent Burrus
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/dfa1cd5071a54bffbc9abde942473c66
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:dfa1cd5071a54bffbc9abde942473c66
record_format	dspace
spelling	oai:doaj.org-article:dfa1cd5071a54bffbc9abde942473c662021-11-15T15:50:18ZIncA/C Conjugative Plasmids Mobilize a New Family of Multidrug Resistance Islands in Clinical <named-content content-type="genus-species">Vibrio cholerae</named-content> Non-O1/Non-O139 Isolates from Haiti10.1128/mBio.00509-162150-7511https://doaj.org/article/dfa1cd5071a54bffbc9abde942473c662016-09-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00509-16https://doaj.org/toc/2150-7511ABSTRACT Mobile genetic elements play a pivotal role in the adaptation of bacterial populations, allowing them to rapidly cope with hostile conditions, including the presence of antimicrobial compounds. IncA/C conjugative plasmids (ACPs) are efficient vehicles for dissemination of multidrug resistance genes in a broad range of pathogenic species of Enterobacteriaceae. ACPs have sporadically been reported in Vibrio cholerae, the infectious agent of the diarrheal disease cholera. The regulatory network that controls ACP mobility ultimately depends on the transcriptional activation of multiple ACP-borne operons by the master activator AcaCD. Beyond ACP conjugation, AcaCD has also recently been shown to activate the expression of genes located in the Salmonella genomic island 1 (SGI1). Here, we describe MGIVchHai6, a novel and unrelated mobilizable genomic island (MGI) integrated into the 3′ end of trmE in chromosome I of V. cholerae HC-36A1, a non-O1/non-O139 multidrug-resistant clinical isolate recovered from Haiti in 2010. MGIVchHai6 contains a mercury resistance transposon and an integron In104-like multidrug resistance element similar to the one of SGI1. We show that MGIVchHai6 excises from the chromosome in an AcaCD-dependent manner and is mobilized by ACPs. Acquisition of MGIVchHai6 confers resistance to β-lactams, sulfamethoxazole, tetracycline, chloramphenicol, trimethoprim, and streptomycin/spectinomycin. In silico analyses revealed that MGIVchHai6-like elements are carried by several environmental and clinical V. cholerae strains recovered from the Indian subcontinent, as well as from North and South America, including all non-O1/non-O139 clinical isolates from Haiti. IMPORTANCE Vibrio cholerae, the causative agent of cholera, remains a global public health threat. Seventh-pandemic V. cholerae acquired multidrug resistance genes primarily through circulation of SXT/R391 integrative and conjugative elements. IncA/C conjugative plasmids have sporadically been reported to mediate antimicrobial resistance in environmental and clinical V. cholerae isolates. Our results showed that while IncA/C plasmids are rare in V. cholerae populations, they play an important yet insidious role by specifically propagating a new family of genomic islands conferring resistance to multiple antibiotics. These results suggest that nonepidemic V. cholerae non-O1/non-O139 strains bearing these genomic islands constitute a reservoir of transmissible resistance genes that can be propagated by IncA/C plasmids to V. cholerae populations in epidemic geographical areas as well to pathogenic species of Enterobacteriaceae. We recommend future epidemiological surveys take into account the circulation of these genomic islands.Nicolas CarraroNicolas RivardDaniela CeccarelliRita R. ColwellVincent BurrusAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 7, Iss 4 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Nicolas Carraro Nicolas Rivard Daniela Ceccarelli Rita R. Colwell Vincent Burrus IncA/C Conjugative Plasmids Mobilize a New Family of Multidrug Resistance Islands in Clinical <named-content content-type="genus-species">Vibrio cholerae</named-content> Non-O1/Non-O139 Isolates from Haiti
description	ABSTRACT Mobile genetic elements play a pivotal role in the adaptation of bacterial populations, allowing them to rapidly cope with hostile conditions, including the presence of antimicrobial compounds. IncA/C conjugative plasmids (ACPs) are efficient vehicles for dissemination of multidrug resistance genes in a broad range of pathogenic species of Enterobacteriaceae. ACPs have sporadically been reported in Vibrio cholerae, the infectious agent of the diarrheal disease cholera. The regulatory network that controls ACP mobility ultimately depends on the transcriptional activation of multiple ACP-borne operons by the master activator AcaCD. Beyond ACP conjugation, AcaCD has also recently been shown to activate the expression of genes located in the Salmonella genomic island 1 (SGI1). Here, we describe MGIVchHai6, a novel and