Molecular Proof of a Clinical Concept: Expression of Estrogen Alpha-, Beta-Receptors and G Protein-Coupled Estrogen Receptor 1 (GPER) in Histologically Assessed Common Nevi, Dysplastic Nevi and Melanomas

<i>Background and Objectives:</i> Epidemiologic data show significant differences in melanoma incidence and outcomes between sexes. The role of hormonal receptors in the pathogenesis of melanocytic lesions remains unclear, thus we performed this study aiming to assess estrogen receptors...

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Autores principales:	Magdalena Spałkowska, Grzegorz Dyduch, Elżbieta Broniatowska, Giovanni Damiani, Anna Wojas-Pelc
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	melanoma dysplastic nevus estrogen GPER estrogen receptor gender Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/dfb8572bb1c84e39836fd8d03a72b7ff
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spelling	oai:doaj.org-article:dfb8572bb1c84e39836fd8d03a72b7ff2021-11-25T18:18:46ZMolecular Proof of a Clinical Concept: Expression of Estrogen Alpha-, Beta-Receptors and G Protein-Coupled Estrogen Receptor 1 (GPER) in Histologically Assessed Common Nevi, Dysplastic Nevi and Melanomas10.3390/medicina571112281648-91441010-660Xhttps://doaj.org/article/dfb8572bb1c84e39836fd8d03a72b7ff2021-11-01T00:00:00Zhttps://www.mdpi.com/1648-9144/57/11/1228https://doaj.org/toc/1010-660Xhttps://doaj.org/toc/1648-9144<i>Background and Objectives:</i> Epidemiologic data show significant differences in melanoma incidence and outcomes between sexes. The role of hormonal receptors in the pathogenesis of melanocytic lesions remains unclear, thus we performed this study aiming to assess estrogen receptors expression in different melanocytic lesions. <i>Materials and Methods</i><i>:</i> We performed a cross-sectional study that included 73 consecutively excised melanocytic lesions. Estrogen receptor alpha (ERα), beta (ERβ), and G-protein coupled estrogen receptor (GPER) expression was analyzed in melanocytes and keratinocytes of common nevi, dysplastic nevi, melanoma, healthy skin margin, and in sebaceous and sweat gland cells. <i>Results</i><i>:</i> ERβ expression was higher in dysplastic nevi margin melanocytes compared to common nevi (<i>p</i> = 0.046) and in dysplastic nevi keratinocytes compared to melanoma keratinocytes (<i>p</i> = 0.021). ERβ expression was significantly higher in margin melanocytes compared to melanoma melanocytes (<i>p</i> = 0.009). No difference in ERβ expression was shown between melanocytes of three types of lesions. GPER expression was higher in nuclei and cytoplasm of dysplastic nevi (<i>p</i> = 0.02 and <i>p</i> = 0.036 respectively) and at the margin compared to melanoma. GPER expression was lower in sebaceous glands of tissue surrounding common nevi (<i>p</i> = 0.025) compared to dysplastic nevi. GPER expression was higher in skin margin tissue melanocytes (<i>p</i> = 0.016 nuclear, <i>p</i> = 0.029 cytoplasmic) compared to melanoma melanocytes. There were no differences in ERα expression between the melanocytic lesions. <i>Conclusion:</i> Further large-scale studies are warranted to investigate the potential role of ERβ and GPER in the pathogenesis of melanocytic lesions.Magdalena SpałkowskaGrzegorz DyduchElżbieta BroniatowskaGiovanni DamianiAnna Wojas-PelcMDPI AGarticlemelanomadysplastic nevusestrogenGPERestrogen receptorgenderMedicine (General)R5-920ENMedicina, Vol 57, Iss 1228, p 1228 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	melanoma dysplastic nevus estrogen GPER estrogen receptor gender Medicine (General) R5-920
spellingShingle	melanoma dysplastic nevus estrogen GPER estrogen receptor gender Medicine (General) R5-920 Magdalena Spałkowska Grzegorz Dyduch Elżbieta Broniatowska Giovanni Damiani Anna Wojas-Pelc Molecular Proof of a Clinical Concept: Expression of Estrogen Alpha-, Beta-Receptors and G Protein-Coupled Estrogen Receptor 1 (GPER) in Histologically Assessed Common Nevi, Dysplastic Nevi and Melanomas
