Sex differences of inflammation in target organs, induced intraperitoneal injection of lipopolysaccharide, depend on its dose

Anna M Kosyreva,1 Olga V Makarova,1 Lev V Kakturskiy,2 Liliya P Mikhailova,1 Marina N Boltovskaya,3 Konstantin A Rogov2 1Department of Immunomorphology of Inflammation, Federal State Budgetary Institution “Science Research Institute of Human Morphology”, Moscow, Russia; 2Departme...

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Autores principales:	Kosyreva AM, Makarova OV, Kakturskiy LV, Mikhailova LP, Boltovskaya MN, Rogov KA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	sex differences inflammation SIRS endotoxin high- and low-dose LPS Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/dfb2921088b74bebb99bab7e9e6fa31c
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spelling	oai:doaj.org-article:dfb2921088b74bebb99bab7e9e6fa31c2021-12-02T02:59:20ZSex differences of inflammation in target organs, induced intraperitoneal injection of lipopolysaccharide, depend on its dose1178-7031https://doaj.org/article/dfb2921088b74bebb99bab7e9e6fa31c2018-11-01T00:00:00Zhttps://www.dovepress.com/sex-differences-of-inflammation-in-target-organs-induced-intraperitone-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Anna M Kosyreva,1 Olga V Makarova,1 Lev V Kakturskiy,2 Liliya P Mikhailova,1 Marina N Boltovskaya,3 Konstantin A Rogov2 1Department of Immunomorphology of Inflammation, Federal State Budgetary Institution “Science Research Institute of Human Morphology”, Moscow, Russia; 2Department of Pathology, Federal State Budgetary Institution “Science Research Institute of Human Morphology”, Moscow, Russia; 3Department of Reproductive Pathology, Federal State Budgetary Institution “Science Research Institute of Human Morphology”, Moscow, Russia Purpose: The aim of our research was to study sex differences and the severity of inflammatory changes in target organs and the peculiarities of immunological disorders when low and high doses of lipopolysaccharide (LPS) were administered to rats. Methods: Male and female 2- to 3-month-old Wistar rats (200–250 g) were injected intraperitoneally with Escherichia coli LPS in one of two doses: 1.5 or 15 mg/kg. In a day after the LPS injection, we studied endotoxin, corticosterone, sex steroids, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) activity levels in the serum; morphological disorders in the lung, liver, thymus, and spleen; ex vivo production of IL-2, IL-4, tumor necrosis factor (TNF), and interferon γ (IFNγ) by splenic cells activated by ConA; and relative amount of T- and B-lymphocytes in the peripheral blood. Results: After the injection of low-dose LPS, the serum endotoxin level increased only in males and was combined with a more pronounced inflammatory response in the lungs and thymus and an increase in ALT and AST activity levels without any changes in corticosterone level. After the injection of high-dose LPS, the inflammatory and pathological changes in the target organs manifested as severe endotoxemia and sex differences of pathological changes in the lungs and liver were not revealed. The level of production of IL-2, IL-4, IFNγ, and TNF by splenic cells and the number of T-lymphocytes, including cytotoxic cells, in the peripheral blood, decreased in males, which is an evidence of a pronounced suppression of the immune response. Conclusion: We have shown that the morphofunctional changes in the organs of the immune system in females and males, as well as the intensity of the sex differences of inflammation, depend on the severity of systemic inflammatory response, induced by different doses of LPS. Keywords: endotoxinemia, SIRS, thymus, spleen, male rats, female ratsKosyreva AMMakarova OVKakturskiy LVMikhailova LPBoltovskaya MNRogov KADove Medical Pressarticlesex differencesinflammationSIRSendotoxinhigh- and low-dose LPSPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 11, Pp 431-445 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	sex differences inflammation SIRS endotoxin high- and low-dose LPS Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	sex differences inflammation SIRS endotoxin high- and low-dose LPS Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Kosyreva AM Makarova OV Kakturskiy LV Mikhailova LP Boltovskaya MN Rogov KA Sex differences of inflammation in target organs, induced intraperitoneal injection of lipopolysaccharide, depend on its dose
description	Anna M Kosyreva,1 Olga V Makarova,1 Lev V Kakturskiy,2 Liliya P Mikhailova,1 Marina N Boltovskaya,3 Konstantin A Rogov2 1Department of Immunomorphology of Inflammation, Federal State Budgetary Institution “Science Research Institute of Human Morphology”, Moscow, Russia; 2Department of Pathology, Federal State Budgetary Institution “Science Research Institute of Human Morphology”, Moscow, Russia; 3Department of Reproductive Pathology, Federal State Budgetary Institution “Science Research Institute of Human Morphology”, Moscow, Russia Purpose: The aim of our research was to study sex differences and the severity of inflammatory changes in target organs and the peculiarities of immunological disorders when low and high doses of lipopolysaccharide (LPS) were administered to rats. Methods: Male and female 2- to 3-month-old Wistar rats (200–250 g) were injected intraperitoneally with Escherichia coli LPS in one of two doses: 1.5 or 15 mg/kg. In a day after the LPS injection, we studied endotoxin, corticosterone, sex steroids, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) activity levels in the serum; morphological disorders in the lung, liver, thymus, and spleen; ex vivo production of IL-2, IL-4, tumor necrosis factor (TNF), and interferon γ (IFNγ) by splenic cells activated by ConA; and relative amount of T- and B-lymphocytes in the peripheral blood. Results: After the injection of low-dose LPS, the serum endotoxin level increased only in males and was combined with a more pronounced inflammatory response in the lungs and thymus and an increase in ALT and AST activity levels without any changes in corticosterone level. After the injection of high-dose LPS, the inflammatory and pathological changes in the target organs manifested as severe endotoxemia and sex differences of pathological changes in the lungs and liver were not revealed. The level of production of IL-2, IL-4, IFNγ, and TNF by splenic cells and the number of T-lymphocytes, including cytotoxic cells, in the peripheral blood, decreased in males, which is an evidence of a pronounced suppression of the immune response. Conclusion: We have shown that the morphofunctional changes in the organs of the immune system in females and males, as well as the intensity of the sex differences of inflammation, depend on the severity of systemic inflammatory response, induced by different doses of LPS. Keywords: endotoxinemia, SIRS, thymus, spleen, male rats, female rats
format	article
author	Kosyreva AM Makarova OV Kakturskiy LV Mikhailova LP Boltovskaya MN Rogov KA
author_facet	Kosyreva AM Makarova OV Kakturskiy LV Mikhailova LP Boltovskaya MN Rogov KA
author_sort	Kosyreva AM
title	Sex differences of inflammation in target organs, induced intraperitoneal injection of lipopolysaccharide, depend on its dose
title_short	Sex differences of inflammation in target organs, induced intraperitoneal injection of lipopolysaccharide, depend on its dose
title_full	Sex differences of inflammation in target organs, induced intraperitoneal injection of lipopolysaccharide, depend on its dose
title_fullStr	Sex differences of inflammation in target organs, induced intraperitoneal injection of lipopolysaccharide, depend on its dose
title_full_unstemmed	Sex differences of inflammation in target organs, induced intraperitoneal injection of lipopolysaccharide, depend on its dose
title_sort	sex differences of inflammation in target organs, induced intraperitoneal injection of lipopolysaccharide, depend on its dose
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/dfb2921088b74bebb99bab7e9e6fa31c
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_version_	1718402012546072576

Sex differences of inflammation in target organs, induced intraperitoneal injection of lipopolysaccharide, depend on its dose

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