A biomimetic peptide has no effect on the isotopic fractionation during in vitro silica precipitation

A biomimetic peptide has no effect on the isotopic fractionation during in vitro silica precipitation

Abstract The stable isotopic composition of diatom silica is used as a proxy for nutrient utilisation in natural waters. This approach provides essential insight into the current and historic links between biological production, carbon cycling and climate. However, estimates of isotopic fractionatio...

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Autores principales: Lucie Cassarino, Paul Curnow, Katharine R. Hendry
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/dfbad14712b14eb79d46f8c2bd097a2f
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spelling oai:doaj.org-article:dfbad14712b14eb79d46f8c2bd097a2f2021-12-02T15:38:11ZA biomimetic peptide has no effect on the isotopic fractionation during in vitro silica precipitation10.1038/s41598-021-88881-62045-2322https://doaj.org/article/dfbad14712b14eb79d46f8c2bd097a2f2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88881-6https://doaj.org/toc/2045-2322Abstract The stable isotopic composition of diatom silica is used as a proxy for nutrient utilisation in natural waters. This approach provides essential insight into the current and historic links between biological production, carbon cycling and climate. However, estimates of isotopic fractionation during diatom silica production from both laboratory and field studies are variable, and the biochemical pathways responsible remain unknown. Here, we investigate silicon isotopic fractionation through a series of chemical precipitation experiments that are analogous to the first stages of intracellular silica formation within the diatom silicon deposition vesicle. The novelty of our experiment is the inclusion of the R5 peptide, which is closely related to a natural biomolecule known to play a role in diatom silicification. Our results suggest that the presence of R5 induces a systematic but non-significant difference in fractionation behaviour. It thus appears that silicon isotopic fractionation in vitro is largely driven by an early kinetic fractionation during rapid precipitation that correlates with the initial amount of dissolved silica in the system. Our findings raise the question of how environmental changes might impact silicon isotopic fractionation in diatoms, and whether frustule archives record information in addition to silica consumption in surface water.Lucie CassarinoPaul CurnowKatharine R. HendryNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Lucie Cassarino
Paul Curnow
Katharine R. Hendry
A biomimetic peptide has no effect on the isotopic fractionation during in vitro silica precipitation
description Abstract The stable isotopic composition of diatom silica is used as a proxy for nutrient utilisation in natural waters. This approach provides essential insight into the current and historic links between biological production, carbon cycling and climate. However, estimates of isotopic fractionation during diatom silica production from both laboratory and field studies are variable, and the biochemical pathways responsible remain unknown. Here, we investigate silicon isotopic fractionation through a series of chemical precipitation experiments that are analogous to the first stages of intracellular silica formation within the diatom silicon deposition vesicle. The novelty of our experiment is the inclusion of the R5 peptide, which is closely related to a natural biomolecule known to play a role in diatom silicification. Our results suggest that the presence of R5 induces a systematic but non-significant difference in fractionation behaviour. It thus appears that silicon isotopic fractionation in vitro is largely driven by an early kinetic fractionation during rapid precipitation that correlates with the initial amount of dissolved silica in the system. Our findings raise the question of how environmental changes might impact silicon isotopic fractionation in diatoms, and whether frustule archives record information in addition to silica consumption in surface water.
format article
author Lucie Cassarino
Paul Curnow
Katharine R. Hendry
author_facet Lucie Cassarino
Paul Curnow
Katharine R. Hendry
author_sort Lucie Cassarino
title A biomimetic peptide has no effect on the isotopic fractionation during in vitro silica precipitation
title_short A biomimetic peptide has no effect on the isotopic fractionation during in vitro silica precipitation
title_full A biomimetic peptide has no effect on the isotopic fractionation during in vitro silica precipitation
title_fullStr A biomimetic peptide has no effect on the isotopic fractionation during in vitro silica precipitation
title_full_unstemmed A biomimetic peptide has no effect on the isotopic fractionation during in vitro silica precipitation
title_sort biomimetic peptide has no effect on the isotopic fractionation during in vitro silica precipitation
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/dfbad14712b14eb79d46f8c2bd097a2f
work_keys_str_mv AT luciecassarino abiomimeticpeptidehasnoeffectontheisotopicfractionationduringinvitrosilicaprecipitation
AT paulcurnow abiomimeticpeptidehasnoeffectontheisotopicfractionationduringinvitrosilicaprecipitation
AT katharinerhendry abiomimeticpeptidehasnoeffectontheisotopicfractionationduringinvitrosilicaprecipitation
AT luciecassarino biomimeticpeptidehasnoeffectontheisotopicfractionationduringinvitrosilicaprecipitation
AT paulcurnow biomimeticpeptidehasnoeffectontheisotopicfractionationduringinvitrosilicaprecipitation
AT katharinerhendry biomimeticpeptidehasnoeffectontheisotopicfractionationduringinvitrosilicaprecipitation
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