description	<i>Background and Objectives:</i> Epidemiologic data show significant differences in melanoma incidence and outcomes between sexes. The role of hormonal receptors in the pathogenesis of melanocytic lesions remains unclear, thus we performed this study aiming to assess estrogen receptors expression in different melanocytic lesions. <i>Materials and Methods</i><i>:</i> We performed a cross-sectional study that included 73 consecutively excised melanocytic lesions. Estrogen receptor alpha (ERα), beta (ERβ), and G-protein coupled estrogen receptor (GPER) expression was analyzed in melanocytes and keratinocytes of common nevi, dysplastic nevi, melanoma, healthy skin margin, and in sebaceous and sweat gland cells. <i>Results</i><i>:</i> ERβ expression was higher in dysplastic nevi margin melanocytes compared to common nevi (<i>p</i> = 0.046) and in dysplastic nevi keratinocytes compared to melanoma keratinocytes (<i>p</i> = 0.021). ERβ expression was significantly higher in margin melanocytes compared to melanoma melanocytes (<i>p</i> = 0.009). No difference in ERβ expression was shown between melanocytes of three types of lesions. GPER expression was higher in nuclei and cytoplasm of dysplastic nevi (<i>p</i> = 0.02 and <i>p</i> = 0.036 respectively) and at the margin compared to melanoma. GPER expression was lower in sebaceous glands of tissue surrounding common nevi (<i>p</i> = 0.025) compared to dysplastic nevi. GPER expression was higher in skin margin tissue melanocytes (<i>p</i> = 0.016 nuclear, <i>p</i> = 0.029 cytoplasmic) compared to melanoma melanocytes. There were no differences in ERα expression between the melanocytic lesions. <i>Conclusion:</i> Further large-scale studies are warranted to investigate the potential role of ERβ and GPER in the pathogenesis of melanocytic lesions.
format	article
author	Magdalena Spałkowska Grzegorz Dyduch Elżbieta Broniatowska Giovanni Damiani Anna Wojas-Pelc
author_facet	Magdalena Spałkowska Grzegorz Dyduch Elżbieta Broniatowska Giovanni Damiani Anna Wojas-Pelc
author_sort	Magdalena Spałkowska
title	Molecular Proof of a Clinical Concept: Expression of Estrogen Alpha-, Beta-Receptors and G Protein-Coupled Estrogen Receptor 1 (GPER) in Histologically Assessed Common Nevi, Dysplastic Nevi and Melanomas
title_short	Molecular Proof of a Clinical Concept: Expression of Estrogen Alpha-, Beta-Receptors and G Protein-Coupled Estrogen Receptor 1 (GPER) in Histologically Assessed Common Nevi, Dysplastic Nevi and Melanomas
title_full	Molecular Proof of a Clinical Concept: Expression of Estrogen Alpha-, Beta-Receptors and G Protein-Coupled Estrogen Receptor 1 (GPER) in Histologically Assessed Common Nevi, Dysplastic Nevi and Melanomas
title_fullStr	Molecular Proof of a Clinical Concept: Expression of Estrogen Alpha-, Beta-Receptors and G Protein-Coupled Estrogen Receptor 1 (GPER) in Histologically Assessed Common Nevi, Dysplastic Nevi and Melanomas
title_full_unstemmed	Molecular Proof of a Clinical Concept: Expression of Estrogen Alpha-, Beta-Receptors and G Protein-Coupled Estrogen Receptor 1 (GPER) in Histologically Assessed Common Nevi, Dysplastic Nevi and Melanomas
title_sort	molecular proof of a clinical concept: expression of estrogen alpha-, beta-receptors and g protein-coupled estrogen receptor 1 (gper) in histologically assessed common nevi, dysplastic nevi and melanomas
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/dfb8572bb1c84e39836fd8d03a72b7ff
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Molecular Proof of a Clinical Concept: Expression of Estrogen Alpha-, Beta-Receptors and G Protein-Coupled Estrogen Receptor 1 (GPER) in Histologically Assessed Common Nevi, Dysplastic Nevi and Melanomas

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