Changes in culture expanded human amniotic epithelial cells: implications for potential therapeutic applications.

Human amniotic epithelial cells (hAEC) isolated from term placenta have stem cell-like properties, differentiate into tissue specific cells and reduce lung and liver inflammation and fibrosis following transplantation into disease models established in mice. These features together with their low im...

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Autores principales:	Gita Pratama, Vijesh Vaghjiani, Jing Yang Tee, Yu Han Liu, James Chan, Charmaine Tan, Padma Murthi, Caroline Gargett, Ursula Manuelpillai
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dfa4d0631dd9448a8aa0be3f063f1e29
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spelling	oai:doaj.org-article:dfa4d0631dd9448a8aa0be3f063f1e292021-11-18T07:35:13ZChanges in culture expanded human amniotic epithelial cells: implications for potential therapeutic applications.1932-620310.1371/journal.pone.0026136https://doaj.org/article/dfa4d0631dd9448a8aa0be3f063f1e292011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22073147/?tool=EBIhttps://doaj.org/toc/1932-6203Human amniotic epithelial cells (hAEC) isolated from term placenta have stem cell-like properties, differentiate into tissue specific cells and reduce lung and liver inflammation and fibrosis following transplantation into disease models established in mice. These features together with their low immunogenicity and immunosuppressive properties make hAEC an attractive source of cells for potential therapeutic applications. However, generation of large cell numbers required for therapies through serial expansion in xenobiotic-free media may be a limiting factor. We investigated if hAEC could be expanded in xenobiotic-free media and if expansion altered their differentiation capacity, immunophenotype, immunosuppressive properties and production of immunomodulatory factors. Serial expansion in xenobiotic-free media was limited with cumulative cell numbers and population doubling times significantly lower than controls maintained in fetal calf serum. The epithelial morphology of primary hAEC changed into mesenchymal-stromal like cells by passage 4-5 (P4-P5) with down regulation of epithelial markers CK7, CD49f, EpCAM and E-cadherin and elevation of mesenchymal-stromal markers CD44, CD105, CD146 and vimentin. The P5 hAEC expanded in xenobiotic-free medium differentiated into osteocyte and alveolar epithelium-like cells, but not chondrocyte, hepatocyte, α- and β-pancreatic-like cells. Expression of HLA Class IA, Class II and co-stimulatory molecules CD80, CD86 and CD40 remained unaltered. The P5 hAEC suppressed mitogen stimulated T cell proliferation, but were less suppressive compared with primary hAEC at higher splenocyte ratios. Primary and P5 hAEC did not secrete the immunosuppressive factors IL-10 and HGF, whereas TGF-β1 and HLA-G were reduced and IL-6 elevated in P5 hAEC. These findings suggest that primary and expanded hAEC may be suitable for different cellular therapeutic applications.Gita PratamaVijesh VaghjianiJing Yang TeeYu Han LiuJames ChanCharmaine TanPadma MurthiCaroline GargettUrsula ManuelpillaiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 11, p e26136 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gita Pratama Vijesh Vaghjiani Jing Yang Tee Yu Han Liu James Chan Charmaine Tan Padma Murthi Caroline Gargett Ursula Manuelpillai Changes in culture expanded human amniotic epithelial cells: implications for potential therapeutic applications.
description	Human amniotic epithelial cells (hAEC) isolated from term placenta have stem cell-like properties, differentiate into tissue specific cells and reduce lung and liver inflammation and fibrosis following transplantation into disease models established in mice. These features together with their low immunogenicity and immunosuppressive properties make hAEC an attractive source of cells for potential therapeutic applications. However, generation of large cell numbers required for therapies through serial expansion in xenobiotic-free media may be a limiting factor. We investigated if hAEC could be expanded in xenobiotic-free media and if expansion altered their differentiation capacity, immunophenotype, immunosuppressive properties and production of immunomodulatory factors. Serial expansion in xenobiotic-free media was limited with cumulative cell numbers and population doubling times significantly lower than controls maintained in fetal calf serum. The epithelial morphology of primary hAEC changed into mesenchymal-stromal like cells by passage 4-5 (P4-P5) with down regulation of epithelial markers CK7, CD49f, EpCAM and E-cadherin and elevation of mesenchymal-stromal markers CD44, CD105, CD146 and vimentin. The P5 hAEC expanded in xenobiotic-free medium differentiated into osteocyte and alveolar epithelium-like cells, but not chondrocyte, hepatocyte, α- and β-pancreatic-like cells. Expression of HLA Class IA, Class II and co-stimulatory molecules CD80, CD86 and CD40 remained unaltered. The P5 hAEC suppressed mitogen stimulated T cell proliferation, but were less suppressive compared with primary hAEC at higher splenocyte ratios. Primary and P5 hAEC did not secrete the immunosuppressive factors IL-10 and HGF, whereas TGF-β1 and HLA-G were reduced and IL-6 elevated in P5 hAEC. These findings suggest that primary and expanded hAEC may be suitable for different cellular therapeutic applications.
format	article
author	Gita Pratama Vijesh Vaghjiani Jing Yang Tee Yu Han Liu James Chan Charmaine Tan Padma Murthi Caroline Gargett Ursula Manuelpillai
author_facet	Gita Pratama Vijesh Vaghjiani Jing Yang Tee Yu Han Liu James Chan Charmaine Tan Padma Murthi Caroline Gargett Ursula Manuelpillai
author_sort	Gita Pratama
title	Changes in culture expanded human amniotic epithelial cells: implications for potential therapeutic applications.
title_short	Changes in culture expanded human amniotic epithelial cells: implications for potential therapeutic applications.
title_full	Changes in culture expanded human amniotic epithelial cells: implications for potential therapeutic applications.
title_fullStr	Changes in culture expanded human amniotic epithelial cells: implications for potential therapeutic applications.
title_full_unstemmed	Changes in culture expanded human amniotic epithelial cells: implications for potential therapeutic applications.
title_sort	changes in culture expanded human amniotic epithelial cells: implications for potential therapeutic applications.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/dfa4d0631dd9448a8aa0be3f063f1e29
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Changes in culture expanded human amniotic epithelial cells: implications for potential therapeutic applications.

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