Expression of the domain cassette 8 Plasmodium falciparum erythrocyte membrane protein 1 is associated with cerebral malaria in Benin.

<h4>Background</h4>Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP-1) is a highly polymorphic adherence receptor expressed on the surface of infected erythrocytes. Based on sequence homology PfEMP-1 variants have been grouped into three major groups A-C, the highly conserved...

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Autores principales:	Gwladys I Bertin, Thomas Lavstsen, François Guillonneau, Justin Doritchamou, Christian W Wang, Jakob S Jespersen, Sem Ezimegnon, Nadine Fievet, Maroufou J Alao, Francis Lalya, Achille Massougbodji, Nicaise Tuikue Ndam, Thor G Theander, Philippe Deloron
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dfb7f7390df74ddeb667f3c6ba2899b1
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spelling	oai:doaj.org-article:dfb7f7390df74ddeb667f3c6ba2899b12021-11-18T09:02:31ZExpression of the domain cassette 8 Plasmodium falciparum erythrocyte membrane protein 1 is associated with cerebral malaria in Benin.1932-620310.1371/journal.pone.0068368https://doaj.org/article/dfb7f7390df74ddeb667f3c6ba2899b12013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23922654/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP-1) is a highly polymorphic adherence receptor expressed on the surface of infected erythrocytes. Based on sequence homology PfEMP-1 variants have been grouped into three major groups A-C, the highly conserved VAR2CSA variants, and semi-conserved types defined by tandem runs of specific domains ("domain cassettes" (DC)). The PfEMP-1 type expressed determines the adherence phenotype, and is associated with clinical outcome of infection.<h4>Methods</h4>Parasite isolates from Beninese children or women presenting with, respectively, CM or PAM were collected along with samples from patients with uncomplicated malaria (UM). We assessed the transcript level of var genes by RT-qPCR and the expression of PfEMP-1 proteins by LC-MS/MS.<h4>Results</h4>Var genes encoding DC8 and Group A PfEMP-1 were transcribed more often and at higher levels in cerebral malaria vs. uncomplicated malaria patients. LC-MS/MS identified peptides from group A, DC8 PfEMP-1 more frequently in cerebral malaria than in uncomplicated malaria and pregnancy-associated malaria samples.<h4>Conclusion</h4>This is the first study to show association between PfEMP-1 subtype and disease outcome by direct analysis of parasites proteome. The results corroborate that group A and specifically the PfEMP-1 types DC8 are universally associated with cerebral malaria. This is a crucial observation for promoting studies on malaria pathogenesis.Gwladys I BertinThomas LavstsenFrançois GuillonneauJustin DoritchamouChristian W WangJakob S JespersenSem EzimegnonNadine FievetMaroufou J AlaoFrancis LalyaAchille MassougbodjiNicaise Tuikue NdamThor G TheanderPhilippe DeloronPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e68368 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gwladys I Bertin Thomas Lavstsen François Guillonneau Justin Doritchamou Christian W Wang Jakob S Jespersen Sem Ezimegnon Nadine Fievet Maroufou J Alao Francis Lalya Achille Massougbodji Nicaise Tuikue Ndam Thor G Theander Philippe Deloron Expression of the domain cassette 8 Plasmodium falciparum erythrocyte membrane protein 1 is associated with cerebral malaria in Benin.
description	<h4>Background</h4>Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP-1) is a highly polymorphic adherence receptor expressed on the surface of infected erythrocytes. Based on sequence homology PfEMP-1 variants have been grouped into three major groups A-C, the highly conserved VAR2CSA variants, and semi-conserved types defined by tandem runs of specific domains ("domain cassettes" (DC)). The PfEMP-1 type expressed determines the adherence phenotype, and is associated with clinical outcome of infection.<h4>Methods</h4>Parasite isolates from Beninese children or women presenting with, respectively, CM or PAM were collected along with samples from patients with uncomplicated malaria (UM). We assessed the transcript level of var genes by RT-qPCR and the expression of PfEMP-1 proteins by LC-MS/MS.<h4>Results</h4>Var genes encoding DC8 and Group A PfEMP-1 were transcribed more often and at higher levels in cerebral malaria vs. uncomplicated malaria patients. LC-MS/MS identified peptides from group A, DC8 PfEMP-1 more frequently in cerebral malaria than in uncomplicated malaria and pregnancy-associated malaria samples.<h4>Conclusion</h4>This is the first study to show association between PfEMP-1 subtype and disease outcome by direct analysis of parasites proteome. The results corroborate that group A and specifically the PfEMP-1 types DC8 are universally associated with cerebral malaria. This is a crucial observation for promoting studies on malaria pathogenesis.
format	article
author	Gwladys I Bertin Thomas Lavstsen François Guillonneau Justin Doritchamou Christian W Wang Jakob S Jespersen Sem Ezimegnon Nadine Fievet Maroufou J Alao Francis Lalya Achille Massougbodji Nicaise Tuikue Ndam Thor G Theander Philippe Deloron
author_facet	Gwladys I Bertin Thomas Lavstsen François Guillonneau Justin Doritchamou Christian W Wang Jakob S Jespersen Sem Ezimegnon Nadine Fievet Maroufou J Alao Francis Lalya Achille Massougbodji Nicaise Tuikue Ndam Thor G Theander Philippe Deloron
author_sort	Gwladys I Bertin
title	Expression of the domain cassette 8 Plasmodium falciparum erythrocyte membrane protein 1 is associated with cerebral malaria in Benin.
title_short	Expression of the domain cassette 8 Plasmodium falciparum erythrocyte membrane protein 1 is associated with cerebral malaria in Benin.
title_full	Expression of the domain cassette 8 Plasmodium falciparum erythrocyte membrane protein 1 is associated with cerebral malaria in Benin.
title_fullStr	Expression of the domain cassette 8 Plasmodium falciparum erythrocyte membrane protein 1 is associated with cerebral malaria in Benin.
title_full_unstemmed	Expression of the domain cassette 8 Plasmodium falciparum erythrocyte membrane protein 1 is associated with cerebral malaria in Benin.
title_sort	expression of the domain cassette 8 plasmodium falciparum erythrocyte membrane protein 1 is associated with cerebral malaria in benin.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/dfb7f7390df74ddeb667f3c6ba2899b1
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Expression of the domain cassette 8 Plasmodium falciparum erythrocyte membrane protein 1 is associated with cerebral malaria in Benin.

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