unrelated mobilizable genomic island (MGI) integrated into the 3′ end of trmE in chromosome I of V. cholerae HC-36A1, a non-O1/non-O139 multidrug-resistant clinical isolate recovered from Haiti in 2010. MGIVchHai6 contains a mercury resistance transposon and an integron In104-like multidrug resistance element similar to the one of SGI1. We show that MGIVchHai6 excises from the chromosome in an AcaCD-dependent manner and is mobilized by ACPs. Acquisition of MGIVchHai6 confers resistance to β-lactams, sulfamethoxazole, tetracycline, chloramphenicol, trimethoprim, and streptomycin/spectinomycin. In silico analyses revealed that MGIVchHai6-like elements are carried by several environmental and clinical V. cholerae strains recovered from the Indian subcontinent, as well as from North and South America, including all non-O1/non-O139 clinical isolates from Haiti. IMPORTANCE Vibrio cholerae, the causative agent of cholera, remains a global public health threat. Seventh-pandemic V. cholerae acquired multidrug resistance genes primarily through circulation of SXT/R391 integrative and conjugative elements. IncA/C conjugative plasmids have sporadically been reported to mediate antimicrobial resistance in environmental and clinical V. cholerae isolates. Our results showed that while IncA/C plasmids are rare in V. cholerae populations, they play an important yet insidious role by specifically propagating a new family of genomic islands conferring resistance to multiple antibiotics. These results suggest that nonepidemic V. cholerae non-O1/non-O139 strains bearing these genomic islands constitute a reservoir of transmissible resistance genes that can be propagated by IncA/C plasmids to V. cholerae populations in epidemic geographical areas as well to pathogenic species of Enterobacteriaceae. We recommend future epidemiological surveys take into account the circulation of these genomic islands.
format	article
author	Nicolas Carraro Nicolas Rivard Daniela Ceccarelli Rita R. Colwell Vincent Burrus
author_facet	Nicolas Carraro Nicolas Rivard Daniela Ceccarelli Rita R. Colwell Vincent Burrus
author_sort	Nicolas Carraro
title	IncA/C Conjugative Plasmids Mobilize a New Family of Multidrug Resistance Islands in Clinical <named-content content-type="genus-species">Vibrio cholerae</named-content> Non-O1/Non-O139 Isolates from Haiti
title_short	IncA/C Conjugative Plasmids Mobilize a New Family of Multidrug Resistance Islands in Clinical <named-content content-type="genus-species">Vibrio cholerae</named-content> Non-O1/Non-O139 Isolates from Haiti
title_full	IncA/C Conjugative Plasmids Mobilize a New Family of Multidrug Resistance Islands in Clinical <named-content content-type="genus-species">Vibrio cholerae</named-content> Non-O1/Non-O139 Isolates from Haiti
title_fullStr	IncA/C Conjugative Plasmids Mobilize a New Family of Multidrug Resistance Islands in Clinical <named-content content-type="genus-species">Vibrio cholerae</named-content> Non-O1/Non-O139 Isolates from Haiti
title_full_unstemmed	IncA/C Conjugative Plasmids Mobilize a New Family of Multidrug Resistance Islands in Clinical <named-content content-type="genus-species">Vibrio cholerae</named-content> Non-O1/Non-O139 Isolates from Haiti
title_sort	inca/c conjugative plasmids mobilize a new family of multidrug resistance islands in clinical <named-content content-type="genus-species">vibrio cholerae</named-content> non-o1/non-o139 isolates from haiti
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/dfa1cd5071a54bffbc9abde942473c66
work_keys_str_mv	AT nicolascarraro incacconjugativeplasmidsmobilizeanewfamilyofmultidrugresistanceislandsinclinicalnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontentnono1nono139isolatesfromhaiti AT nicolasrivard incacconjugativeplasmidsmobilizeanewfamilyofmultidrugresistanceislandsinclinicalnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontentnono1nono139isolatesfromhaiti AT danielaceccarelli incacconjugativeplasmidsmobilizeanewfamilyofmultidrugresistanceislandsinclinicalnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontentnono1nono139isolatesfromhaiti AT ritarcolwell incacconjugativeplasmidsmobilizeanewfamilyofmultidrugresistanceislandsinclinicalnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontentnono1nono139isolatesfromhaiti AT vincentburrus incacconjugativeplasmidsmobilizeanewfamilyofmultidrugresistanceislandsinclinicalnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontentnono1nono139isolatesfromhaiti
_version_	1718427430478151680

IncA/C Conjugative Plasmids Mobilize a New Family of Multidrug Resistance Islands in Clinical <named-content content-type="genus-species">Vibrio cholerae</named-content> Non-O1/Non-O139 Isolates from Haiti

Ejemplares similares